Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo)
Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected B. bufo e...
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ftulundlup:oai:lup.lub.lu.se:b1e8cfd3-ace5-42cc-a6ef-68a69b0bd6a8 2024-05-19T07:46:08+00:00 Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) Chondrelli, Niki Kuehn, Emily Meurling, Sara Cortázar-Chinarro, Maria Laurila, Anssi Höglund, Jacob 2024-01 https://lup.lub.lu.se/record/b1e8cfd3-ace5-42cc-a6ef-68a69b0bd6a8 https://doi.org/10.1038/s41598-024-52975-8 eng eng Nature Publishing Group https://lup.lub.lu.se/record/b1e8cfd3-ace5-42cc-a6ef-68a69b0bd6a8 http://dx.doi.org/10.1038/s41598-024-52975-8 scopus:85183627769 pmid:38291226 Scientific Reports; 14, no 2495 (2024) ISSN: 2045-2322 Microbiology Genetics contributiontojournal/article info:eu-repo/semantics/article text 2024 ftulundlup https://doi.org/10.1038/s41598-024-52975-8 2024-04-24T00:00:22Z Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected B. bufo eggs in southern and northern Sweden, and infected the laboratory-raised metamorphs with two strains of the global panzoonotic lineage Bd-GPL. Differential expression analysis showed significant differences between infected and control individuals in both liver and skin. The skin samples showed no discernible differences in gene expression between the two strains used, while liver samples were differentiated by strain, with one of the strains eliciting no immune response from infected toads. Immune system genes were overexpressed in skin samples from surviving infected individuals, while in liver samples the pattern was more diffuse. Splitting samples by population revealed a stronger immune response in northern individuals. Differences in transcriptional regulation between populations are particularly relevant to study in Swedish amphibians, which may have experienced varying exposure to Bd. Earlier exposure to this pathogen and subsequent adaptation or selection pressure may contribute to the survival of some populations over others, while standing genetic diversity in different populations may also affect the infection outcome. Article in Journal/Newspaper Northern Sweden Lund University Publications (LUP) Scientific Reports 14 1 |
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Lund University Publications (LUP) |
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ftulundlup |
language |
English |
topic |
Microbiology Genetics |
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Microbiology Genetics Chondrelli, Niki Kuehn, Emily Meurling, Sara Cortázar-Chinarro, Maria Laurila, Anssi Höglund, Jacob Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
topic_facet |
Microbiology Genetics |
description |
Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected B. bufo eggs in southern and northern Sweden, and infected the laboratory-raised metamorphs with two strains of the global panzoonotic lineage Bd-GPL. Differential expression analysis showed significant differences between infected and control individuals in both liver and skin. The skin samples showed no discernible differences in gene expression between the two strains used, while liver samples were differentiated by strain, with one of the strains eliciting no immune response from infected toads. Immune system genes were overexpressed in skin samples from surviving infected individuals, while in liver samples the pattern was more diffuse. Splitting samples by population revealed a stronger immune response in northern individuals. Differences in transcriptional regulation between populations are particularly relevant to study in Swedish amphibians, which may have experienced varying exposure to Bd. Earlier exposure to this pathogen and subsequent adaptation or selection pressure may contribute to the survival of some populations over others, while standing genetic diversity in different populations may also affect the infection outcome. |
format |
Article in Journal/Newspaper |
author |
Chondrelli, Niki Kuehn, Emily Meurling, Sara Cortázar-Chinarro, Maria Laurila, Anssi Höglund, Jacob |
author_facet |
Chondrelli, Niki Kuehn, Emily Meurling, Sara Cortázar-Chinarro, Maria Laurila, Anssi Höglund, Jacob |
author_sort |
Chondrelli, Niki |
title |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_short |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_full |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_fullStr |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_full_unstemmed |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_sort |
batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (bufo bufo) |
publisher |
Nature Publishing Group |
publishDate |
2024 |
url |
https://lup.lub.lu.se/record/b1e8cfd3-ace5-42cc-a6ef-68a69b0bd6a8 https://doi.org/10.1038/s41598-024-52975-8 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Scientific Reports; 14, no 2495 (2024) ISSN: 2045-2322 |
op_relation |
https://lup.lub.lu.se/record/b1e8cfd3-ace5-42cc-a6ef-68a69b0bd6a8 http://dx.doi.org/10.1038/s41598-024-52975-8 scopus:85183627769 pmid:38291226 |
op_doi |
https://doi.org/10.1038/s41598-024-52975-8 |
container_title |
Scientific Reports |
container_volume |
14 |
container_issue |
1 |
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1799486262408642560 |