Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations.
Persistent organic pollutants (POPs), such as PCBs (polychlorinated biphenyls) and DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane], are environmental contaminants with potential endocrine disrupting activity. DNA methylation levels in peripheral blood lymphocytes have been associated with serum...
Published in: | Environmental and Molecular Mutagenesis |
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Main Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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John Wiley & Sons Inc.
2016
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Online Access: | https://lup.lub.lu.se/record/8574031 https://doi.org/10.1002/em.21994 |
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ftulundlup:oai:lup.lub.lu.se:b0ff24fd-a63f-468c-8a25-cf970983e3f5 2023-05-15T15:12:11+02:00 Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. Consales, Claudia Toft, Gunnar Leter, Giorgio Bonde, Jens Peter E Uccelli, Raffaella Pacchierotti, Francesca Eleuteri, Patrizia Jönsson, Bo A Giwercman, Aleksander Pedersen, Henning S Stuciński, Paweł Góralczyk, Katarzyna Zviezdai, Valentyna Spanò, Marcello 2016-01-23 https://lup.lub.lu.se/record/8574031 https://doi.org/10.1002/em.21994 eng eng John Wiley & Sons Inc. https://lup.lub.lu.se/record/8574031 http://dx.doi.org/10.1002/em.21994 pmid:26801515 scopus:84960325114 wos:000372722100003 Environmental and Molecular Mutagenesis; 57(3), pp 200-209 (2016) ISSN: 1098-2280 Environmental Health and Occupational Health contributiontojournal/article info:eu-repo/semantics/article text 2016 ftulundlup https://doi.org/10.1002/em.21994 2023-02-01T23:30:06Z Persistent organic pollutants (POPs), such as PCBs (polychlorinated biphenyls) and DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane], are environmental contaminants with potential endocrine disrupting activity. DNA methylation levels in peripheral blood lymphocytes have been associated with serum concentrations of POPs in Greenland Inuit and Korean populations. Greenland Inuits are characterized by the highest worldwide POP levels. In this cross-sectional study we evaluated the relationship between serum POP concentrations and DNA methylation levels in sperm of non-occupationally exposed fertile men from Greenland, Warsaw (Poland), and Kharkiv (Ukraine). Serum levels of PCB-153 [1,2,4-trichloro-5-(2,4,5-trichlorophenyl)benzene], as a proxy of the total PCBs body burden, and of p,p'-DDE [1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene], the main metabolite of DDT were measured. Sperm DNA methylation level was assessed globally by flow cytometric (FCM) immunodetection of 5-methyl-cytosines and at specific repetitive DNA sequences (Alu, LINE-1, Satα) by PCR-pyrosequencing after bisulfite conversion. Multivariate linear regression analysis was applied to investigate correlations between serum POP concentrations and DNA methylation. No consistent associations between exposure to POPs and sperm DNA methylation at repetitive DNA sequences were detected. A statistically significant global decrease in methylation was associated with exposure to either POP by FCM analysis. This is the first study to investigate environmental exposure to POPs and DNA methylation levels considering sperm as the target cells. Although POP exposure appears to have a limited negative impact on sperm DNA methylation levels in adult males, the global hypomethylation detected by one of the methods applied suggests that further investigation is warranted. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc. Article in Journal/Newspaper Arctic Greenland inuit inuits Lund University Publications (LUP) Arctic Greenland Environmental and Molecular Mutagenesis 57 3 200 209 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Environmental Health and Occupational Health |
spellingShingle |
Environmental Health and Occupational Health Consales, Claudia Toft, Gunnar Leter, Giorgio Bonde, Jens Peter E Uccelli, Raffaella Pacchierotti, Francesca Eleuteri, Patrizia Jönsson, Bo A Giwercman, Aleksander Pedersen, Henning S Stuciński, Paweł Góralczyk, Katarzyna Zviezdai, Valentyna Spanò, Marcello Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. |
topic_facet |
Environmental Health and Occupational Health |
description |
Persistent organic pollutants (POPs), such as PCBs (polychlorinated biphenyls) and DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane], are environmental contaminants with potential endocrine disrupting activity. DNA methylation levels in peripheral blood lymphocytes have been associated with serum concentrations of POPs in Greenland Inuit and Korean populations. Greenland Inuits are characterized by the highest worldwide POP levels. In this cross-sectional study we evaluated the relationship between serum POP concentrations and DNA methylation levels in sperm of non-occupationally exposed fertile men from Greenland, Warsaw (Poland), and Kharkiv (Ukraine). Serum levels of PCB-153 [1,2,4-trichloro-5-(2,4,5-trichlorophenyl)benzene], as a proxy of the total PCBs body burden, and of p,p'-DDE [1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene], the main metabolite of DDT were measured. Sperm DNA methylation level was assessed globally by flow cytometric (FCM) immunodetection of 5-methyl-cytosines and at specific repetitive DNA sequences (Alu, LINE-1, Satα) by PCR-pyrosequencing after bisulfite conversion. Multivariate linear regression analysis was applied to investigate correlations between serum POP concentrations and DNA methylation. No consistent associations between exposure to POPs and sperm DNA methylation at repetitive DNA sequences were detected. A statistically significant global decrease in methylation was associated with exposure to either POP by FCM analysis. This is the first study to investigate environmental exposure to POPs and DNA methylation levels considering sperm as the target cells. Although POP exposure appears to have a limited negative impact on sperm DNA methylation levels in adult males, the global hypomethylation detected by one of the methods applied suggests that further investigation is warranted. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Consales, Claudia Toft, Gunnar Leter, Giorgio Bonde, Jens Peter E Uccelli, Raffaella Pacchierotti, Francesca Eleuteri, Patrizia Jönsson, Bo A Giwercman, Aleksander Pedersen, Henning S Stuciński, Paweł Góralczyk, Katarzyna Zviezdai, Valentyna Spanò, Marcello |
author_facet |
Consales, Claudia Toft, Gunnar Leter, Giorgio Bonde, Jens Peter E Uccelli, Raffaella Pacchierotti, Francesca Eleuteri, Patrizia Jönsson, Bo A Giwercman, Aleksander Pedersen, Henning S Stuciński, Paweł Góralczyk, Katarzyna Zviezdai, Valentyna Spanò, Marcello |
author_sort |
Consales, Claudia |
title |
Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. |
title_short |
Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. |
title_full |
Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. |
title_fullStr |
Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. |
title_full_unstemmed |
Exposure to persistent organic pollutants and sperm DNA methylation changes in Arctic and European populations. |
title_sort |
exposure to persistent organic pollutants and sperm dna methylation changes in arctic and european populations. |
publisher |
John Wiley & Sons Inc. |
publishDate |
2016 |
url |
https://lup.lub.lu.se/record/8574031 https://doi.org/10.1002/em.21994 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland inuit inuits |
genre_facet |
Arctic Greenland inuit inuits |
op_source |
Environmental and Molecular Mutagenesis; 57(3), pp 200-209 (2016) ISSN: 1098-2280 |
op_relation |
https://lup.lub.lu.se/record/8574031 http://dx.doi.org/10.1002/em.21994 pmid:26801515 scopus:84960325114 wos:000372722100003 |
op_doi |
https://doi.org/10.1002/em.21994 |
container_title |
Environmental and Molecular Mutagenesis |
container_volume |
57 |
container_issue |
3 |
container_start_page |
200 |
op_container_end_page |
209 |
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1766342899221397504 |