Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil
This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Faculty of Food Technology and Biotechnology University
2008
|
Subjects: | |
Online Access: | https://lup.lub.lu.se/record/1186938 |
id |
ftulundlup:oai:lup.lub.lu.se:a45d16de-25c0-44b2-a998-c3b9257f2da2 |
---|---|
record_format |
openpolar |
spelling |
ftulundlup:oai:lup.lub.lu.se:a45d16de-25c0-44b2-a998-c3b9257f2da2 2023-05-15T13:57:46+02:00 Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil Venskutonis, Petras Rimantas Dauksas, Egidijus Sivik, Björn 2008 https://lup.lub.lu.se/record/1186938 eng eng Faculty of Food Technology and Biotechnology University https://lup.lub.lu.se/record/1186938 wos:000257058900005 scopus:46349099662 Food Technology and Biotechnology; 46(2), pp 157-163 (2008) ISSN: 1330-9862 Engineering and Technology entrainer supercritical CO2 extraction borage Candida antarctica contributiontojournal/article info:eu-repo/semantics/article text 2008 ftulundlup 2023-02-01T23:33:10Z This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first extractor was loaded with ground borage seeds, the second one was filled with the enzyme. The total yield obtained at 15, 25 and 35 MPa was (8.8+/-0.2), (23.6+/-0.2) and (28.9+/-1.1) %, respectively. Thin layer chromatography (TLC) of fatty acid ethyl esters showed that the content of esters was higher in the extract obtained in one extractor system at 15 MPa, compared to 35 MPa. Article in Journal/Newspaper Antarc* Antarctica Lund University Publications (LUP) |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Engineering and Technology entrainer supercritical CO2 extraction borage Candida antarctica |
spellingShingle |
Engineering and Technology entrainer supercritical CO2 extraction borage Candida antarctica Venskutonis, Petras Rimantas Dauksas, Egidijus Sivik, Björn Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
topic_facet |
Engineering and Technology entrainer supercritical CO2 extraction borage Candida antarctica |
description |
This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first extractor was loaded with ground borage seeds, the second one was filled with the enzyme. The total yield obtained at 15, 25 and 35 MPa was (8.8+/-0.2), (23.6+/-0.2) and (28.9+/-1.1) %, respectively. Thin layer chromatography (TLC) of fatty acid ethyl esters showed that the content of esters was higher in the extract obtained in one extractor system at 15 MPa, compared to 35 MPa. |
format |
Article in Journal/Newspaper |
author |
Venskutonis, Petras Rimantas Dauksas, Egidijus Sivik, Björn |
author_facet |
Venskutonis, Petras Rimantas Dauksas, Egidijus Sivik, Björn |
author_sort |
Venskutonis, Petras Rimantas |
title |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_short |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_full |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_fullStr |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_full_unstemmed |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_sort |
use of immobilised lipase from candida antarctica in supercritical fluid extraction of borage (borago officinalis l.) seed oil |
publisher |
Faculty of Food Technology and Biotechnology University |
publishDate |
2008 |
url |
https://lup.lub.lu.se/record/1186938 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Food Technology and Biotechnology; 46(2), pp 157-163 (2008) ISSN: 1330-9862 |
op_relation |
https://lup.lub.lu.se/record/1186938 wos:000257058900005 scopus:46349099662 |
_version_ |
1766265661182443520 |