Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland
Subglacial lakes beneath the Vatnajokull ice cap in Iceland host endemic communities of microorganisms adapted to cold, dark and nutrient-poor waters, but the mechanisms by which these microbes disseminate under the ice and colonize these lakes are unknown. We present new data on this subglacial mic...
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ftulundlup:oai:lup.lub.lu.se:93d84309-cebd-45fc-814f-333f45dcfe21 2023-05-15T16:38:11+02:00 Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland Marteinsson, Viggo Thor Runarsson, Arni Stefansson, Andri Thorsteinsson, Thorsteinn Johannesson, Tomas Magnusson, Sveinn H. Reynisson, Eyjolfur Einarsson, Bergur Wade, Nicole Morrison, Hilary G. Gaidos, Eric 2013 https://lup.lub.lu.se/record/3761091 https://doi.org/10.1038/ismej.2012.97 eng eng Nature Publishing Group https://lup.lub.lu.se/record/3761091 http://dx.doi.org/10.1038/ismej.2012.97 wos:000316723300019 scopus:84872935229 pmid:22975882 The Isme Journal; 7(2), pp 427-437 (2013) ISSN: 1751-7362 Social Sciences Interdisciplinary acetogenesis astrobiology extreme environments psychrophiles pyrosequencing subglacial lakes contributiontojournal/article info:eu-repo/semantics/article text 2013 ftulundlup https://doi.org/10.1038/ismej.2012.97 2023-02-01T23:29:53Z Subglacial lakes beneath the Vatnajokull ice cap in Iceland host endemic communities of microorganisms adapted to cold, dark and nutrient-poor waters, but the mechanisms by which these microbes disseminate under the ice and colonize these lakes are unknown. We present new data on this subglacial microbiome generated from samples of two subglacial lakes, a subglacial flood and a lake that was formerly subglacial but now partly exposed to the atmosphere. These data include parallel 16S rRNA gene amplicon libraries constructed using novel primers that span the v3-v5 and v4-v6 hypervariable regions. Archaea were not detected in either subglacial lake, and the communities are dominated by only five bacterial taxa. Our paired libraries are highly concordant for the most abundant taxa, but estimates of diversity (abundance-based coverage estimator) in the v4-v6 libraries are 3-8 times higher than in corresponding v3-v5 libraries. The dominant taxa are closely related to cultivated anaerobes and microaerobes, and may occupy unique metabolic niches in a chemoautolithotrophic ecosystem. The populations of the major taxa in the subglacial lakes are indistinguishable (>99% sequence identity), despite separation by 6 km and an ice divide; one taxon is ubiquitous in our Vatnajokull samples. We propose that the glacial bed is connected through an aquifer in the underlying permeable basalt, and these subglacial lakes are colonized from a deeper, subterranean microbiome. The ISME Journal (2013) 7, 427-437; doi:10.1038/ismej.2012.97; published online 13 September 2012 Article in Journal/Newspaper Ice cap Iceland Lund University Publications (LUP) The ISME Journal 7 2 427 437 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Social Sciences Interdisciplinary acetogenesis astrobiology extreme environments psychrophiles pyrosequencing subglacial lakes |
spellingShingle |
Social Sciences Interdisciplinary acetogenesis astrobiology extreme environments psychrophiles pyrosequencing subglacial lakes Marteinsson, Viggo Thor Runarsson, Arni Stefansson, Andri Thorsteinsson, Thorsteinn Johannesson, Tomas Magnusson, Sveinn H. Reynisson, Eyjolfur Einarsson, Bergur Wade, Nicole Morrison, Hilary G. Gaidos, Eric Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland |
topic_facet |
Social Sciences Interdisciplinary acetogenesis astrobiology extreme environments psychrophiles pyrosequencing subglacial lakes |
description |
Subglacial lakes beneath the Vatnajokull ice cap in Iceland host endemic communities of microorganisms adapted to cold, dark and nutrient-poor waters, but the mechanisms by which these microbes disseminate under the ice and colonize these lakes are unknown. We present new data on this subglacial microbiome generated from samples of two subglacial lakes, a subglacial flood and a lake that was formerly subglacial but now partly exposed to the atmosphere. These data include parallel 16S rRNA gene amplicon libraries constructed using novel primers that span the v3-v5 and v4-v6 hypervariable regions. Archaea were not detected in either subglacial lake, and the communities are dominated by only five bacterial taxa. Our paired libraries are highly concordant for the most abundant taxa, but estimates of diversity (abundance-based coverage estimator) in the v4-v6 libraries are 3-8 times higher than in corresponding v3-v5 libraries. The dominant taxa are closely related to cultivated anaerobes and microaerobes, and may occupy unique metabolic niches in a chemoautolithotrophic ecosystem. The populations of the major taxa in the subglacial lakes are indistinguishable (>99% sequence identity), despite separation by 6 km and an ice divide; one taxon is ubiquitous in our Vatnajokull samples. We propose that the glacial bed is connected through an aquifer in the underlying permeable basalt, and these subglacial lakes are colonized from a deeper, subterranean microbiome. The ISME Journal (2013) 7, 427-437; doi:10.1038/ismej.2012.97; published online 13 September 2012 |
format |
Article in Journal/Newspaper |
author |
Marteinsson, Viggo Thor Runarsson, Arni Stefansson, Andri Thorsteinsson, Thorsteinn Johannesson, Tomas Magnusson, Sveinn H. Reynisson, Eyjolfur Einarsson, Bergur Wade, Nicole Morrison, Hilary G. Gaidos, Eric |
author_facet |
Marteinsson, Viggo Thor Runarsson, Arni Stefansson, Andri Thorsteinsson, Thorsteinn Johannesson, Tomas Magnusson, Sveinn H. Reynisson, Eyjolfur Einarsson, Bergur Wade, Nicole Morrison, Hilary G. Gaidos, Eric |
author_sort |
Marteinsson, Viggo Thor |
title |
Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland |
title_short |
Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland |
title_full |
Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland |
title_fullStr |
Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland |
title_full_unstemmed |
Microbial communities in the subglacial waters of the Vatnajokull ice cap, Iceland |
title_sort |
microbial communities in the subglacial waters of the vatnajokull ice cap, iceland |
publisher |
Nature Publishing Group |
publishDate |
2013 |
url |
https://lup.lub.lu.se/record/3761091 https://doi.org/10.1038/ismej.2012.97 |
genre |
Ice cap Iceland |
genre_facet |
Ice cap Iceland |
op_source |
The Isme Journal; 7(2), pp 427-437 (2013) ISSN: 1751-7362 |
op_relation |
https://lup.lub.lu.se/record/3761091 http://dx.doi.org/10.1038/ismej.2012.97 wos:000316723300019 scopus:84872935229 pmid:22975882 |
op_doi |
https://doi.org/10.1038/ismej.2012.97 |
container_title |
The ISME Journal |
container_volume |
7 |
container_issue |
2 |
container_start_page |
427 |
op_container_end_page |
437 |
_version_ |
1766028469656879104 |