Towards a cost-effective immobilized lipase for specialty chemicals
Biocatalysis has the potential to provide the chemical industry with several advantages for the production of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately limited due to the high costs related to the production of the biocatalyst. We...
Published in: | Journal of Molecular Catalysis B: Enzymatic |
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Online Access: | https://lup.lub.lu.se/record/1877971 https://doi.org/10.1016/j.molcatb.2010.11.004 |
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ftulundlup:oai:lup.lub.lu.se:8cd03b23-8b73-473f-b4ba-058e1bb5cc67 2023-05-15T13:35:34+02:00 Towards a cost-effective immobilized lipase for specialty chemicals Tufvesson, Pär Törnvall, Ulrika Carvalho, Jorge Karlsson, Annika J. Hatti-Kaul, Rajni 2011 https://lup.lub.lu.se/record/1877971 https://doi.org/10.1016/j.molcatb.2010.11.004 eng eng Elsevier https://lup.lub.lu.se/record/1877971 http://dx.doi.org/10.1016/j.molcatb.2010.11.004 wos:000286778400012 scopus:78650517734 Journal of Molecular Catalysis B: Enzymatic; 68(2), pp 200-205 (2011) ISSN: 1873-3158 Industrial Biotechnology Amidation Esterification Batch stirred tank reactor Packed-bed reactor Biocatalyst stability contributiontojournal/article info:eu-repo/semantics/article text 2011 ftulundlup https://doi.org/10.1016/j.molcatb.2010.11.004 2023-02-01T23:29:25Z Biocatalysis has the potential to provide the chemical industry with several advantages for the production of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately limited due to the high costs related to the production of the biocatalyst. We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly less than the cost of commercially available Novozym (R) 435, indicating a potential for decreased cost. Also the stability of Accurel MP1000 bound enzyme during repeated use matched that of Novozym (R) 435. Finally the in-house biocatalyst was used in a packed-bed set-up, showing an excellent stability in repeated batches at 70 degrees C. 2010 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Antarc* Antarctica Lund University Publications (LUP) Journal of Molecular Catalysis B: Enzymatic 68 2 200 205 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Industrial Biotechnology Amidation Esterification Batch stirred tank reactor Packed-bed reactor Biocatalyst stability |
spellingShingle |
Industrial Biotechnology Amidation Esterification Batch stirred tank reactor Packed-bed reactor Biocatalyst stability Tufvesson, Pär Törnvall, Ulrika Carvalho, Jorge Karlsson, Annika J. Hatti-Kaul, Rajni Towards a cost-effective immobilized lipase for specialty chemicals |
topic_facet |
Industrial Biotechnology Amidation Esterification Batch stirred tank reactor Packed-bed reactor Biocatalyst stability |
description |
Biocatalysis has the potential to provide the chemical industry with several advantages for the production of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately limited due to the high costs related to the production of the biocatalyst. We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly less than the cost of commercially available Novozym (R) 435, indicating a potential for decreased cost. Also the stability of Accurel MP1000 bound enzyme during repeated use matched that of Novozym (R) 435. Finally the in-house biocatalyst was used in a packed-bed set-up, showing an excellent stability in repeated batches at 70 degrees C. 2010 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Tufvesson, Pär Törnvall, Ulrika Carvalho, Jorge Karlsson, Annika J. Hatti-Kaul, Rajni |
author_facet |
Tufvesson, Pär Törnvall, Ulrika Carvalho, Jorge Karlsson, Annika J. Hatti-Kaul, Rajni |
author_sort |
Tufvesson, Pär |
title |
Towards a cost-effective immobilized lipase for specialty chemicals |
title_short |
Towards a cost-effective immobilized lipase for specialty chemicals |
title_full |
Towards a cost-effective immobilized lipase for specialty chemicals |
title_fullStr |
Towards a cost-effective immobilized lipase for specialty chemicals |
title_full_unstemmed |
Towards a cost-effective immobilized lipase for specialty chemicals |
title_sort |
towards a cost-effective immobilized lipase for specialty chemicals |
publisher |
Elsevier |
publishDate |
2011 |
url |
https://lup.lub.lu.se/record/1877971 https://doi.org/10.1016/j.molcatb.2010.11.004 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Molecular Catalysis B: Enzymatic; 68(2), pp 200-205 (2011) ISSN: 1873-3158 |
op_relation |
https://lup.lub.lu.se/record/1877971 http://dx.doi.org/10.1016/j.molcatb.2010.11.004 wos:000286778400012 scopus:78650517734 |
op_doi |
https://doi.org/10.1016/j.molcatb.2010.11.004 |
container_title |
Journal of Molecular Catalysis B: Enzymatic |
container_volume |
68 |
container_issue |
2 |
container_start_page |
200 |
op_container_end_page |
205 |
_version_ |
1766067263465586688 |