Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry
The distribution of neurons expressing preproenkephalin (PPE) mRNA in the brain of the salmon was investigated by means of non-radioactive in situ hybridization, and directly compared with the distribution of enkephalin-immunoreactive (ENKir) neurons. This approach, utilized here for the first time...
Published in: | Journal of Chemical Neuroanatomy |
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Elsevier
1995
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Online Access: | https://lup.lub.lu.se/record/7e6676dd-1786-4a04-aadf-9979983027aa https://doi.org/10.1016/0891-0618(95)00068-I |
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ftulundlup:oai:lup.lub.lu.se:7e6676dd-1786-4a04-aadf-9979983027aa 2024-04-28T08:37:15+00:00 Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry Vecino, E Perez, M T Ekström, P 1995-08 https://lup.lub.lu.se/record/7e6676dd-1786-4a04-aadf-9979983027aa https://doi.org/10.1016/0891-0618(95)00068-I eng eng Elsevier https://lup.lub.lu.se/record/7e6676dd-1786-4a04-aadf-9979983027aa http://dx.doi.org/10.1016/0891-0618(95)00068-I pmid:8561952 scopus:0029151344 Journal of Chemical Neuroanatomy; 9(2), pp 81-97 (1995) ISSN: 0891-0618 Animals Central Nervous System Cobalt Enkephalins Horseradish Peroxidase Immunohistochemistry In Situ Hybridization Lysine Neurons Protein Precursors RNA Messenger Salmon Sensitivity and Specificity Journal Article Research Support Non-U.S. Gov't contributiontojournal/article info:eu-repo/semantics/article text 1995 ftulundlup https://doi.org/10.1016/0891-0618(95)00068-I 2024-04-09T23:34:47Z The distribution of neurons expressing preproenkephalin (PPE) mRNA in the brain of the salmon was investigated by means of non-radioactive in situ hybridization, and directly compared with the distribution of enkephalin-immunoreactive (ENKir) neurons. This approach, utilized here for the first time in a non-mammalian vertebrate for the identification of neurons containing opioid peptides, permitted a detailed analysis of the distribution of putative enkephalinergic neurons in the salmon brain. Several cell groups containing neurons that express PPE mRNA also contain ENKir neurons. Such cell groups are located in the ventral telencephalic area, the nucleus of the rostral mesencephalic tegmentum and another nucleus immediately dorsal to it, the torus semicircularis, the valvula cerebelli and the corpus cerebelli. These cell groups consistently contain larger numbers of PPE mRNA expressing cells than ENKir ones. Some cell groups express PPE mRNA, but do not contain ENKir neurons. These cell groups are located in the dorsal telencephalic area, the inferior lobes of the hypothalamus, the pretectal area, the magnocellular superficial pretectal nucleus, the optic tectum, the oculomotor nucleus, the trochlear nucleus, the magnocellular vestibular nucleus, the secondary gustatory nucleus, the superior and medial reticular nuclei, the motor nucleus of the vagus and the ventral horn of the spinal cord. Moreover, some cell groups contain ENKir neurons, but no PPE mRNA expressing neurons. These cell groups are located in the ventromedial thalamic nucleus, the lateral tuberal nucleus, the nucleus of the lateral recess and the nucleus of the posterior recess. The majority of these periventricular ENKir neurons were of the cerebrospinal fluid-contacting type. ENKir neurons were also located in the dorsal lateral tegmental nucleus and in area B9. The results also permitted a tentative identification of enkephalinergic neurons afferent to the optic tectum, that have previously not been identified with immunocytochemistry, located ... Article in Journal/Newspaper Salmo salar Lund University Publications (LUP) Journal of Chemical Neuroanatomy 9 2 81 97 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Animals Central Nervous System Cobalt Enkephalins Horseradish Peroxidase Immunohistochemistry In Situ Hybridization Lysine Neurons Protein Precursors RNA Messenger Salmon Sensitivity and Specificity Journal Article Research Support Non-U.S. Gov't |
spellingShingle |
Animals Central Nervous System Cobalt Enkephalins Horseradish Peroxidase Immunohistochemistry In Situ Hybridization Lysine Neurons Protein Precursors RNA Messenger Salmon Sensitivity and Specificity Journal Article Research Support Non-U.S. Gov't Vecino, E Perez, M T Ekström, P Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry |
topic_facet |
Animals Central Nervous System Cobalt Enkephalins Horseradish Peroxidase Immunohistochemistry In Situ Hybridization Lysine Neurons Protein Precursors RNA Messenger Salmon Sensitivity and Specificity Journal Article Research Support Non-U.S. Gov't |
description |
The distribution of neurons expressing preproenkephalin (PPE) mRNA in the brain of the salmon was investigated by means of non-radioactive in situ hybridization, and directly compared with the distribution of enkephalin-immunoreactive (ENKir) neurons. This approach, utilized here for the first time in a non-mammalian vertebrate for the identification of neurons containing opioid peptides, permitted a detailed analysis of the distribution of putative enkephalinergic neurons in the salmon brain. Several cell groups containing neurons that express PPE mRNA also contain ENKir neurons. Such cell groups are located in the ventral telencephalic area, the nucleus of the rostral mesencephalic tegmentum and another nucleus immediately dorsal to it, the torus semicircularis, the valvula cerebelli and the corpus cerebelli. These cell groups consistently contain larger numbers of PPE mRNA expressing cells than ENKir ones. Some cell groups express PPE mRNA, but do not contain ENKir neurons. These cell groups are located in the dorsal telencephalic area, the inferior lobes of the hypothalamus, the pretectal area, the magnocellular superficial pretectal nucleus, the optic tectum, the oculomotor nucleus, the trochlear nucleus, the magnocellular vestibular nucleus, the secondary gustatory nucleus, the superior and medial reticular nuclei, the motor nucleus of the vagus and the ventral horn of the spinal cord. Moreover, some cell groups contain ENKir neurons, but no PPE mRNA expressing neurons. These cell groups are located in the ventromedial thalamic nucleus, the lateral tuberal nucleus, the nucleus of the lateral recess and the nucleus of the posterior recess. The majority of these periventricular ENKir neurons were of the cerebrospinal fluid-contacting type. ENKir neurons were also located in the dorsal lateral tegmental nucleus and in area B9. The results also permitted a tentative identification of enkephalinergic neurons afferent to the optic tectum, that have previously not been identified with immunocytochemistry, located ... |
format |
Article in Journal/Newspaper |
author |
Vecino, E Perez, M T Ekström, P |
author_facet |
Vecino, E Perez, M T Ekström, P |
author_sort |
Vecino, E |
title |
Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry |
title_short |
Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry |
title_full |
Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry |
title_fullStr |
Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry |
title_full_unstemmed |
Localization of enkephalinergic neurons in the central nervous system of the salmon (Salmo salar L.) by in situ hybridization and immunocytochemistry |
title_sort |
localization of enkephalinergic neurons in the central nervous system of the salmon (salmo salar l.) by in situ hybridization and immunocytochemistry |
publisher |
Elsevier |
publishDate |
1995 |
url |
https://lup.lub.lu.se/record/7e6676dd-1786-4a04-aadf-9979983027aa https://doi.org/10.1016/0891-0618(95)00068-I |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Journal of Chemical Neuroanatomy; 9(2), pp 81-97 (1995) ISSN: 0891-0618 |
op_relation |
https://lup.lub.lu.se/record/7e6676dd-1786-4a04-aadf-9979983027aa http://dx.doi.org/10.1016/0891-0618(95)00068-I pmid:8561952 scopus:0029151344 |
op_doi |
https://doi.org/10.1016/0891-0618(95)00068-I |
container_title |
Journal of Chemical Neuroanatomy |
container_volume |
9 |
container_issue |
2 |
container_start_page |
81 |
op_container_end_page |
97 |
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1797568712168964096 |