Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies
Abstract in UndeterminedThe sedimentary ancient DNA (sedaDNA) technique offers a potentially invaluable means of investigating species evolution and extinction dynamics in high-latitude environments. An implicit assumption of the sedaDNA approach is that the extracted DNA is autochthonous with the h...
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ftulundlup:oai:lup.lub.lu.se:76feabd2-1973-4c45-9e06-cc44102e4468 2023-05-15T18:31:16+02:00 Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies Arnold, L.J. Roberts, R.G. MacPhee, R.D.E. Haile, J.S. Brock, J.S. Möller, Per Froese, D.G. Tikhonov, A.N. Chivas, A.R. Gilbert, T.P. Willerslev, E. 2011 https://lup.lub.lu.se/record/1540355 https://doi.org/10.1111/j.1502-3885.2010.00181.x eng eng John Wiley & Sons Inc. https://lup.lub.lu.se/record/1540355 http://dx.doi.org/10.1111/j.1502-3885.2010.00181.x wos:000292106800003 scopus:79959541010 Boreas; 40(3), pp 417-445 (2011) ISSN: 1502-3885 Geology contributiontojournal/article info:eu-repo/semantics/article text 2011 ftulundlup https://doi.org/10.1111/j.1502-3885.2010.00181.x 2023-02-01T23:29:25Z Abstract in UndeterminedThe sedimentary ancient DNA (sedaDNA) technique offers a potentially invaluable means of investigating species evolution and extinction dynamics in high-latitude environments. An implicit assumption of the sedaDNA approach is that the extracted DNA is autochthonous with the host deposit and that it has not been physically transported from older source deposits or reworked within the sedimentary profile by postdepositional mixing. In this paper we investigate whether these fundamental conditions are upheld at seven perennially frozen wetland sites across the Taimyr Peninsula and coastal lowlands of north-central Siberia. Optically stimulated luminescence (OSL) and radiocarbon (C-14) dating are used to constrain the ages of both the inorganic and organic fractions of perennially frozen deposits from which sedaDNA of extinct and extant species have been recovered. OSL and C-14 age/depth profiles, as well as single-grain equivalent dose (De) distribution characteristics, are used to assess the stratigraphic integrity of these sedaDNA sequences by (i) identifying the presence of primary or reworked organic and inorganic material, and (ii) examining the types of depositional and postdepositional processes that have affected specific sedimentary facies. The results of this study demonstrate that even though DNA preservation and stratigraphic integrity are commonly superior in perennially frozen settings, this does not, in itself, guarantee the suitability of the sedaDNA approach. The combined OSL and C-14 chronologies reveal that certain perennially frozen sites may be poorly suited for sedaDNA analysis, and that careful site selection is paramount to ensuring the accuracy of any sedaDNA study - particularly for 'latest appearance date' estimates of extinct taxa. Article in Journal/Newspaper Taimyr Siberia Lund University Publications (LUP) Boreas 40 3 417 445 |
institution |
Open Polar |
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Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Geology |
spellingShingle |
Geology Arnold, L.J. Roberts, R.G. MacPhee, R.D.E. Haile, J.S. Brock, J.S. Möller, Per Froese, D.G. Tikhonov, A.N. Chivas, A.R. Gilbert, T.P. Willerslev, E. Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies |
topic_facet |
Geology |
description |
Abstract in UndeterminedThe sedimentary ancient DNA (sedaDNA) technique offers a potentially invaluable means of investigating species evolution and extinction dynamics in high-latitude environments. An implicit assumption of the sedaDNA approach is that the extracted DNA is autochthonous with the host deposit and that it has not been physically transported from older source deposits or reworked within the sedimentary profile by postdepositional mixing. In this paper we investigate whether these fundamental conditions are upheld at seven perennially frozen wetland sites across the Taimyr Peninsula and coastal lowlands of north-central Siberia. Optically stimulated luminescence (OSL) and radiocarbon (C-14) dating are used to constrain the ages of both the inorganic and organic fractions of perennially frozen deposits from which sedaDNA of extinct and extant species have been recovered. OSL and C-14 age/depth profiles, as well as single-grain equivalent dose (De) distribution characteristics, are used to assess the stratigraphic integrity of these sedaDNA sequences by (i) identifying the presence of primary or reworked organic and inorganic material, and (ii) examining the types of depositional and postdepositional processes that have affected specific sedimentary facies. The results of this study demonstrate that even though DNA preservation and stratigraphic integrity are commonly superior in perennially frozen settings, this does not, in itself, guarantee the suitability of the sedaDNA approach. The combined OSL and C-14 chronologies reveal that certain perennially frozen sites may be poorly suited for sedaDNA analysis, and that careful site selection is paramount to ensuring the accuracy of any sedaDNA study - particularly for 'latest appearance date' estimates of extinct taxa. |
format |
Article in Journal/Newspaper |
author |
Arnold, L.J. Roberts, R.G. MacPhee, R.D.E. Haile, J.S. Brock, J.S. Möller, Per Froese, D.G. Tikhonov, A.N. Chivas, A.R. Gilbert, T.P. Willerslev, E. |
author_facet |
Arnold, L.J. Roberts, R.G. MacPhee, R.D.E. Haile, J.S. Brock, J.S. Möller, Per Froese, D.G. Tikhonov, A.N. Chivas, A.R. Gilbert, T.P. Willerslev, E. |
author_sort |
Arnold, L.J. |
title |
Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies |
title_short |
Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies |
title_full |
Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies |
title_fullStr |
Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies |
title_full_unstemmed |
Paper II - Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially-frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies |
title_sort |
paper ii - dirt, dates and dna: osl and radiocarbon chronologies of perennially-frozen sediments in siberia, and their implications for sedimentary ancient dna studies |
publisher |
John Wiley & Sons Inc. |
publishDate |
2011 |
url |
https://lup.lub.lu.se/record/1540355 https://doi.org/10.1111/j.1502-3885.2010.00181.x |
genre |
Taimyr Siberia |
genre_facet |
Taimyr Siberia |
op_source |
Boreas; 40(3), pp 417-445 (2011) ISSN: 1502-3885 |
op_relation |
https://lup.lub.lu.se/record/1540355 http://dx.doi.org/10.1111/j.1502-3885.2010.00181.x wos:000292106800003 scopus:79959541010 |
op_doi |
https://doi.org/10.1111/j.1502-3885.2010.00181.x |
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Boreas |
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40 |
container_issue |
3 |
container_start_page |
417 |
op_container_end_page |
445 |
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1766215004873293824 |