The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa.
Candida antarctica lipase B (CALB) and Thermomyces lanuginosa lipase (TLL) were evaluated as catalysts in different reaction media using hydrolysis of tributyrin as model reaction. In o/w emulsions, the enzymes were used in the free form and for use in monophasic organic media, the lipases were adso...
Published in: | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics |
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ftulundlup:oai:lup.lub.lu.se:7264a905-bbc6-4e43-bcdb-6dcfee77c157 2023-05-15T13:42:41+02:00 The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. Salis, A Svensson, Ingemar Monduzzi, M Solinas, V Adlercreutz, Patrick 2003 https://lup.lub.lu.se/record/129257 https://doi.org/10.1016/S1570-9639(02)00556-3 eng eng Elsevier https://lup.lub.lu.se/record/129257 http://dx.doi.org/10.1016/S1570-9639(02)00556-3 wos:000181783400016 pmid:12637021 scopus:0042121514 Biochimica et Biophysica Acta - Proteins and Proteomics; 1646(1-2), pp 145-151 (2003) ISSN: 1570-9639 Industrial Biotechnology Lipase Specific activity Tributyrin hydrolysis Lid contributiontojournal/article info:eu-repo/semantics/article text 2003 ftulundlup https://doi.org/10.1016/S1570-9639(02)00556-3 2023-02-01T23:27:17Z Candida antarctica lipase B (CALB) and Thermomyces lanuginosa lipase (TLL) were evaluated as catalysts in different reaction media using hydrolysis of tributyrin as model reaction. In o/w emulsions, the enzymes were used in the free form and for use in monophasic organic media, the lipases were adsorbed on porous polypropylene (Accurel EP-100). In monophasic organic media, the highest specific activity of both lipases was obtained in pure tributyrin at a water activity of >0.5 and at an enzyme loading of 10 mg/g support. With tributyrin emulsified in water, the specific activities were 2780 mol min−1 mg−1 for TLL and 535 mol min−1 mg−1 for CALB. Under optimal conditions in pure tributyrin, CALB expressed 49% of the activity in emulsion (264 mol min−1 mg−1) while TLL expressed only 9.2% (256 mol min−1 mg−1) of its activity in emulsion. This large decrease is probably due to the structure of TLL, which is a typical lipase with a large lid domain. Conversion between open and closed conformers of TLL involves large internal movements and catalysis probably requires more protein mobility in TLL than in CALB, which does not have a typical lid region. Furthermore, TLL lost more activity than CALB when the water activity was reduced below 0.5, which could be due to further reduction in protein mobility. Article in Journal/Newspaper Antarc* Antarctica Lund University Publications (LUP) Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1646 1-2 145 151 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Industrial Biotechnology Lipase Specific activity Tributyrin hydrolysis Lid |
spellingShingle |
Industrial Biotechnology Lipase Specific activity Tributyrin hydrolysis Lid Salis, A Svensson, Ingemar Monduzzi, M Solinas, V Adlercreutz, Patrick The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. |
topic_facet |
Industrial Biotechnology Lipase Specific activity Tributyrin hydrolysis Lid |
description |
Candida antarctica lipase B (CALB) and Thermomyces lanuginosa lipase (TLL) were evaluated as catalysts in different reaction media using hydrolysis of tributyrin as model reaction. In o/w emulsions, the enzymes were used in the free form and for use in monophasic organic media, the lipases were adsorbed on porous polypropylene (Accurel EP-100). In monophasic organic media, the highest specific activity of both lipases was obtained in pure tributyrin at a water activity of >0.5 and at an enzyme loading of 10 mg/g support. With tributyrin emulsified in water, the specific activities were 2780 mol min−1 mg−1 for TLL and 535 mol min−1 mg−1 for CALB. Under optimal conditions in pure tributyrin, CALB expressed 49% of the activity in emulsion (264 mol min−1 mg−1) while TLL expressed only 9.2% (256 mol min−1 mg−1) of its activity in emulsion. This large decrease is probably due to the structure of TLL, which is a typical lipase with a large lid domain. Conversion between open and closed conformers of TLL involves large internal movements and catalysis probably requires more protein mobility in TLL than in CALB, which does not have a typical lid region. Furthermore, TLL lost more activity than CALB when the water activity was reduced below 0.5, which could be due to further reduction in protein mobility. |
format |
Article in Journal/Newspaper |
author |
Salis, A Svensson, Ingemar Monduzzi, M Solinas, V Adlercreutz, Patrick |
author_facet |
Salis, A Svensson, Ingemar Monduzzi, M Solinas, V Adlercreutz, Patrick |
author_sort |
Salis, A |
title |
The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. |
title_short |
The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. |
title_full |
The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. |
title_fullStr |
The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. |
title_full_unstemmed |
The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. |
title_sort |
atypical lipase b from candida antarctica is better adapted for organic media than the typical lipase from thermomyces lanuginosa. |
publisher |
Elsevier |
publishDate |
2003 |
url |
https://lup.lub.lu.se/record/129257 https://doi.org/10.1016/S1570-9639(02)00556-3 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biochimica et Biophysica Acta - Proteins and Proteomics; 1646(1-2), pp 145-151 (2003) ISSN: 1570-9639 |
op_relation |
https://lup.lub.lu.se/record/129257 http://dx.doi.org/10.1016/S1570-9639(02)00556-3 wos:000181783400016 pmid:12637021 scopus:0042121514 |
op_doi |
https://doi.org/10.1016/S1570-9639(02)00556-3 |
container_title |
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics |
container_volume |
1646 |
container_issue |
1-2 |
container_start_page |
145 |
op_container_end_page |
151 |
_version_ |
1766171826303533056 |