Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters

Interest in sustainable development has increased throughout society during the past decades, and the chemical industry is no exception. The appeal for renewable raw materials has consequently increased and has paved the way for the implementation of industrial biotechnology. Since enzymes are used...

Full description

Bibliographic Details
Main Author: Hagström, Anna
Format: Doctoral or Postdoctoral Thesis
Language:English
Published: Department of Chemistry, Lund University 2010
Subjects:
Online Access:https://lup.lub.lu.se/record/1599270
id ftulundlup:oai:lup.lub.lu.se:6ece5149-f922-4fc7-854f-edcd50178f76
record_format openpolar
spelling ftulundlup:oai:lup.lub.lu.se:6ece5149-f922-4fc7-854f-edcd50178f76 2023-05-15T13:40:21+02:00 Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters Hagström, Anna 2010 application/pdf https://lup.lub.lu.se/record/1599270 eng eng Department of Chemistry, Lund University https://lup.lub.lu.se/record/1599270 urn:isbn:978-91-89627-65-9 info:eu-repo/semantics/restrictedAccess Industrial Biotechnology thesis/doccomp info:eu-repo/semantics/doctoralThesis text 2010 ftulundlup 2023-02-01T23:30:30Z Interest in sustainable development has increased throughout society during the past decades, and the chemical industry is no exception. The appeal for renewable raw materials has consequently increased and has paved the way for the implementation of industrial biotechnology. Since enzymes are used to convert biomass in nature, they may also be a good choice in the chemical industry. However, to identify if the use of enzymes is the best alternative for a particular application they must be evaluated. Investigations can be carried out on the enzyme itself, the reaction, the reactor design or the complete process. Analyses of the environmental impact and the economics of the process are also important. The focus of the interdisciplinary research programme Greenchem at Lund University is to develop speciality chemicals from renewable raw materials using enzymes as catalysts. The work presented in this thesis was focused on the reactor and the process, rather than the catalyst itself, or a specific reaction, as those have been covered in other projects within Greenchem. Lipases are among the most extensively studied enzymes. Candida antarctica lipase B (CalB), which can be used to catalyse several different reactions, is one example. This enzyme was most frequently used in the work described in this thesis, usually in an immobilised preparation, Novozym® 435 (N435). In the initial study presented in this thesis, four wax esters were produced and tested regarding their applicability as wood coatings. Unfortunately, even the best of these waxes showed too low resistance to grease stains. Towards the end of this work a new study was initiated; acrylation of polymers to be used as the main component in lacquers, with the hope of achieving better resistance than that achieved with the wax esters. These acrylates were produced on litre-scale via lipase-catalysed trans-esterification between ethyl acrylate and polyesters and polyethers, respectively, and were found to be comparable to the commercial alternatives available ... Doctoral or Postdoctoral Thesis Antarc* Antarctica Lund University Publications (LUP)
institution Open Polar
collection Lund University Publications (LUP)
op_collection_id ftulundlup
language English
topic Industrial Biotechnology
spellingShingle Industrial Biotechnology
Hagström, Anna
Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters
topic_facet Industrial Biotechnology
description Interest in sustainable development has increased throughout society during the past decades, and the chemical industry is no exception. The appeal for renewable raw materials has consequently increased and has paved the way for the implementation of industrial biotechnology. Since enzymes are used to convert biomass in nature, they may also be a good choice in the chemical industry. However, to identify if the use of enzymes is the best alternative for a particular application they must be evaluated. Investigations can be carried out on the enzyme itself, the reaction, the reactor design or the complete process. Analyses of the environmental impact and the economics of the process are also important. The focus of the interdisciplinary research programme Greenchem at Lund University is to develop speciality chemicals from renewable raw materials using enzymes as catalysts. The work presented in this thesis was focused on the reactor and the process, rather than the catalyst itself, or a specific reaction, as those have been covered in other projects within Greenchem. Lipases are among the most extensively studied enzymes. Candida antarctica lipase B (CalB), which can be used to catalyse several different reactions, is one example. This enzyme was most frequently used in the work described in this thesis, usually in an immobilised preparation, Novozym® 435 (N435). In the initial study presented in this thesis, four wax esters were produced and tested regarding their applicability as wood coatings. Unfortunately, even the best of these waxes showed too low resistance to grease stains. Towards the end of this work a new study was initiated; acrylation of polymers to be used as the main component in lacquers, with the hope of achieving better resistance than that achieved with the wax esters. These acrylates were produced on litre-scale via lipase-catalysed trans-esterification between ethyl acrylate and polyesters and polyethers, respectively, and were found to be comparable to the commercial alternatives available ...
format Doctoral or Postdoctoral Thesis
author Hagström, Anna
author_facet Hagström, Anna
author_sort Hagström, Anna
title Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters
title_short Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters
title_full Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters
title_fullStr Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters
title_full_unstemmed Improving Productivity and Enzyme Stability Through Process Design: Lipase-catalysed Synthesis of Epoxides and Esters
title_sort improving productivity and enzyme stability through process design: lipase-catalysed synthesis of epoxides and esters
publisher Department of Chemistry, Lund University
publishDate 2010
url https://lup.lub.lu.se/record/1599270
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation https://lup.lub.lu.se/record/1599270
urn:isbn:978-91-89627-65-9
op_rights info:eu-repo/semantics/restrictedAccess
_version_ 1766132680443822080