Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations
Persistent organochlorine pollutants (POPs) are suspected to interfere with hormone activity and the normal homeostasis of spermatogenesis. We investigated the relationships between sperm DNA fragmentation, apoptotic markers identified on ejaculated spermatozoa and POP levels in the blood of 652 adu...
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Language: | English |
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BioScientifica
2006
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Online Access: | https://lup.lub.lu.se/record/677645 https://doi.org/10.1530/rep.1.01034 |
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ftulundlup:oai:lup.lub.lu.se:6a30ce96-a046-46a5-bff2-6c41d882b03e 2023-05-15T16:30:01+02:00 Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations Stronati, A. Manicardi, G. C. Cecati, M. Bordicchia, M. Ferrante, L. Spano, M. Toft, G. Bonde, J. P. Jönsson, Bo A Rignell-Hydbom, Anna Rylander, Lars Giwercman, Aleksander Pedersen, H. S. Bonefeld-Jorgensen, E. C. Ludwicki, J. K. Lesovoy, V. Sakkas, D. Bizzaro, D. 2006 https://lup.lub.lu.se/record/677645 https://doi.org/10.1530/rep.1.01034 eng eng BioScientifica https://lup.lub.lu.se/record/677645 http://dx.doi.org/10.1530/rep.1.01034 wos:000243309300015 scopus:33846019604 Reproduction; 132(6), pp 949-958 (2006) ISSN: 1470-1626 Environmental Health and Occupational Health Obstetrics Gynecology and Reproductive Medicine contributiontojournal/article info:eu-repo/semantics/article text 2006 ftulundlup https://doi.org/10.1530/rep.1.01034 2023-02-01T23:27:11Z Persistent organochlorine pollutants (POPs) are suspected to interfere with hormone activity and the normal homeostasis of spermatogenesis. We investigated the relationships between sperm DNA fragmentation, apoptotic markers identified on ejaculated spermatozoa and POP levels in the blood of 652 adult males (200 Inuits from Greenland, 166 Swedish, 134 Polish and 152 Ukrainian). Serum levels of 2, 2', 4, 4', 5, 5'-hexachlorobiphenyl (CB-153), as a proxy of the total POP burden, and of 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE), as a proxy of the total DDT exposure were determined. Sperm DNA fragmentation was measured by using the TUNEL assay, whereas immunofluorescence methods were utilized for detecting proapoptotic (Fas) and anti-apoptotic (Bcl-xL) markers. Both TUNEL assay and apoptotic markers were statistically differed across the four populations. No correlation between neither sperm DNA fragmentation nor apoptotic sperm parameters and the large variations in POPs exposure was observed for the separate study groups. However, considering the European populations taken together, we showed that both %TUNEL positivity and Bcl-xL were related to CB-153 serum levels, whereas our study failed to demonstrate any relations between DDE and %TUNEL positivity and apoptotic sperm biomarkers (Fas and Bcl-xL) in any region or overall regions. These results suggest that CB-153 and related chemicals might alter sperm DNA integrity and Bcl-xL levels in European adult males, but not in the highly exposed Inuit men. Additional issues (genetic background, lifestyle habits and characterization of total xeno-hormonal activities) need to he investigated in order to fully assess the population variations observed. Article in Journal/Newspaper Greenland inuit inuits Lund University Publications (LUP) Greenland Reproduction 132 6 949 958 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Environmental Health and Occupational Health Obstetrics Gynecology and Reproductive Medicine |
spellingShingle |
Environmental Health and Occupational Health Obstetrics Gynecology and Reproductive Medicine Stronati, A. Manicardi, G. C. Cecati, M. Bordicchia, M. Ferrante, L. Spano, M. Toft, G. Bonde, J. P. Jönsson, Bo A Rignell-Hydbom, Anna Rylander, Lars Giwercman, Aleksander Pedersen, H. S. Bonefeld-Jorgensen, E. C. Ludwicki, J. K. Lesovoy, V. Sakkas, D. Bizzaro, D. Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations |
topic_facet |
Environmental Health and Occupational Health Obstetrics Gynecology and Reproductive Medicine |
description |
Persistent organochlorine pollutants (POPs) are suspected to interfere with hormone activity and the normal homeostasis of spermatogenesis. We investigated the relationships between sperm DNA fragmentation, apoptotic markers identified on ejaculated spermatozoa and POP levels in the blood of 652 adult males (200 Inuits from Greenland, 166 Swedish, 134 Polish and 152 Ukrainian). Serum levels of 2, 2', 4, 4', 5, 5'-hexachlorobiphenyl (CB-153), as a proxy of the total POP burden, and of 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE), as a proxy of the total DDT exposure were determined. Sperm DNA fragmentation was measured by using the TUNEL assay, whereas immunofluorescence methods were utilized for detecting proapoptotic (Fas) and anti-apoptotic (Bcl-xL) markers. Both TUNEL assay and apoptotic markers were statistically differed across the four populations. No correlation between neither sperm DNA fragmentation nor apoptotic sperm parameters and the large variations in POPs exposure was observed for the separate study groups. However, considering the European populations taken together, we showed that both %TUNEL positivity and Bcl-xL were related to CB-153 serum levels, whereas our study failed to demonstrate any relations between DDE and %TUNEL positivity and apoptotic sperm biomarkers (Fas and Bcl-xL) in any region or overall regions. These results suggest that CB-153 and related chemicals might alter sperm DNA integrity and Bcl-xL levels in European adult males, but not in the highly exposed Inuit men. Additional issues (genetic background, lifestyle habits and characterization of total xeno-hormonal activities) need to he investigated in order to fully assess the population variations observed. |
format |
Article in Journal/Newspaper |
author |
Stronati, A. Manicardi, G. C. Cecati, M. Bordicchia, M. Ferrante, L. Spano, M. Toft, G. Bonde, J. P. Jönsson, Bo A Rignell-Hydbom, Anna Rylander, Lars Giwercman, Aleksander Pedersen, H. S. Bonefeld-Jorgensen, E. C. Ludwicki, J. K. Lesovoy, V. Sakkas, D. Bizzaro, D. |
author_facet |
Stronati, A. Manicardi, G. C. Cecati, M. Bordicchia, M. Ferrante, L. Spano, M. Toft, G. Bonde, J. P. Jönsson, Bo A Rignell-Hydbom, Anna Rylander, Lars Giwercman, Aleksander Pedersen, H. S. Bonefeld-Jorgensen, E. C. Ludwicki, J. K. Lesovoy, V. Sakkas, D. Bizzaro, D. |
author_sort |
Stronati, A. |
title |
Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations |
title_short |
Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations |
title_full |
Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations |
title_fullStr |
Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations |
title_full_unstemmed |
Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p '-DDE in European and Inuit populations |
title_sort |
relationships between sperm dna fragmentation, sperm apoptotic markers and serum levels of cb-153 and p,p '-dde in european and inuit populations |
publisher |
BioScientifica |
publishDate |
2006 |
url |
https://lup.lub.lu.se/record/677645 https://doi.org/10.1530/rep.1.01034 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland inuit inuits |
genre_facet |
Greenland inuit inuits |
op_source |
Reproduction; 132(6), pp 949-958 (2006) ISSN: 1470-1626 |
op_relation |
https://lup.lub.lu.se/record/677645 http://dx.doi.org/10.1530/rep.1.01034 wos:000243309300015 scopus:33846019604 |
op_doi |
https://doi.org/10.1530/rep.1.01034 |
container_title |
Reproduction |
container_volume |
132 |
container_issue |
6 |
container_start_page |
949 |
op_container_end_page |
958 |
_version_ |
1766019740615049216 |