Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.)
Aryl hydrocarbon receptor (AhR) genes encode proteins involved in mediating the toxic responses induced by several environmental pollutants. Here, we describe the identification of the first two AhR1 (alpha and beta) genes and two additional AhR2 (alpha and beta) genes in the tetraploid species Atla...
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ftulundlup:oai:lup.lub.lu.se:3df2c0b1-4bcd-47f8-a97e-0abae4ed08cc 2023-05-15T15:30:16+02:00 Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn 2004 https://lup.lub.lu.se/record/136976 https://doi.org/10.1016/j.aquatox.2004.02.006 eng eng Elsevier https://lup.lub.lu.se/record/136976 http://dx.doi.org/10.1016/j.aquatox.2004.02.006 wos:000222066000002 pmid:15159049 scopus:2542469651 Aquatic Toxicology; 68(3), pp 219-232 (2004) ISSN: 1879-1514 Ecology Biological Sciences contributiontojournal/article info:eu-repo/semantics/article text 2004 ftulundlup https://doi.org/10.1016/j.aquatox.2004.02.006 2023-02-01T23:33:01Z Aryl hydrocarbon receptor (AhR) genes encode proteins involved in mediating the toxic responses induced by several environmental pollutants. Here, we describe the identification of the first two AhR1 (alpha and beta) genes and two additional AhR2 (alpha and beta) genes in the tetraploid species Atlantic salmon (Salmo salar L.) from a cosmid library screening. Cosmid clones containing genomic salmon AhR sequences were isolated using a cDNA clone containing the coding region of the Atlantic salmon AhR2gamma as a probe. Screening revealed 14 positive clones, from which four were chosen for further analyses. One of the cosmids contained genomic AhR sequences that were highly similar to the rainbow trout (Oncorhynchus mykiss) AhR2alpha and beta genes. SMART RACE amplified two complete, highly similar but not identical AhR type 2 sequences from salmon cDNA, which from phylogenetic analyses were determined as the rainbow trout AhR2alpha and beta orthologs. The salmon AhR2alpha and beta encode proteins of 1071 and 1058 residues, respectively, and encompass characteristic AhR sequence elements like a basic-helix-loop-helix (bHLH) and two PER-ARNT-SIM (PAS) domains. Both genes are transcribed in liver, spleen and muscle tissues of adult salmon. A second cosmid contained partial sequences, which were identical to the previously characterized AhR2gamma gene. The last two cosmids contained partial genomic AhR sequences, which were more similar to other AhR type 1 fish genes than the four characterized salmon AhR2 genes. However, attempts to amplify the corresponding complete cDNA sequences of the inserts proved very difficult, suggesting that these genes are non-functional or very weakly transcribed in the examined tissues. Phylogenetic analyses of the conserved regions did, however, clearly indicate that these two AhRs belong to the AhR type 1 clade and have been assigned as the Atlantic salmon AhR1alpha and AhR1beta genes. Taken together, these findings demonstrate that multiple AhR genes are present in Atlantic salmon ... Article in Journal/Newspaper Atlantic salmon Salmo salar Lund University Publications (LUP) Aquatic Toxicology 68 3 219 232 |
institution |
Open Polar |
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Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Ecology Biological Sciences |
spellingShingle |
Ecology Biological Sciences Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) |
topic_facet |
Ecology Biological Sciences |
description |
Aryl hydrocarbon receptor (AhR) genes encode proteins involved in mediating the toxic responses induced by several environmental pollutants. Here, we describe the identification of the first two AhR1 (alpha and beta) genes and two additional AhR2 (alpha and beta) genes in the tetraploid species Atlantic salmon (Salmo salar L.) from a cosmid library screening. Cosmid clones containing genomic salmon AhR sequences were isolated using a cDNA clone containing the coding region of the Atlantic salmon AhR2gamma as a probe. Screening revealed 14 positive clones, from which four were chosen for further analyses. One of the cosmids contained genomic AhR sequences that were highly similar to the rainbow trout (Oncorhynchus mykiss) AhR2alpha and beta genes. SMART RACE amplified two complete, highly similar but not identical AhR type 2 sequences from salmon cDNA, which from phylogenetic analyses were determined as the rainbow trout AhR2alpha and beta orthologs. The salmon AhR2alpha and beta encode proteins of 1071 and 1058 residues, respectively, and encompass characteristic AhR sequence elements like a basic-helix-loop-helix (bHLH) and two PER-ARNT-SIM (PAS) domains. Both genes are transcribed in liver, spleen and muscle tissues of adult salmon. A second cosmid contained partial sequences, which were identical to the previously characterized AhR2gamma gene. The last two cosmids contained partial genomic AhR sequences, which were more similar to other AhR type 1 fish genes than the four characterized salmon AhR2 genes. However, attempts to amplify the corresponding complete cDNA sequences of the inserts proved very difficult, suggesting that these genes are non-functional or very weakly transcribed in the examined tissues. Phylogenetic analyses of the conserved regions did, however, clearly indicate that these two AhRs belong to the AhR type 1 clade and have been assigned as the Atlantic salmon AhR1alpha and AhR1beta genes. Taken together, these findings demonstrate that multiple AhR genes are present in Atlantic salmon ... |
format |
Article in Journal/Newspaper |
author |
Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn |
author_facet |
Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn |
author_sort |
Hansson, Maria |
title |
Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) |
title_short |
Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) |
title_full |
Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) |
title_fullStr |
Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Unprecedented genomic diversity of AhR1 and AhR2 genes in Atlantic salmon (Salmo salar L.) |
title_sort |
unprecedented genomic diversity of ahr1 and ahr2 genes in atlantic salmon (salmo salar l.) |
publisher |
Elsevier |
publishDate |
2004 |
url |
https://lup.lub.lu.se/record/136976 https://doi.org/10.1016/j.aquatox.2004.02.006 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquatic Toxicology; 68(3), pp 219-232 (2004) ISSN: 1879-1514 |
op_relation |
https://lup.lub.lu.se/record/136976 http://dx.doi.org/10.1016/j.aquatox.2004.02.006 wos:000222066000002 pmid:15159049 scopus:2542469651 |
op_doi |
https://doi.org/10.1016/j.aquatox.2004.02.006 |
container_title |
Aquatic Toxicology |
container_volume |
68 |
container_issue |
3 |
container_start_page |
219 |
op_container_end_page |
232 |
_version_ |
1766360705542389760 |