Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution
The assimilation of inorganic compounds in foraminiferal metabolism compared to predation or organic matter assimilation is unknown. Here, we investigate possible inorganic-compound assimilation in Nonionellina labradorica, a common kleptoplastidic benthic foraminifer from Arctic and North Atlantic...
Published in: | Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Wiley-Blackwell
2019
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Online Access: | https://lup.lub.lu.se/record/18cabc60-c21d-4c22-95c8-5cbe40daaba8 https://doi.org/10.1111/1462-2920.14433 |
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ftulundlup:oai:lup.lub.lu.se:18cabc60-c21d-4c22-95c8-5cbe40daaba8 2024-02-11T10:01:28+01:00 Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution Jauffrais, Thierry LeKieffre, Charlotte Schweizer, Magali Geslin, Emmanuelle Metzger, Edouard Bernhard, Joan M. Jesus, Bruno Filipsson, Helena L. Maire, Olivier Meibom, Anders 2019 https://lup.lub.lu.se/record/18cabc60-c21d-4c22-95c8-5cbe40daaba8 https://doi.org/10.1111/1462-2920.14433 eng eng Wiley-Blackwell https://lup.lub.lu.se/record/18cabc60-c21d-4c22-95c8-5cbe40daaba8 http://dx.doi.org/10.1111/1462-2920.14433 scopus:85055527658 pmid:30277305 Environmental Microbiology; 21(1), pp 125-141 (2019) ISSN: 1462-2912 Oceanography Hydrology Water Resources Natural Sciences Biological Sciences contributiontojournal/article info:eu-repo/semantics/article text 2019 ftulundlup https://doi.org/10.1111/1462-2920.14433 2024-01-17T23:29:37Z The assimilation of inorganic compounds in foraminiferal metabolism compared to predation or organic matter assimilation is unknown. Here, we investigate possible inorganic-compound assimilation in Nonionellina labradorica, a common kleptoplastidic benthic foraminifer from Arctic and North Atlantic sublittoral regions. The objectives were to identify the source of the foraminiferal kleptoplasts, assess their photosynthetic functionality in light and darkness and investigate inorganic nitrogen and sulfate assimilation. We used DNA barcoding of a ~ 830 bp fragment from the SSU rDNA to identify the kleptoplasts and correlated transmission electron microscopy and nanometre-scale secondary ion mass spectrometry (TEM-NanoSIMS) isotopic imaging to study 13C-bicarbonate, 15N-ammonium and 34S-sulfate uptake. In addition, respiration rate measurements were determined to assess the response of N. labradorica to light. The DNA sequences established that over 80% of the kleptoplasts belonged to Thalassiosira (with 96%–99% identity), a cosmopolitan planktonic diatom. TEM-NanoSIMS imaging revealed degraded cytoplasm and an absence of 13C assimilation in foraminifera exposed to light. Oxygen measurements showed higher respiration rates under light than dark conditions, and no O2 production was detected. These results indicate that the photosynthetic pathways in N. labradorica are not functional. Furthermore, N. labradorica assimilated both 15N-ammonium and 34S-sulfate into its cytoplasm, which suggests that foraminifera might have several ammonium or sulfate assimilation pathways, involving either the kleptoplasts or bona fide foraminiferal pathway(s) not yet identified. Article in Journal/Newspaper Arctic Foraminifera* North Atlantic Lund University Publications (LUP) Arctic Environmental Microbiology 21 1 125 141 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Oceanography Hydrology Water Resources Natural Sciences Biological Sciences |
spellingShingle |
Oceanography Hydrology Water Resources Natural Sciences Biological Sciences Jauffrais, Thierry LeKieffre, Charlotte Schweizer, Magali Geslin, Emmanuelle Metzger, Edouard Bernhard, Joan M. Jesus, Bruno Filipsson, Helena L. Maire, Olivier Meibom, Anders Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution |
topic_facet |
Oceanography Hydrology Water Resources Natural Sciences Biological Sciences |
description |
The assimilation of inorganic compounds in foraminiferal metabolism compared to predation or organic matter assimilation is unknown. Here, we investigate possible inorganic-compound assimilation in Nonionellina labradorica, a common kleptoplastidic benthic foraminifer from Arctic and North Atlantic sublittoral regions. The objectives were to identify the source of the foraminiferal kleptoplasts, assess their photosynthetic functionality in light and darkness and investigate inorganic nitrogen and sulfate assimilation. We used DNA barcoding of a ~ 830 bp fragment from the SSU rDNA to identify the kleptoplasts and correlated transmission electron microscopy and nanometre-scale secondary ion mass spectrometry (TEM-NanoSIMS) isotopic imaging to study 13C-bicarbonate, 15N-ammonium and 34S-sulfate uptake. In addition, respiration rate measurements were determined to assess the response of N. labradorica to light. The DNA sequences established that over 80% of the kleptoplasts belonged to Thalassiosira (with 96%–99% identity), a cosmopolitan planktonic diatom. TEM-NanoSIMS imaging revealed degraded cytoplasm and an absence of 13C assimilation in foraminifera exposed to light. Oxygen measurements showed higher respiration rates under light than dark conditions, and no O2 production was detected. These results indicate that the photosynthetic pathways in N. labradorica are not functional. Furthermore, N. labradorica assimilated both 15N-ammonium and 34S-sulfate into its cytoplasm, which suggests that foraminifera might have several ammonium or sulfate assimilation pathways, involving either the kleptoplasts or bona fide foraminiferal pathway(s) not yet identified. |
format |
Article in Journal/Newspaper |
author |
Jauffrais, Thierry LeKieffre, Charlotte Schweizer, Magali Geslin, Emmanuelle Metzger, Edouard Bernhard, Joan M. Jesus, Bruno Filipsson, Helena L. Maire, Olivier Meibom, Anders |
author_facet |
Jauffrais, Thierry LeKieffre, Charlotte Schweizer, Magali Geslin, Emmanuelle Metzger, Edouard Bernhard, Joan M. Jesus, Bruno Filipsson, Helena L. Maire, Olivier Meibom, Anders |
author_sort |
Jauffrais, Thierry |
title |
Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution |
title_short |
Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution |
title_full |
Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution |
title_fullStr |
Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution |
title_full_unstemmed |
Kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic C, N and S studied with sub-cellular resolution |
title_sort |
kleptoplastidic benthic foraminifera from aphotic habitats : insights into assimilation of inorganic c, n and s studied with sub-cellular resolution |
publisher |
Wiley-Blackwell |
publishDate |
2019 |
url |
https://lup.lub.lu.se/record/18cabc60-c21d-4c22-95c8-5cbe40daaba8 https://doi.org/10.1111/1462-2920.14433 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Foraminifera* North Atlantic |
genre_facet |
Arctic Foraminifera* North Atlantic |
op_source |
Environmental Microbiology; 21(1), pp 125-141 (2019) ISSN: 1462-2912 |
op_relation |
https://lup.lub.lu.se/record/18cabc60-c21d-4c22-95c8-5cbe40daaba8 http://dx.doi.org/10.1111/1462-2920.14433 scopus:85055527658 pmid:30277305 |
op_doi |
https://doi.org/10.1111/1462-2920.14433 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
1 |
container_start_page |
125 |
op_container_end_page |
141 |
_version_ |
1790597268844314624 |