Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals
Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of co...
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Online Access: | https://doi.org/10.1038/s41467-021-23216-7 https://ora.ox.ac.uk/objects/uuid:f67c1864-2151-458f-a541-b30f52d49b24 |
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ftuloxford:oai:ora.ox.ac.uk:uuid:f67c1864-2151-458f-a541-b30f52d49b24 2023-05-15T17:54:19+02:00 Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH 2021-04-20 https://doi.org/10.1038/s41467-021-23216-7 https://ora.ox.ac.uk/objects/uuid:f67c1864-2151-458f-a541-b30f52d49b24 eng eng Springer Nature doi:10.1038/s41467-021-23216-7 https://ora.ox.ac.uk/objects/uuid:f67c1864-2151-458f-a541-b30f52d49b24 https://doi.org/10.1038/s41467-021-23216-7 info:eu-repo/semantics/openAccess CC Attribution (CC BY) CC-BY Journal article 2021 ftuloxford https://doi.org/10.1038/s41467-021-23216-7 2022-06-28T20:28:15Z Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria. Article in Journal/Newspaper Pacific oyster ORA - Oxford University Research Archive Pacific Nature Communications 12 1 |
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Open Polar |
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ORA - Oxford University Research Archive |
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ftuloxford |
language |
English |
description |
Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria. |
format |
Article in Journal/Newspaper |
author |
Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH |
spellingShingle |
Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
author_facet |
Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH |
author_sort |
Xu, F |
title |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_short |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_full |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_fullStr |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_full_unstemmed |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_sort |
evidence from oyster suggests an ancient role for pdx in regulating insulin gene expression in animals |
publisher |
Springer Nature |
publishDate |
2021 |
url |
https://doi.org/10.1038/s41467-021-23216-7 https://ora.ox.ac.uk/objects/uuid:f67c1864-2151-458f-a541-b30f52d49b24 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
doi:10.1038/s41467-021-23216-7 https://ora.ox.ac.uk/objects/uuid:f67c1864-2151-458f-a541-b30f52d49b24 https://doi.org/10.1038/s41467-021-23216-7 |
op_rights |
info:eu-repo/semantics/openAccess CC Attribution (CC BY) |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41467-021-23216-7 |
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Nature Communications |
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12 |
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1 |
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1766162059751325696 |