Characterisation of the human embryonic and foetal epicardium during heart development
The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epi...
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2016
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ftuloxford:oai:ora.ox.ac.uk:uuid:c19ae969-1e13-4f34-87a7-ef27c36ad764 2024-09-09T19:31:11+00:00 Characterisation of the human embryonic and foetal epicardium during heart development Risebro, CA Vieira, JM Klotz, L Riley, PR 2016-07-29 https://doi.org/10.1242/dev.127621 https://ora.ox.ac.uk/objects/uuid:c19ae969-1e13-4f34-87a7-ef27c36ad764 eng eng Company of Biologists doi:10.1242/dev.127621 https://ora.ox.ac.uk/objects/uuid:c19ae969-1e13-4f34-87a7-ef27c36ad764 https://doi.org/10.1242/dev.127621 info:eu-repo/semantics/openAccess Journal article 2016 ftuloxford https://doi.org/10.1242/dev.127621 2024-08-05T14:07:48Z The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair. Article in Journal/Newspaper Avian Studies ORA - Oxford University Research Archive Development |
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Open Polar |
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ORA - Oxford University Research Archive |
op_collection_id |
ftuloxford |
language |
English |
description |
The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair. |
format |
Article in Journal/Newspaper |
author |
Risebro, CA Vieira, JM Klotz, L Riley, PR |
spellingShingle |
Risebro, CA Vieira, JM Klotz, L Riley, PR Characterisation of the human embryonic and foetal epicardium during heart development |
author_facet |
Risebro, CA Vieira, JM Klotz, L Riley, PR |
author_sort |
Risebro, CA |
title |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_short |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_full |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_fullStr |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_full_unstemmed |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_sort |
characterisation of the human embryonic and foetal epicardium during heart development |
publisher |
Company of Biologists |
publishDate |
2016 |
url |
https://doi.org/10.1242/dev.127621 https://ora.ox.ac.uk/objects/uuid:c19ae969-1e13-4f34-87a7-ef27c36ad764 |
genre |
Avian Studies |
genre_facet |
Avian Studies |
op_relation |
doi:10.1242/dev.127621 https://ora.ox.ac.uk/objects/uuid:c19ae969-1e13-4f34-87a7-ef27c36ad764 https://doi.org/10.1242/dev.127621 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1242/dev.127621 |
container_title |
Development |
_version_ |
1809900088608161792 |