FGF4 independent derivation of trophoblast stem cells from the common vole.
The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated...
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Public Library of Science
2016
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Online Access: | https://doi.org/10.1371/journal.pone.0007161 https://ora.ox.ac.uk/objects/uuid:106544c7-cf88-4939-8824-1e48c28e57ae |
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ftuloxford:oai:ora.ox.ac.uk:uuid:106544c7-cf88-4939-8824-1e48c28e57ae 2023-05-15T15:56:34+02:00 FGF4 independent derivation of trophoblast stem cells from the common vole. Grigor'eva, E Shevchenko, A Mazurok, N Elisaphenko, E Zhelezova, A Shilov, A Dyban, P Dyban, A Noniashvili, E Slobodyanyuk, S Nesterova, T Brockdorff, N Zakian, S 2016-07-28 https://doi.org/10.1371/journal.pone.0007161 https://ora.ox.ac.uk/objects/uuid:106544c7-cf88-4939-8824-1e48c28e57ae eng eng Public Library of Science doi:10.1371/journal.pone.0007161 https://ora.ox.ac.uk/objects/uuid:106544c7-cf88-4939-8824-1e48c28e57ae https://doi.org/10.1371/journal.pone.0007161 info:eu-repo/semantics/openAccess CC Attribution (CC BY) CC-BY Journal article 2016 ftuloxford https://doi.org/10.1371/journal.pone.0007161 2022-06-28T20:06:03Z The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated state of multipotent TS cells. Here we report the derivation of stable TS-like cell lines from the vole M. rossiaemeridionalis, in the absence of FGF4 and heparin. Vole TS-like cells are similar to murine TS cells with respect to their morphology, transcription factor gene expression and differentiation in vitro into derivatives of the trophectoderm lineage, and with respect to their ability to invade and erode host tissues, forming haemorrhagic tumours after subcutaneous injection into nude mice. Moreover, vole TS-like cells carry an inactive paternal X chromosome, indicating that they have undergone imprinted X inactivation, which is characteristic of the trophoblast lineage. Our results indicate that an alternative signaling pathway may be responsible for the establishment and stable proliferation of vole TS-like cells. Article in Journal/Newspaper Common vole ORA - Oxford University Research Archive PLoS ONE 4 9 e7161 |
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Open Polar |
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ORA - Oxford University Research Archive |
op_collection_id |
ftuloxford |
language |
English |
description |
The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated state of multipotent TS cells. Here we report the derivation of stable TS-like cell lines from the vole M. rossiaemeridionalis, in the absence of FGF4 and heparin. Vole TS-like cells are similar to murine TS cells with respect to their morphology, transcription factor gene expression and differentiation in vitro into derivatives of the trophectoderm lineage, and with respect to their ability to invade and erode host tissues, forming haemorrhagic tumours after subcutaneous injection into nude mice. Moreover, vole TS-like cells carry an inactive paternal X chromosome, indicating that they have undergone imprinted X inactivation, which is characteristic of the trophoblast lineage. Our results indicate that an alternative signaling pathway may be responsible for the establishment and stable proliferation of vole TS-like cells. |
format |
Article in Journal/Newspaper |
author |
Grigor'eva, E Shevchenko, A Mazurok, N Elisaphenko, E Zhelezova, A Shilov, A Dyban, P Dyban, A Noniashvili, E Slobodyanyuk, S Nesterova, T Brockdorff, N Zakian, S |
spellingShingle |
Grigor'eva, E Shevchenko, A Mazurok, N Elisaphenko, E Zhelezova, A Shilov, A Dyban, P Dyban, A Noniashvili, E Slobodyanyuk, S Nesterova, T Brockdorff, N Zakian, S FGF4 independent derivation of trophoblast stem cells from the common vole. |
author_facet |
Grigor'eva, E Shevchenko, A Mazurok, N Elisaphenko, E Zhelezova, A Shilov, A Dyban, P Dyban, A Noniashvili, E Slobodyanyuk, S Nesterova, T Brockdorff, N Zakian, S |
author_sort |
Grigor'eva, E |
title |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_short |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_full |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_fullStr |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_full_unstemmed |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_sort |
fgf4 independent derivation of trophoblast stem cells from the common vole. |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0007161 https://ora.ox.ac.uk/objects/uuid:106544c7-cf88-4939-8824-1e48c28e57ae |
genre |
Common vole |
genre_facet |
Common vole |
op_relation |
doi:10.1371/journal.pone.0007161 https://ora.ox.ac.uk/objects/uuid:106544c7-cf88-4939-8824-1e48c28e57ae https://doi.org/10.1371/journal.pone.0007161 |
op_rights |
info:eu-repo/semantics/openAccess CC Attribution (CC BY) |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0007161 |
container_title |
PLoS ONE |
container_volume |
4 |
container_issue |
9 |
container_start_page |
e7161 |
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1766391965242359808 |