Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor
Diacylglycerols (DAG) have been widely used in many industries due to their remarkable capabilities as emulsifiers and stabilisers. However, developing a sustainable and an effective synthesis method for DAG remains a challenge. Continuous flow bio-reactor is recognized to be more productive, contro...
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ftulancaster:oai:eprints.lancs.ac.uk:190082 2023-08-27T04:06:23+02:00 Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor Abd Razak, Nurul Nadiah Cognet, Patrick Pérès, Yolande Gew, Lai Ti Aroua, Mohamed Kheireddine 2022-11-01 https://eprints.lancs.ac.uk/id/eprint/190082/ https://doi.org/10.1016/j.jclepro.2022.133816 unknown Abd Razak, Nurul Nadiah and Cognet, Patrick and Pérès, Yolande and Gew, Lai Ti and Aroua, Mohamed Kheireddine (2022) Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor. Journal of Cleaner Production, 373. ISSN 0959-6526 Journal Article PeerReviewed 2022 ftulancaster https://doi.org/10.1016/j.jclepro.2022.133816 2023-08-03T22:43:05Z Diacylglycerols (DAG) have been widely used in many industries due to their remarkable capabilities as emulsifiers and stabilisers. However, developing a sustainable and an effective synthesis method for DAG remains a challenge. Continuous flow bio-reactor is recognized to be more productive, controllable, and reliable instrument for developing green and intensified processes. In this work, a continuous flow packed bed millireactor was employed for the synthesis of glycerol dioelate (GDO) catalyzed by immobilized lipase namely Candida antartica. Experiments were carried out to evaluate the kinetic parameters as well as to assess the internal and external mass transfer limitations. Using one-factor-at-a-time variables method, maximum oleic acid conversion and GDO selectivity were achieved at 85% and 74% respectively, at 0.15 g of lipase, 77 min of residence time with 1.6:1 molar ratio of oleic acid/glycerol. Hydrodynamic studies showed that the esterification reaction is kinetically controlled and unaffected by external and internal mass transfer. Employing Lilly–Hornby model for kinetic evaluation, K m values increased with increasing flow rates, whereas, V max appeared to be flow rate independent. Reusability tests revealed that the activity of immobilized lipase remained the same after 9 successive reaction cycles. At 11 days of operation, the stability of the lipase in the continuous packed bed millireactor decreased only 5–7%, indicating satisfying operational results and recyclability. This work may promote the enzymatic engineering synthesis of DAG, facilitating the creation of a cleaner and safer process. It has the potential to broaden the application of enzymes in continuous flow micro or millireactors. Article in Journal/Newspaper antartic* Lancaster University: Lancaster Eprints Journal of Cleaner Production 373 133816 |
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Lancaster University: Lancaster Eprints |
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ftulancaster |
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description |
Diacylglycerols (DAG) have been widely used in many industries due to their remarkable capabilities as emulsifiers and stabilisers. However, developing a sustainable and an effective synthesis method for DAG remains a challenge. Continuous flow bio-reactor is recognized to be more productive, controllable, and reliable instrument for developing green and intensified processes. In this work, a continuous flow packed bed millireactor was employed for the synthesis of glycerol dioelate (GDO) catalyzed by immobilized lipase namely Candida antartica. Experiments were carried out to evaluate the kinetic parameters as well as to assess the internal and external mass transfer limitations. Using one-factor-at-a-time variables method, maximum oleic acid conversion and GDO selectivity were achieved at 85% and 74% respectively, at 0.15 g of lipase, 77 min of residence time with 1.6:1 molar ratio of oleic acid/glycerol. Hydrodynamic studies showed that the esterification reaction is kinetically controlled and unaffected by external and internal mass transfer. Employing Lilly–Hornby model for kinetic evaluation, K m values increased with increasing flow rates, whereas, V max appeared to be flow rate independent. Reusability tests revealed that the activity of immobilized lipase remained the same after 9 successive reaction cycles. At 11 days of operation, the stability of the lipase in the continuous packed bed millireactor decreased only 5–7%, indicating satisfying operational results and recyclability. This work may promote the enzymatic engineering synthesis of DAG, facilitating the creation of a cleaner and safer process. It has the potential to broaden the application of enzymes in continuous flow micro or millireactors. |
format |
Article in Journal/Newspaper |
author |
Abd Razak, Nurul Nadiah Cognet, Patrick Pérès, Yolande Gew, Lai Ti Aroua, Mohamed Kheireddine |
spellingShingle |
Abd Razak, Nurul Nadiah Cognet, Patrick Pérès, Yolande Gew, Lai Ti Aroua, Mohamed Kheireddine Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor |
author_facet |
Abd Razak, Nurul Nadiah Cognet, Patrick Pérès, Yolande Gew, Lai Ti Aroua, Mohamed Kheireddine |
author_sort |
Abd Razak, Nurul Nadiah |
title |
Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor |
title_short |
Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor |
title_full |
Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor |
title_fullStr |
Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor |
title_full_unstemmed |
Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor |
title_sort |
kinetics and hydrodynamics of candida antartica lipase-catalyzed synthesis of glycerol dioleate (gdo) in a continuous flow packed-bed millireactor |
publishDate |
2022 |
url |
https://eprints.lancs.ac.uk/id/eprint/190082/ https://doi.org/10.1016/j.jclepro.2022.133816 |
genre |
antartic* |
genre_facet |
antartic* |
op_relation |
Abd Razak, Nurul Nadiah and Cognet, Patrick and Pérès, Yolande and Gew, Lai Ti and Aroua, Mohamed Kheireddine (2022) Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor. Journal of Cleaner Production, 373. ISSN 0959-6526 |
op_doi |
https://doi.org/10.1016/j.jclepro.2022.133816 |
container_title |
Journal of Cleaner Production |
container_volume |
373 |
container_start_page |
133816 |
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1775347254498951168 |