Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas
The application of a simple, fast and reliable volumetric microrespirometric method to assess respiration of bivalve larvae is discussed. As a model, C. gigas larvae of various sizes were used. Metabolic activity of veliger larvae was assessed by measuring respiratory rate for use in ecophysiologica...
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Online Access: | https://pure.uhi.ac.uk/en/publications/f9e411ca-ab23-4135-a53c-d09805199b2c https://doi.org/10.1051/alr:2004018 |
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ftuhipublicatio:oai:pure.atira.dk:publications/f9e411ca-ab23-4135-a53c-d09805199b2c 2024-02-04T09:59:51+01:00 Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas Goulletquer, Philippe Wolowicz, M Latala, A Brown, Craig J Cragg, Simon 2004 https://pure.uhi.ac.uk/en/publications/f9e411ca-ab23-4135-a53c-d09805199b2c https://doi.org/10.1051/alr:2004018 eng eng info:eu-repo/semantics/restrictedAccess Goulletquer , P , Wolowicz , M , Latala , A , Brown , C J & Cragg , S 2004 , ' Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas ' , AQUAT LIVING RESOUR , vol. 17 , no. 2 , pp. 195-200 . https://doi.org/10.1051/alr:2004018 MARINE INVERTEBRATE EMBRYOS STARVATION Marine & Freshwater Biology METABOLISM Fisheries OSTREA-EDULIS PHYSIOLOGICAL ENERGETICS OXYGEN-CONSUMPTION GROWTH MODEL SCALLOP TEMPERATURE article 2004 ftuhipublicatio https://doi.org/10.1051/alr:2004018 2024-01-11T23:20:49Z The application of a simple, fast and reliable volumetric microrespirometric method to assess respiration of bivalve larvae is discussed. As a model, C. gigas larvae of various sizes were used. Metabolic activity of veliger larvae was assessed by measuring respiratory rate for use in ecophysiological modelling. As an example of the application of this approach, additional measurements of veliger respiratory rates were carried out to assess the effect on larval metabolism of different concentrations of leachate from wood treated with chromated copper-arsenate (CCA). Veligers of Crassostrea gigas (length from 95 to 331 pm) were fed with a mixture of Isochrysis galbana) and Chaetoceros pumilum cells. Experiments were performed in a 20 degreesC constant seawater temperature. The wet and dry weight of four length (L in pm) classes of larvae were obtained from which the relationship between total dry weight and veliger length was derived {DW = e((3.27+Lx0.0154))} (R-2 = 99%). Moreover, tissue dry weight (TDW; in ng) was calculated according to Gerdes (1983). Since the mathematical model between larval length and respiratory rate explained 88% of the total variability. a more conservative approach using oyster larval dry meat weight (in ng) and respiratory rate (in mu\O-2 h(-1)) was developed to establish a linear model explaining 94.5% of the variability: Resp. = -3.849 x 10(-4) + 5.211 X 10(-6) X TDW. These experiments provided updated figures of C gigas larval respiratory rates for use in ecophysiological models. The relationship between tissue dry weight and respiratory rate was close to previous estimates obtained by Gerdes (1983) and Hoegh-Guldberg and Manahan (1995) at 25 degreesC and 20 degreesC respectively. Our experiments demonstrate that volumetric microrespirometry is suitable for assessing larval respiratory rate and therefore can be used to assess impacts Of Pollutants on an early larval stage. Oysters exposed to leachates from chromated copper arsenate (CCA) treated timber at 5 kg m(-3) showed initially ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster University of the Highlands and Islands: Research Database of UHI Pacific Hoegh ENVELOPE(-62.777,-62.777,-64.830,-64.830) |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
MARINE INVERTEBRATE EMBRYOS STARVATION Marine & Freshwater Biology METABOLISM Fisheries OSTREA-EDULIS PHYSIOLOGICAL ENERGETICS OXYGEN-CONSUMPTION GROWTH MODEL SCALLOP TEMPERATURE |
spellingShingle |
MARINE INVERTEBRATE EMBRYOS STARVATION Marine & Freshwater Biology METABOLISM Fisheries OSTREA-EDULIS PHYSIOLOGICAL ENERGETICS OXYGEN-CONSUMPTION GROWTH MODEL SCALLOP TEMPERATURE Goulletquer, Philippe Wolowicz, M Latala, A Brown, Craig J Cragg, Simon Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas |
topic_facet |
MARINE INVERTEBRATE EMBRYOS STARVATION Marine & Freshwater Biology METABOLISM Fisheries OSTREA-EDULIS PHYSIOLOGICAL ENERGETICS OXYGEN-CONSUMPTION GROWTH MODEL SCALLOP TEMPERATURE |
description |
The application of a simple, fast and reliable volumetric microrespirometric method to assess respiration of bivalve larvae is discussed. As a model, C. gigas larvae of various sizes were used. Metabolic activity of veliger larvae was assessed by measuring respiratory rate for use in ecophysiological modelling. As an example of the application of this approach, additional measurements of veliger respiratory rates were carried out to assess the effect on larval metabolism of different concentrations of leachate from wood treated with chromated copper-arsenate (CCA). Veligers of Crassostrea gigas (length from 95 to 331 pm) were fed with a mixture of Isochrysis galbana) and Chaetoceros pumilum cells. Experiments were performed in a 20 degreesC constant seawater temperature. The wet and dry weight of four length (L in pm) classes of larvae were obtained from which the relationship between total dry weight and veliger length was derived {DW = e((3.27+Lx0.0154))} (R-2 = 99%). Moreover, tissue dry weight (TDW; in ng) was calculated according to Gerdes (1983). Since the mathematical model between larval length and respiratory rate explained 88% of the total variability. a more conservative approach using oyster larval dry meat weight (in ng) and respiratory rate (in mu\O-2 h(-1)) was developed to establish a linear model explaining 94.5% of the variability: Resp. = -3.849 x 10(-4) + 5.211 X 10(-6) X TDW. These experiments provided updated figures of C gigas larval respiratory rates for use in ecophysiological models. The relationship between tissue dry weight and respiratory rate was close to previous estimates obtained by Gerdes (1983) and Hoegh-Guldberg and Manahan (1995) at 25 degreesC and 20 degreesC respectively. Our experiments demonstrate that volumetric microrespirometry is suitable for assessing larval respiratory rate and therefore can be used to assess impacts Of Pollutants on an early larval stage. Oysters exposed to leachates from chromated copper arsenate (CCA) treated timber at 5 kg m(-3) showed initially ... |
format |
Article in Journal/Newspaper |
author |
Goulletquer, Philippe Wolowicz, M Latala, A Brown, Craig J Cragg, Simon |
author_facet |
Goulletquer, Philippe Wolowicz, M Latala, A Brown, Craig J Cragg, Simon |
author_sort |
Goulletquer, Philippe |
title |
Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas |
title_short |
Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas |
title_full |
Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas |
title_fullStr |
Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas |
title_sort |
application of a micro-respirometric volumetric method to respiratory measurements of larvae of the pacific oyster crassostrea gigas |
publishDate |
2004 |
url |
https://pure.uhi.ac.uk/en/publications/f9e411ca-ab23-4135-a53c-d09805199b2c https://doi.org/10.1051/alr:2004018 |
long_lat |
ENVELOPE(-62.777,-62.777,-64.830,-64.830) |
geographic |
Pacific Hoegh |
geographic_facet |
Pacific Hoegh |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Goulletquer , P , Wolowicz , M , Latala , A , Brown , C J & Cragg , S 2004 , ' Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas ' , AQUAT LIVING RESOUR , vol. 17 , no. 2 , pp. 195-200 . https://doi.org/10.1051/alr:2004018 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1051/alr:2004018 |
_version_ |
1789964881208803328 |