One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida)
The DNA barcode within the mitochondrial cox1 gene is typically used to assess the identity and diversity of animals under the assumption that individuals contain a single form of this genetic marker. This study reports on a novel exception from the pelagic tunicate Salpa thompsoni Foxton. Oozoids c...
Published in: | Biological Journal of the Linnean Society |
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ftuhipublicatio:oai:pure.atira.dk:publications/df89580e-e895-4b3e-bf0b-c38a38da00b9 2024-06-23T07:46:09+00:00 One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) Goodall-Copestake, William P. 2016-10-26 application/pdf https://pure.uhi.ac.uk/en/publications/df89580e-e895-4b3e-bf0b-c38a38da00b9 https://doi.org/10.1111/bij.12915 https://pureadmin.uhi.ac.uk/ws/files/14266597/AOV_dynamic_barcodes_in_salps.pdf https://academic.oup.com/biolinnean/article-lookup/doi/10.1111/bij.12915 eng eng https://pure.uhi.ac.uk/en/publications/df89580e-e895-4b3e-bf0b-c38a38da00b9 info:eu-repo/semantics/closedAccess Goodall-Copestake , W P 2016 , ' One tunic but more than one barcode: evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) ' , Biological Journal of the Linnean Society , vol. 120 , no. 3 , pp. 637-648 . https://doi.org/10.1111/bij.12915 Antarctica cox1 cytochrome c oxidase subunit 1 heteroplasmy minicircles recombination salps sublimons tunicate article 2016 ftuhipublicatio https://doi.org/10.1111/bij.12915 2024-06-03T23:48:37Z The DNA barcode within the mitochondrial cox1 gene is typically used to assess the identity and diversity of animals under the assumption that individuals contain a single form of this genetic marker. This study reports on a novel exception from the pelagic tunicate Salpa thompsoni Foxton. Oozoids caught off South Georgia and the Antarctic Peninsula generated barcodes consisting of a single prominent DNA sequence with some additional, subtler signals of intra-individual variation. Further investigation revealed this was due to duplicated and/or minicircular DNAs. These could not simply be explained as artefacts or nuclear copies of mitochondrial DNA, but provided evidence for heteroplasmy arising from a dynamic mitochondrial genome. Genetic variation of this sort may allow S. thompsoni to ecologically benefit from asexually driven population blooms without incurring the genetic cost of an excessive mutational load. Analysis of the prominent barcode sequence data yielded low haplotype (h < 0.61) and nucleotide (π < 0.0014) diversities, and no evidence for genetic structure between sampling locations as assessed using analysis of molecular variance. These results are consistent with the impact of population blooms and the mixing effect of Southern Ocean currents on S. thompsoni genetic diversity. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean University of the Highlands and Islands: Research Database of UHI Antarctic Southern Ocean The Antarctic Antarctic Peninsula Biological Journal of the Linnean Society |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
Antarctica cox1 cytochrome c oxidase subunit 1 heteroplasmy minicircles recombination salps sublimons tunicate |
spellingShingle |
Antarctica cox1 cytochrome c oxidase subunit 1 heteroplasmy minicircles recombination salps sublimons tunicate Goodall-Copestake, William P. One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) |
topic_facet |
Antarctica cox1 cytochrome c oxidase subunit 1 heteroplasmy minicircles recombination salps sublimons tunicate |
description |
The DNA barcode within the mitochondrial cox1 gene is typically used to assess the identity and diversity of animals under the assumption that individuals contain a single form of this genetic marker. This study reports on a novel exception from the pelagic tunicate Salpa thompsoni Foxton. Oozoids caught off South Georgia and the Antarctic Peninsula generated barcodes consisting of a single prominent DNA sequence with some additional, subtler signals of intra-individual variation. Further investigation revealed this was due to duplicated and/or minicircular DNAs. These could not simply be explained as artefacts or nuclear copies of mitochondrial DNA, but provided evidence for heteroplasmy arising from a dynamic mitochondrial genome. Genetic variation of this sort may allow S. thompsoni to ecologically benefit from asexually driven population blooms without incurring the genetic cost of an excessive mutational load. Analysis of the prominent barcode sequence data yielded low haplotype (h < 0.61) and nucleotide (π < 0.0014) diversities, and no evidence for genetic structure between sampling locations as assessed using analysis of molecular variance. These results are consistent with the impact of population blooms and the mixing effect of Southern Ocean currents on S. thompsoni genetic diversity. |
format |
Article in Journal/Newspaper |
author |
Goodall-Copestake, William P. |
author_facet |
Goodall-Copestake, William P. |
author_sort |
Goodall-Copestake, William P. |
title |
One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) |
title_short |
One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) |
title_full |
One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) |
title_fullStr |
One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) |
title_full_unstemmed |
One tunic but more than one barcode::evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) |
title_sort |
one tunic but more than one barcode::evolutionary insights from dynamic mitochondrial dna in salpa thompsoni (tunicata: salpida) |
publishDate |
2016 |
url |
https://pure.uhi.ac.uk/en/publications/df89580e-e895-4b3e-bf0b-c38a38da00b9 https://doi.org/10.1111/bij.12915 https://pureadmin.uhi.ac.uk/ws/files/14266597/AOV_dynamic_barcodes_in_salps.pdf https://academic.oup.com/biolinnean/article-lookup/doi/10.1111/bij.12915 |
geographic |
Antarctic Southern Ocean The Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Southern Ocean The Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean |
op_source |
Goodall-Copestake , W P 2016 , ' One tunic but more than one barcode: evolutionary insights from dynamic mitochondrial DNA in Salpa thompsoni (Tunicata: Salpida) ' , Biological Journal of the Linnean Society , vol. 120 , no. 3 , pp. 637-648 . https://doi.org/10.1111/bij.12915 |
op_relation |
https://pure.uhi.ac.uk/en/publications/df89580e-e895-4b3e-bf0b-c38a38da00b9 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/bij.12915 |
container_title |
Biological Journal of the Linnean Society |
_version_ |
1802644221902979072 |