The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus
Lumpfish (C. lumpus) are used as cleaner fish in the Atlantic salmon (Salmo salar) farming industry to remove parasitic sea lice. At present, wild lumpfish broodstock are used which puts strain on wild populations. By successfully cryopreserving lumpfish sperm, the number of wild males required will...
Published in: | Aquaculture |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2022
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Online Access: | https://pure.uhi.ac.uk/en/publications/c1ed55ac-3e47-4279-af4c-06fd402616f6 https://doi.org/10.1016/j.aquaculture.2021.737466 https://pureadmin.uhi.ac.uk/ws/files/16786081/AQUACULTURE_D_21_01686_R3.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848621011297 |
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ftuhipublicatio:oai:pure.atira.dk:publications/c1ed55ac-3e47-4279-af4c-06fd402616f6 2024-01-28T10:04:35+01:00 The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus Imsland, Albert Kjartan Dagbjartarson Purvis, Emily Reinardy, Helena C. Kapari, Lauri Watts, Ellie Jane Hangstad, Thor Arne 2022-01-01 application/pdf https://pure.uhi.ac.uk/en/publications/c1ed55ac-3e47-4279-af4c-06fd402616f6 https://doi.org/10.1016/j.aquaculture.2021.737466 https://pureadmin.uhi.ac.uk/ws/files/16786081/AQUACULTURE_D_21_01686_R3.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848621011297 eng eng info:eu-repo/semantics/openAccess Imsland , A K D , Purvis , E , Reinardy , H C , Kapari , L , Watts , E J & Hangstad , T A 2022 , ' The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus ' , Aquaculture , vol. 547 , 737466 . https://doi.org/10.1016/j.aquaculture.2021.737466 Lumpfish Cryopreservation of sperm Fertilization hatching sucess article 2022 ftuhipublicatio https://doi.org/10.1016/j.aquaculture.2021.737466 2024-01-04T23:21:28Z Lumpfish (C. lumpus) are used as cleaner fish in the Atlantic salmon (Salmo salar) farming industry to remove parasitic sea lice. At present, wild lumpfish broodstock are used which puts strain on wild populations. By successfully cryopreserving lumpfish sperm, the number of wild males required will be reduced and it enables the long-term storage of sperm for use in breeding programmes. The present study compared the use of fresh sperm and sperm which was cryogenically frozen for 24 h to test whether it is a viable method of preservation. The fresh and frozen sperm from 5 males was used (in equal volumes) to fertilize eggs pooled from 5 females and the difference between fertilization success, percentage of eggs which reached the eyed stage, and the hatching success was measured. A group of 100 hatched larvae were on-grown for two weeks to test whether there was a difference weight between treatment groups. The results of the trial showed that fresh sperm produced a significantly higher percentage of fertilized eggs (fresh 92.6 ± 0.8%, frozen 77.9 ± 1.8%, mean ± SEM), a higher percentage of eggs surviving to the eyed stage (fresh 93.9 ± 0.5% and frozen 80.8 ± 1.4%) and had a more successful hatch rate (fresh 72.3 ± 6.6% and frozen 63.6 ± 5.0%). There was no difference in mean weight (± SEM) of the two-week old larvae between treatments (fresh 0.63 g ± 0.024, frozen 0.59 g ± 0.028). In conclusion, this study showed that the same volume of cryogenically preserved lumpfish sperm produced fewer viable lumpfish larvae than fresh sperm. Despite this difference, the use of cryopreserved sperm did produce relatively high results at each stage of testing. Article in Journal/Newspaper Atlantic salmon Salmo salar University of the Highlands and Islands: Research Database of UHI Aquaculture 547 737466 |
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Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
Lumpfish Cryopreservation of sperm Fertilization hatching sucess |
spellingShingle |
Lumpfish Cryopreservation of sperm Fertilization hatching sucess Imsland, Albert Kjartan Dagbjartarson Purvis, Emily Reinardy, Helena C. Kapari, Lauri Watts, Ellie Jane Hangstad, Thor Arne The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus |
topic_facet |
Lumpfish Cryopreservation of sperm Fertilization hatching sucess |
description |
Lumpfish (C. lumpus) are used as cleaner fish in the Atlantic salmon (Salmo salar) farming industry to remove parasitic sea lice. At present, wild lumpfish broodstock are used which puts strain on wild populations. By successfully cryopreserving lumpfish sperm, the number of wild males required will be reduced and it enables the long-term storage of sperm for use in breeding programmes. The present study compared the use of fresh sperm and sperm which was cryogenically frozen for 24 h to test whether it is a viable method of preservation. The fresh and frozen sperm from 5 males was used (in equal volumes) to fertilize eggs pooled from 5 females and the difference between fertilization success, percentage of eggs which reached the eyed stage, and the hatching success was measured. A group of 100 hatched larvae were on-grown for two weeks to test whether there was a difference weight between treatment groups. The results of the trial showed that fresh sperm produced a significantly higher percentage of fertilized eggs (fresh 92.6 ± 0.8%, frozen 77.9 ± 1.8%, mean ± SEM), a higher percentage of eggs surviving to the eyed stage (fresh 93.9 ± 0.5% and frozen 80.8 ± 1.4%) and had a more successful hatch rate (fresh 72.3 ± 6.6% and frozen 63.6 ± 5.0%). There was no difference in mean weight (± SEM) of the two-week old larvae between treatments (fresh 0.63 g ± 0.024, frozen 0.59 g ± 0.028). In conclusion, this study showed that the same volume of cryogenically preserved lumpfish sperm produced fewer viable lumpfish larvae than fresh sperm. Despite this difference, the use of cryopreserved sperm did produce relatively high results at each stage of testing. |
format |
Article in Journal/Newspaper |
author |
Imsland, Albert Kjartan Dagbjartarson Purvis, Emily Reinardy, Helena C. Kapari, Lauri Watts, Ellie Jane Hangstad, Thor Arne |
author_facet |
Imsland, Albert Kjartan Dagbjartarson Purvis, Emily Reinardy, Helena C. Kapari, Lauri Watts, Ellie Jane Hangstad, Thor Arne |
author_sort |
Imsland, Albert Kjartan Dagbjartarson |
title |
The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus |
title_short |
The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus |
title_full |
The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus |
title_fullStr |
The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus |
title_full_unstemmed |
The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus |
title_sort |
effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish c. lumpus |
publishDate |
2022 |
url |
https://pure.uhi.ac.uk/en/publications/c1ed55ac-3e47-4279-af4c-06fd402616f6 https://doi.org/10.1016/j.aquaculture.2021.737466 https://pureadmin.uhi.ac.uk/ws/files/16786081/AQUACULTURE_D_21_01686_R3.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848621011297 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Imsland , A K D , Purvis , E , Reinardy , H C , Kapari , L , Watts , E J & Hangstad , T A 2022 , ' The effects of cryogenically preserved sperm on the fertilization, embryonic development and hatching success of lumpfish C. lumpus ' , Aquaculture , vol. 547 , 737466 . https://doi.org/10.1016/j.aquaculture.2021.737466 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2021.737466 |
container_title |
Aquaculture |
container_volume |
547 |
container_start_page |
737466 |
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1789330337512292352 |