Effect of elevated CO2 on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord

• Published in: Biogeosciences
• Main Authors: Sperling, M., Piontek, J., Gerdts, G., Wichels, A., Schunck, H., Roy, A. S., La Roche, J., Gilbert, J., Nissimov, J. I., Bittner, L., Romac, S., Riebesell, U., Engel, A.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2013
• Subjects: Arctic; Svalbard; Kongsfjord*; Kongsfjorden; Ocean acidification; Phytoplankton
• Online Access: https://pure.uhi.ac.uk/en/publications/bb788853-e863-4fc5-b27b-268c8d65d9b3; https://doi.org/10.5194/bg-10-181-2013; http://www.scopus.com/inward/record.url?scp=84872229165&partnerID=8YFLogxK; http://www.scopus.com/inward/citedby.url?scp=84872229165&partnerID=8YFLogxK

In the frame of the European Project on Ocean Acidification (EPOCA), the response of an Arctic pelagic community (<3 mm) to a gradient of seawater pCO 2 was investigated. For this purpose 9 large-scale in situ mesocosms were deployed in Kongsfjorden, Svalbard (78° 56.2' N, 11° 53.6' E), in 2010. The present study investigates effects on the communities of particle-attached (PA; >3 μm) and free-living (FL; < 3 μm > 0.2 μm) bacteria by Automated Ribosomal Intergenic Spacer Analysis (ARISA) in 6 of the mesocosms, ranging from 185 to 1050 μatm initial pCO 2 , and the surrounding fjord. ARISA was able to resolve, on average, 27 bacterial band classes per sample and allowed for a detailed investigation of the explicit richness and diversity. Both, the PA and the FL bacterioplankton community exhibited a strong temporal development, which was driven mainly by temperature and phytoplankton development. In response to the breakdown of a picophytoplankton bloom, numbers of ARISA band classes in the PA community were reduced at low and medium CO 2 (̃ 185-685 μatm) by about 25%, while they were more or less stable at high CO 2 (̃ 820-1050 μatm). We hypothesise that enhanced viral lysis and enhanced availability of organic substrates at high CO 2 resulted in a more diverse PA bacterial community in the post-bloom phase. Despite lower cell numbers and extracellular enzyme activities in the post-bloom phase, bacterial protein production was enhanced in high CO 2 mesocosms, suggesting a positive effect of community richness on this function and on carbon cycling by bacteria.