nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics
Identifying genetic cues of functional relevance is key to understanding the drivers of evolution and increasingly important for the conservation of biodiversity. This study introduces nuclear ribosomal DNA (nrDNA) to mitochondrial DNA (mtDNA) copy number ratios as a metric with which to screen for...
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Online Access: | https://pure.uhi.ac.uk/en/publications/b646178f-1e16-43f8-bd9e-937034ad0787 https://doi.org/10.1038/s41437-018-0088-8 https://pureadmin.uhi.ac.uk/ws/files/13901216/heredity_v121_p105_111_2018_accepted_ms.pdf http://www.nature.com/articles/s41437-018-0088-8 |
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ftuhipublicatio:oai:pure.atira.dk:publications/b646178f-1e16-43f8-bd9e-937034ad0787 2024-06-23T07:52:28+00:00 nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics Goodall-Copestake, William Paul 2018-08-01 application/pdf https://pure.uhi.ac.uk/en/publications/b646178f-1e16-43f8-bd9e-937034ad0787 https://doi.org/10.1038/s41437-018-0088-8 https://pureadmin.uhi.ac.uk/ws/files/13901216/heredity_v121_p105_111_2018_accepted_ms.pdf http://www.nature.com/articles/s41437-018-0088-8 eng eng https://pure.uhi.ac.uk/en/publications/b646178f-1e16-43f8-bd9e-937034ad0787 info:eu-repo/semantics/openAccess Goodall-Copestake , W P 2018 , ' nrDNA: mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics ' , Heredity , vol. 121 , no. 2 , pp. 105-111 . https://doi.org/10.1038/s41437-018-0088-8 Ecological genetics Evolutionary genetics Genetic markers article 2018 ftuhipublicatio https://doi.org/10.1038/s41437-018-0088-8 2024-06-03T23:48:37Z Identifying genetic cues of functional relevance is key to understanding the drivers of evolution and increasingly important for the conservation of biodiversity. This study introduces nuclear ribosomal DNA (nrDNA) to mitochondrial DNA (mtDNA) copy number ratios as a metric with which to screen for this functional genetic variation prior to more extensive omics analyses. To illustrate the metric, quantitative PCR was used to estimate nrDNA (18S) to mtDNA (16S) copy number ratios in muscle tissue from samples of two zooplankton species: Salpa thompsoni caught near Elephant Island (Southern Ocean) and S. fusiformis sampled off Gough Island (South Atlantic). Average 18S:16S ratios in these samples were 9:1 and 3:1, respectively. nrDNA 45S arrays and mitochondrial genomes were then deep sequenced to uncover the sources of intra-individual genetic variation underlying these 18S:16S copy number differences. The deep sequencing profiles obtained were consistent with genetic changes resulting from adaptive processes, including an expansion of nrDNA and damage to mtDNA in S. thompsoni, potentially in response to the polar environment. Beyond this example from zooplankton, nrDNA:mtDNA copy number ratios offer a promising metric to help identify genetic variation of functional relevance in animals more broadly. Article in Journal/Newspaper Elephant Island Southern Ocean University of the Highlands and Islands: Research Database of UHI Southern Ocean Elephant Island ENVELOPE(-55.184,-55.184,-61.085,-61.085) Gough ENVELOPE(159.367,159.367,-81.633,-81.633) Heredity 121 2 105 111 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
Ecological genetics Evolutionary genetics Genetic markers |
spellingShingle |
Ecological genetics Evolutionary genetics Genetic markers Goodall-Copestake, William Paul nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics |
topic_facet |
Ecological genetics Evolutionary genetics Genetic markers |
description |
Identifying genetic cues of functional relevance is key to understanding the drivers of evolution and increasingly important for the conservation of biodiversity. This study introduces nuclear ribosomal DNA (nrDNA) to mitochondrial DNA (mtDNA) copy number ratios as a metric with which to screen for this functional genetic variation prior to more extensive omics analyses. To illustrate the metric, quantitative PCR was used to estimate nrDNA (18S) to mtDNA (16S) copy number ratios in muscle tissue from samples of two zooplankton species: Salpa thompsoni caught near Elephant Island (Southern Ocean) and S. fusiformis sampled off Gough Island (South Atlantic). Average 18S:16S ratios in these samples were 9:1 and 3:1, respectively. nrDNA 45S arrays and mitochondrial genomes were then deep sequenced to uncover the sources of intra-individual genetic variation underlying these 18S:16S copy number differences. The deep sequencing profiles obtained were consistent with genetic changes resulting from adaptive processes, including an expansion of nrDNA and damage to mtDNA in S. thompsoni, potentially in response to the polar environment. Beyond this example from zooplankton, nrDNA:mtDNA copy number ratios offer a promising metric to help identify genetic variation of functional relevance in animals more broadly. |
format |
Article in Journal/Newspaper |
author |
Goodall-Copestake, William Paul |
author_facet |
Goodall-Copestake, William Paul |
author_sort |
Goodall-Copestake, William Paul |
title |
nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics |
title_short |
nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics |
title_full |
nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics |
title_fullStr |
nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics |
title_full_unstemmed |
nrDNA::mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics |
title_sort |
nrdna::mtdna copy number ratios as a comparative metric for evolutionary and conservation genetics |
publishDate |
2018 |
url |
https://pure.uhi.ac.uk/en/publications/b646178f-1e16-43f8-bd9e-937034ad0787 https://doi.org/10.1038/s41437-018-0088-8 https://pureadmin.uhi.ac.uk/ws/files/13901216/heredity_v121_p105_111_2018_accepted_ms.pdf http://www.nature.com/articles/s41437-018-0088-8 |
long_lat |
ENVELOPE(-55.184,-55.184,-61.085,-61.085) ENVELOPE(159.367,159.367,-81.633,-81.633) |
geographic |
Southern Ocean Elephant Island Gough |
geographic_facet |
Southern Ocean Elephant Island Gough |
genre |
Elephant Island Southern Ocean |
genre_facet |
Elephant Island Southern Ocean |
op_source |
Goodall-Copestake , W P 2018 , ' nrDNA: mtDNA copy number ratios as a comparative metric for evolutionary and conservation genetics ' , Heredity , vol. 121 , no. 2 , pp. 105-111 . https://doi.org/10.1038/s41437-018-0088-8 |
op_relation |
https://pure.uhi.ac.uk/en/publications/b646178f-1e16-43f8-bd9e-937034ad0787 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41437-018-0088-8 |
container_title |
Heredity |
container_volume |
121 |
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2 |
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105 |
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111 |
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1802643769563021312 |