Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments
Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidi...
Published in: | Frontiers in Microbiology |
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2017
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Online Access: | https://pure.uhi.ac.uk/en/publications/8fbf588c-1079-4b13-9277-78ccfc6ccb63 https://doi.org/10.3389/fmicb.2017.01599 https://pureadmin.uhi.ac.uk/ws/files/2393585/fmicb_08_01599.pdf http://journal.frontiersin.org/article/10.3389/fmicb.2017.01599/full#supplementary-material |
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ftuhipublicatio:oai:pure.atira.dk:publications/8fbf588c-1079-4b13-9277-78ccfc6ccb63 2024-09-15T18:28:19+00:00 Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments Currie, Ashleigh Tait, Karen Parry, Helen de Francisco Mora, Beatriz Hicks, Natalie Osborn, Andrew M Widdicombe, Steve Stahl, Henrik 2017-08-22 application/pdf https://pure.uhi.ac.uk/en/publications/8fbf588c-1079-4b13-9277-78ccfc6ccb63 https://doi.org/10.3389/fmicb.2017.01599 https://pureadmin.uhi.ac.uk/ws/files/2393585/fmicb_08_01599.pdf http://journal.frontiersin.org/article/10.3389/fmicb.2017.01599/full#supplementary-material eng eng https://pure.uhi.ac.uk/en/publications/8fbf588c-1079-4b13-9277-78ccfc6ccb63 info:eu-repo/semantics/openAccess Currie , A , Tait , K , Parry , H , de Francisco Mora , B , Hicks , N , Osborn , A M , Widdicombe , S & Stahl , H 2017 , ' Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments ' , Frontiers in Microbiology . https://doi.org/10.3389/fmicb.2017.01599 article 2017 ftuhipublicatio https://doi.org/10.3389/fmicb.2017.01599 2024-07-22T23:37:24Z Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient + 4 °C) on the abundance of taxonomic and functional microbial genes. Specific q-PCR primers were used to target archaeal, bacterial and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and potential impacts on nitrogen cycling. In muddy sediment, microbial gene abundance, including amoA and nirS genes, increased under elevated temperature and reduced under elevated CO2 after 28 days, accompanied by shifts in community composition. In contrast, the combined stressor treatment showed a non-additive effect with lower microbial gene abundance throughout the experiment. The response of microbial communities in the sandy sediment was less pronounced, with the most noticeable response seen in the archaeal gene abundances in response to environmental stressors over time. 16S rRNA genes (amoA and nirS) were lower in abundance in the combined stressor treatments in sandy sediments. Our results indicated that marine benthic microorganisms, especially in muddy sediments, are susceptible to changes in ocean carbonate chemistry and seawater temperature, which ultimately may have an impact upon key benthic biogeochemical cycles. Article in Journal/Newspaper Ocean acidification University of the Highlands and Islands: Research Database of UHI Frontiers in Microbiology 8 |
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University of the Highlands and Islands: Research Database of UHI |
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ftuhipublicatio |
language |
English |
description |
Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient + 4 °C) on the abundance of taxonomic and functional microbial genes. Specific q-PCR primers were used to target archaeal, bacterial and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and potential impacts on nitrogen cycling. In muddy sediment, microbial gene abundance, including amoA and nirS genes, increased under elevated temperature and reduced under elevated CO2 after 28 days, accompanied by shifts in community composition. In contrast, the combined stressor treatment showed a non-additive effect with lower microbial gene abundance throughout the experiment. The response of microbial communities in the sandy sediment was less pronounced, with the most noticeable response seen in the archaeal gene abundances in response to environmental stressors over time. 16S rRNA genes (amoA and nirS) were lower in abundance in the combined stressor treatments in sandy sediments. Our results indicated that marine benthic microorganisms, especially in muddy sediments, are susceptible to changes in ocean carbonate chemistry and seawater temperature, which ultimately may have an impact upon key benthic biogeochemical cycles. |
format |
Article in Journal/Newspaper |
author |
Currie, Ashleigh Tait, Karen Parry, Helen de Francisco Mora, Beatriz Hicks, Natalie Osborn, Andrew M Widdicombe, Steve Stahl, Henrik |
spellingShingle |
Currie, Ashleigh Tait, Karen Parry, Helen de Francisco Mora, Beatriz Hicks, Natalie Osborn, Andrew M Widdicombe, Steve Stahl, Henrik Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments |
author_facet |
Currie, Ashleigh Tait, Karen Parry, Helen de Francisco Mora, Beatriz Hicks, Natalie Osborn, Andrew M Widdicombe, Steve Stahl, Henrik |
author_sort |
Currie, Ashleigh |
title |
Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments |
title_short |
Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments |
title_full |
Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments |
title_fullStr |
Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments |
title_full_unstemmed |
Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments |
title_sort |
marine microbial gene abundance and community composition in response to ocean acidification and elevated temperature in two contrasting coastal marine sediments |
publishDate |
2017 |
url |
https://pure.uhi.ac.uk/en/publications/8fbf588c-1079-4b13-9277-78ccfc6ccb63 https://doi.org/10.3389/fmicb.2017.01599 https://pureadmin.uhi.ac.uk/ws/files/2393585/fmicb_08_01599.pdf http://journal.frontiersin.org/article/10.3389/fmicb.2017.01599/full#supplementary-material |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Currie , A , Tait , K , Parry , H , de Francisco Mora , B , Hicks , N , Osborn , A M , Widdicombe , S & Stahl , H 2017 , ' Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments ' , Frontiers in Microbiology . https://doi.org/10.3389/fmicb.2017.01599 |
op_relation |
https://pure.uhi.ac.uk/en/publications/8fbf588c-1079-4b13-9277-78ccfc6ccb63 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2017.01599 |
container_title |
Frontiers in Microbiology |
container_volume |
8 |
_version_ |
1810469661940121600 |