Stability of the lipid biomarker H-Print within preserved animals
Highly branched isoprenoid lipid (HBI) biomarkers have been reported in a wide range of Arctic animals spanning all trophic levels. The HBI biomarker-based ‘H-Print’ has been used to estimate the relative contributions of sea ice- and phytoplankton-derived primary production utilised by Arctic anima...
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ftuhipublicatio:oai:pure.atira.dk:publications/850f26d3-d64b-4917-a190-1b4a5af3e32b 2024-01-28T10:03:19+01:00 Stability of the lipid biomarker H-Print within preserved animals Brown, Thomas Adam 2018-03-23 application/vnd.openxmlformats-officedocument.wordprocessingml.document https://pure.uhi.ac.uk/en/publications/850f26d3-d64b-4917-a190-1b4a5af3e32b https://doi.org/10.1007/s00300-018-2317-2 https://pureadmin.uhi.ac.uk/ws/files/3206177/Brown_2018_PURE.docx eng eng info:eu-repo/semantics/openAccess Brown , T A 2018 , ' Stability of the lipid biomarker H-Print within preserved animals ' , Polar Biology , pp. 1 . https://doi.org/10.1007/s00300-018-2317-2 H-print HBI IP25 Storage Food web Sea ice primary production article 2018 ftuhipublicatio https://doi.org/10.1007/s00300-018-2317-2 2024-01-04T23:21:06Z Highly branched isoprenoid lipid (HBI) biomarkers have been reported in a wide range of Arctic animals spanning all trophic levels. The HBI biomarker-based ‘H-Print’ has been used to estimate the relative contributions of sea ice- and phytoplankton-derived primary production utilised by Arctic animals. The resulting data are providing insight into the future impact of climate change-associated sea ice decline on ecosystems. Given the range of methods available to preserve biological tissues between sampling and analysis, it was considered important to test the suitability of these methods for storage prior to the analysis of HBI lipids. In the laboratory, Artemia sp. were fed diatoms containing HBIs of known proportion. These were then harvested and stored wet- and dry-frozen and in ethanol and 4% formalin. After 6 months, Artemia sp. H-Prints stored in ethanol were significantly different from the control, whereas H-Prints from all other methods were not significantly different from the control. Dry-frozen Artemia sp. H-Prints bared the closest similarity to the control, representing the best method tested for tissue preservation prior to analysis of HBIs. This study will contribute to improving the robustness of HBI-based H-Print analyses in rapidly changing Arctic ecosystems. Article in Journal/Newspaper Arctic Climate change Phytoplankton Polar Biology Sea ice University of the Highlands and Islands: Research Database of UHI Arctic Polar Biology 41 9 1901 1905 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
H-print HBI IP25 Storage Food web Sea ice primary production |
spellingShingle |
H-print HBI IP25 Storage Food web Sea ice primary production Brown, Thomas Adam Stability of the lipid biomarker H-Print within preserved animals |
topic_facet |
H-print HBI IP25 Storage Food web Sea ice primary production |
description |
Highly branched isoprenoid lipid (HBI) biomarkers have been reported in a wide range of Arctic animals spanning all trophic levels. The HBI biomarker-based ‘H-Print’ has been used to estimate the relative contributions of sea ice- and phytoplankton-derived primary production utilised by Arctic animals. The resulting data are providing insight into the future impact of climate change-associated sea ice decline on ecosystems. Given the range of methods available to preserve biological tissues between sampling and analysis, it was considered important to test the suitability of these methods for storage prior to the analysis of HBI lipids. In the laboratory, Artemia sp. were fed diatoms containing HBIs of known proportion. These were then harvested and stored wet- and dry-frozen and in ethanol and 4% formalin. After 6 months, Artemia sp. H-Prints stored in ethanol were significantly different from the control, whereas H-Prints from all other methods were not significantly different from the control. Dry-frozen Artemia sp. H-Prints bared the closest similarity to the control, representing the best method tested for tissue preservation prior to analysis of HBIs. This study will contribute to improving the robustness of HBI-based H-Print analyses in rapidly changing Arctic ecosystems. |
format |
Article in Journal/Newspaper |
author |
Brown, Thomas Adam |
author_facet |
Brown, Thomas Adam |
author_sort |
Brown, Thomas Adam |
title |
Stability of the lipid biomarker H-Print within preserved animals |
title_short |
Stability of the lipid biomarker H-Print within preserved animals |
title_full |
Stability of the lipid biomarker H-Print within preserved animals |
title_fullStr |
Stability of the lipid biomarker H-Print within preserved animals |
title_full_unstemmed |
Stability of the lipid biomarker H-Print within preserved animals |
title_sort |
stability of the lipid biomarker h-print within preserved animals |
publishDate |
2018 |
url |
https://pure.uhi.ac.uk/en/publications/850f26d3-d64b-4917-a190-1b4a5af3e32b https://doi.org/10.1007/s00300-018-2317-2 https://pureadmin.uhi.ac.uk/ws/files/3206177/Brown_2018_PURE.docx |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change Phytoplankton Polar Biology Sea ice |
genre_facet |
Arctic Climate change Phytoplankton Polar Biology Sea ice |
op_source |
Brown , T A 2018 , ' Stability of the lipid biomarker H-Print within preserved animals ' , Polar Biology , pp. 1 . https://doi.org/10.1007/s00300-018-2317-2 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00300-018-2317-2 |
container_title |
Polar Biology |
container_volume |
41 |
container_issue |
9 |
container_start_page |
1901 |
op_container_end_page |
1905 |
_version_ |
1789328712095760384 |