Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland)
We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail befor...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | https://pure.uhi.ac.uk/en/publications/79bd45c8-e549-450d-b15c-a072059bfc30 https://doi.org/10.1111/j.1462-2920.2006.01103.x |
id |
ftuhipublicatio:oai:pure.atira.dk:publications/79bd45c8-e549-450d-b15c-a072059bfc30 |
---|---|
record_format |
openpolar |
spelling |
ftuhipublicatio:oai:pure.atira.dk:publications/79bd45c8-e549-450d-b15c-a072059bfc30 2024-06-23T07:47:48+00:00 Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) Roeselers, G Norris, TB Castenholz, RW Rysgaard, S Glud, Ronnie Kuhl, M Muyzer, G 2007 https://pure.uhi.ac.uk/en/publications/79bd45c8-e549-450d-b15c-a072059bfc30 https://doi.org/10.1111/j.1462-2920.2006.01103.x eng eng https://pure.uhi.ac.uk/en/publications/79bd45c8-e549-450d-b15c-a072059bfc30 info:eu-repo/semantics/restrictedAccess Roeselers , G , Norris , TB , Castenholz , RW , Rysgaard , S , Glud , R , Kuhl , M & Muyzer , G 2007 , ' Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) ' , ENVIRON MICROBIOL , vol. 9 , no. 1 , pp. 26-38 . https://doi.org/10.1111/j.1462-2920.2006.01103.x COMMUNITIES CYANOBACTERIA POPULATIONS MOLECULAR APPROACH Microbiology SULFIDE RADIATION TEMPERATURE CHLOROFLEXUS ANTARCTIC LAKE ICE GRADIENT GEL-ELECTROPHORESIS article 2007 ftuhipublicatio https://doi.org/10.1111/j.1462-2920.2006.01103.x 2024-06-10T23:47:56Z We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime. Article in Journal/Newspaper Antarc* Antarctic Arctic Arctic Greenland University of the Highlands and Islands: Research Database of UHI Antarctic Arctic Greenland Environmental Microbiology 9 1 26 38 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
COMMUNITIES CYANOBACTERIA POPULATIONS MOLECULAR APPROACH Microbiology SULFIDE RADIATION TEMPERATURE CHLOROFLEXUS ANTARCTIC LAKE ICE GRADIENT GEL-ELECTROPHORESIS |
spellingShingle |
COMMUNITIES CYANOBACTERIA POPULATIONS MOLECULAR APPROACH Microbiology SULFIDE RADIATION TEMPERATURE CHLOROFLEXUS ANTARCTIC LAKE ICE GRADIENT GEL-ELECTROPHORESIS Roeselers, G Norris, TB Castenholz, RW Rysgaard, S Glud, Ronnie Kuhl, M Muyzer, G Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) |
topic_facet |
COMMUNITIES CYANOBACTERIA POPULATIONS MOLECULAR APPROACH Microbiology SULFIDE RADIATION TEMPERATURE CHLOROFLEXUS ANTARCTIC LAKE ICE GRADIENT GEL-ELECTROPHORESIS |
description |
We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime. |
format |
Article in Journal/Newspaper |
author |
Roeselers, G Norris, TB Castenholz, RW Rysgaard, S Glud, Ronnie Kuhl, M Muyzer, G |
author_facet |
Roeselers, G Norris, TB Castenholz, RW Rysgaard, S Glud, Ronnie Kuhl, M Muyzer, G |
author_sort |
Roeselers, G |
title |
Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) |
title_short |
Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) |
title_full |
Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) |
title_fullStr |
Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) |
title_full_unstemmed |
Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) |
title_sort |
diversity of phototrophic bacteria in microbial mats from arctic hot springs (greenland) |
publishDate |
2007 |
url |
https://pure.uhi.ac.uk/en/publications/79bd45c8-e549-450d-b15c-a072059bfc30 https://doi.org/10.1111/j.1462-2920.2006.01103.x |
geographic |
Antarctic Arctic Greenland |
geographic_facet |
Antarctic Arctic Greenland |
genre |
Antarc* Antarctic Arctic Arctic Greenland |
genre_facet |
Antarc* Antarctic Arctic Arctic Greenland |
op_source |
Roeselers , G , Norris , TB , Castenholz , RW , Rysgaard , S , Glud , R , Kuhl , M & Muyzer , G 2007 , ' Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland) ' , ENVIRON MICROBIOL , vol. 9 , no. 1 , pp. 26-38 . https://doi.org/10.1111/j.1462-2920.2006.01103.x |
op_relation |
https://pure.uhi.ac.uk/en/publications/79bd45c8-e549-450d-b15c-a072059bfc30 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1462-2920.2006.01103.x |
container_title |
Environmental Microbiology |
container_volume |
9 |
container_issue |
1 |
container_start_page |
26 |
op_container_end_page |
38 |
_version_ |
1802637978766409728 |