Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns
Discrimination of species of Atlantic sandeels (genus Ammodytes) using morphological characters can be problematic, particularly for juveniles and small adults. To identify genetic markers, four species of sandeel, Ammodytes marinus Raitt, A. tobianus L., A. dubius Reinhardt and Gymnammodytes semisq...
Published in: | Fisheries Research |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
1998
|
Subjects: | |
Online Access: | https://pure.uhi.ac.uk/en/publications/762eeb45-7cd8-4535-afbb-f133689fccb9 https://doi.org/10.1016/S0165-7836(98)00081-2 |
id |
ftuhipublicatio:oai:pure.atira.dk:publications/762eeb45-7cd8-4535-afbb-f133689fccb9 |
---|---|
record_format |
openpolar |
spelling |
ftuhipublicatio:oai:pure.atira.dk:publications/762eeb45-7cd8-4535-afbb-f133689fccb9 2024-06-23T07:55:00+00:00 Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns Mitchell, A McCarthy, E Verspoor, E 1998-06-01 https://pure.uhi.ac.uk/en/publications/762eeb45-7cd8-4535-afbb-f133689fccb9 https://doi.org/10.1016/S0165-7836(98)00081-2 eng eng https://pure.uhi.ac.uk/en/publications/762eeb45-7cd8-4535-afbb-f133689fccb9 info:eu-repo/semantics/restrictedAccess Mitchell , A , McCarthy , E & Verspoor , E 1998 , ' Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns ' , Fisheries Research , vol. 36 , no. 1 , pp. 61-65 . https://doi.org/10.1016/S0165-7836(98)00081-2 sandeels Ammodytes spp Gymnammodytes semisquamatus mitochondrial DNA markers article 1998 ftuhipublicatio https://doi.org/10.1016/S0165-7836(98)00081-2 2024-05-27T23:51:24Z Discrimination of species of Atlantic sandeels (genus Ammodytes) using morphological characters can be problematic, particularly for juveniles and small adults. To identify genetic markers, four species of sandeel, Ammodytes marinus Raitt, A. tobianus L., A. dubius Reinhardt and Gymnammodytes semisquamatus Jourdain, were screened for diagnostic variation in the PCR-amplified ND-1 region of the mitochondrial genome. Seven restriction endonucleases were used: AluI, AvaI, CfoI, DraI, HaeIII, HinfI and MvaI. Alu, and HaeIII produced a unique range of fragment patterns in each species. In addition, CfoI distinguished the three Northeast Atlantic species, while AvaI distinguished Ammodytes from Gymnammodytes. These mitochondrial species markers have advantages over previously described protein markers as, in addition to being fast and technically simple, results can be obtained from small amounts of frozen or alcohol-preserved tissue. Thus, the described markers provide useful discriminators for fisheries assessments and ecological studies. Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A. tobianus, A. dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns. Available from: http://www.researchgate.net/publication/248423806_Discrimination_of_the_North_Atlantic_lesser_sandeels_Ammodytes_marinus_A._tobianus_A._dubius_and_Gymnammodytes_semisquamatus_by_mitochondrial_DNA_restriction_fragment_patterns [accessed Jun 4, 2015]. Article in Journal/Newspaper North Atlantic Northeast Atlantic University of the Highlands and Islands: Research Database of UHI Reinhardt ENVELOPE(177.200,177.200,-84.200,-84.200) Fisheries Research 36 1 61 65 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
sandeels Ammodytes spp Gymnammodytes semisquamatus mitochondrial DNA markers |
spellingShingle |
sandeels Ammodytes spp Gymnammodytes semisquamatus mitochondrial DNA markers Mitchell, A McCarthy, E Verspoor, E Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns |
topic_facet |
sandeels Ammodytes spp Gymnammodytes semisquamatus mitochondrial DNA markers |
description |
Discrimination of species of Atlantic sandeels (genus Ammodytes) using morphological characters can be problematic, particularly for juveniles and small adults. To identify genetic markers, four species of sandeel, Ammodytes marinus Raitt, A. tobianus L., A. dubius Reinhardt and Gymnammodytes semisquamatus Jourdain, were screened for diagnostic variation in the PCR-amplified ND-1 region of the mitochondrial genome. Seven restriction endonucleases were used: AluI, AvaI, CfoI, DraI, HaeIII, HinfI and MvaI. Alu, and HaeIII produced a unique range of fragment patterns in each species. In addition, CfoI distinguished the three Northeast Atlantic species, while AvaI distinguished Ammodytes from Gymnammodytes. These mitochondrial species markers have advantages over previously described protein markers as, in addition to being fast and technically simple, results can be obtained from small amounts of frozen or alcohol-preserved tissue. Thus, the described markers provide useful discriminators for fisheries assessments and ecological studies. Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A. tobianus, A. dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns. Available from: http://www.researchgate.net/publication/248423806_Discrimination_of_the_North_Atlantic_lesser_sandeels_Ammodytes_marinus_A._tobianus_A._dubius_and_Gymnammodytes_semisquamatus_by_mitochondrial_DNA_restriction_fragment_patterns [accessed Jun 4, 2015]. |
format |
Article in Journal/Newspaper |
author |
Mitchell, A McCarthy, E Verspoor, E |
author_facet |
Mitchell, A McCarthy, E Verspoor, E |
author_sort |
Mitchell, A |
title |
Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns |
title_short |
Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns |
title_full |
Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns |
title_fullStr |
Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns |
title_full_unstemmed |
Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns |
title_sort |
discrimination of the north atlantic lesser sandeels ammodytes marinus, a-tobianus, a-dubius and gymnammodytes semisquamatus by mitochondrial dna restriction fragment patterns |
publishDate |
1998 |
url |
https://pure.uhi.ac.uk/en/publications/762eeb45-7cd8-4535-afbb-f133689fccb9 https://doi.org/10.1016/S0165-7836(98)00081-2 |
long_lat |
ENVELOPE(177.200,177.200,-84.200,-84.200) |
geographic |
Reinhardt |
geographic_facet |
Reinhardt |
genre |
North Atlantic Northeast Atlantic |
genre_facet |
North Atlantic Northeast Atlantic |
op_source |
Mitchell , A , McCarthy , E & Verspoor , E 1998 , ' Discrimination of the North Atlantic lesser sandeels Ammodytes marinus, A-tobianus, A-dubius and Gymnammodytes semisquamatus by mitochondrial DNA restriction fragment patterns ' , Fisheries Research , vol. 36 , no. 1 , pp. 61-65 . https://doi.org/10.1016/S0165-7836(98)00081-2 |
op_relation |
https://pure.uhi.ac.uk/en/publications/762eeb45-7cd8-4535-afbb-f133689fccb9 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/S0165-7836(98)00081-2 |
container_title |
Fisheries Research |
container_volume |
36 |
container_issue |
1 |
container_start_page |
61 |
op_container_end_page |
65 |
_version_ |
1802647385072992256 |