UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen
The UV-susceptibility of zoospores of the lower sublittoral kelp Laminaria digitata was studied in the laboratory under varying fluence of spectral irradiance consisting of photosynthetically active radiation (PAR, 400-700 nm; = P), PAR + UV-A radiation (UV-A, 320-400 nm; = PA), and PAR + UV-A + UV-...
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2010
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Online Access: | https://pure.uhi.ac.uk/en/publications/7357a525-8fd5-4498-9480-0fae999fcae1 https://doi.org/10.1007/s00300-009-0733-z |
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ftuhipublicatio:oai:pure.atira.dk:publications/7357a525-8fd5-4498-9480-0fae999fcae1 2024-02-04T09:58:30+01:00 UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen Roleda, M Y Luder, U H Wiencke, C 2010 https://pure.uhi.ac.uk/en/publications/7357a525-8fd5-4498-9480-0fae999fcae1 https://doi.org/10.1007/s00300-009-0733-z eng eng info:eu-repo/semantics/restrictedAccess Roleda , M Y , Luder , U H & Wiencke , C 2010 , ' UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen ' , POLAR BIOL , vol. 33 , no. 5 , pp. 577-588 . https://doi.org/10.1007/s00300-009-0733-z SOUTH-PACIFIC KELPS Ecology EARLY-LIFE STAGES INDUCED DNA-DAMAGE Biodiversity Conservation ABSORBING COMPOUNDS ARCTIC LAMINARIALES ULTRAVIOLET-RADIATION PHOTOSYNTHETICALLY ACTIVE RADIATION NORTHERN-HEMISPHERE SACCORHIZA-DERMATODEA LESSONIA-TRABECULATA LAMINARIALES article 2010 ftuhipublicatio https://doi.org/10.1007/s00300-009-0733-z 2024-01-11T23:20:59Z The UV-susceptibility of zoospores of the lower sublittoral kelp Laminaria digitata was studied in the laboratory under varying fluence of spectral irradiance consisting of photosynthetically active radiation (PAR, 400-700 nm; = P), PAR + UV-A radiation (UV-A, 320-400 nm; = PA), and PAR + UV-A + UV-B radiation (UV-B, 280-320 nm; = PAB). In vivo absorption of phlorotannin, localisation of phlorotannin-containing physodes, structural changes, DNA damage and repair, photosynthesis and germination of zoospores were measured after exposure treatments and after 2-6 days of recovery in dim white light. Photodegradation of phlorotannins was observed after extended exposure to ultraviolet radiation (UVR). The UV-protective function of extra- and intracellular phlorotannins was, therefore, observed only after 8 h, but not after 16-h UVR exposure. The energetic cost of photoprotection may have caused the delay in ontogenic development of zoospores after 8-h exposure to PA and PAB treatment; longer exposure time corresponding to 16-h PA and PAB treatment eventually lead to cell degeneration at 6 days post-cultivation. The formation of cyclobutane-pyrimidine dimers (CPDs), as indicator of DNA damage, was not blocked by the UV-absorbing phlorotannins during the 16-h PAB exposure and the inability for DNA damage repair was likely responsible for low photosynthetic recovery and spore mortality. The higher sensitivity of L. digitata zoospores to UVR compared to other kelps such as Saccorhiza dermatodea and Alaria esculenta confirmed our hypothesis that the depth distribution of adult sporophytes in the field correlates to the sensitivity of their corresponding early life history stages to different stress factors in general and UVR in particular. Article in Journal/Newspaper Arctic Spitsbergen University of the Highlands and Islands: Research Database of UHI Arctic Pacific Polar Biology 33 5 577 588 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
SOUTH-PACIFIC KELPS Ecology EARLY-LIFE STAGES INDUCED DNA-DAMAGE Biodiversity Conservation ABSORBING COMPOUNDS ARCTIC LAMINARIALES ULTRAVIOLET-RADIATION PHOTOSYNTHETICALLY ACTIVE RADIATION NORTHERN-HEMISPHERE SACCORHIZA-DERMATODEA LESSONIA-TRABECULATA LAMINARIALES |
spellingShingle |
SOUTH-PACIFIC KELPS Ecology EARLY-LIFE STAGES INDUCED DNA-DAMAGE Biodiversity Conservation ABSORBING COMPOUNDS ARCTIC LAMINARIALES ULTRAVIOLET-RADIATION PHOTOSYNTHETICALLY ACTIVE RADIATION NORTHERN-HEMISPHERE SACCORHIZA-DERMATODEA LESSONIA-TRABECULATA LAMINARIALES Roleda, M Y Luder, U H Wiencke, C UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen |
topic_facet |
SOUTH-PACIFIC KELPS Ecology EARLY-LIFE STAGES INDUCED DNA-DAMAGE Biodiversity Conservation ABSORBING COMPOUNDS ARCTIC LAMINARIALES ULTRAVIOLET-RADIATION PHOTOSYNTHETICALLY ACTIVE RADIATION NORTHERN-HEMISPHERE SACCORHIZA-DERMATODEA LESSONIA-TRABECULATA LAMINARIALES |
description |
The UV-susceptibility of zoospores of the lower sublittoral kelp Laminaria digitata was studied in the laboratory under varying fluence of spectral irradiance consisting of photosynthetically active radiation (PAR, 400-700 nm; = P), PAR + UV-A radiation (UV-A, 320-400 nm; = PA), and PAR + UV-A + UV-B radiation (UV-B, 280-320 nm; = PAB). In vivo absorption of phlorotannin, localisation of phlorotannin-containing physodes, structural changes, DNA damage and repair, photosynthesis and germination of zoospores were measured after exposure treatments and after 2-6 days of recovery in dim white light. Photodegradation of phlorotannins was observed after extended exposure to ultraviolet radiation (UVR). The UV-protective function of extra- and intracellular phlorotannins was, therefore, observed only after 8 h, but not after 16-h UVR exposure. The energetic cost of photoprotection may have caused the delay in ontogenic development of zoospores after 8-h exposure to PA and PAB treatment; longer exposure time corresponding to 16-h PA and PAB treatment eventually lead to cell degeneration at 6 days post-cultivation. The formation of cyclobutane-pyrimidine dimers (CPDs), as indicator of DNA damage, was not blocked by the UV-absorbing phlorotannins during the 16-h PAB exposure and the inability for DNA damage repair was likely responsible for low photosynthetic recovery and spore mortality. The higher sensitivity of L. digitata zoospores to UVR compared to other kelps such as Saccorhiza dermatodea and Alaria esculenta confirmed our hypothesis that the depth distribution of adult sporophytes in the field correlates to the sensitivity of their corresponding early life history stages to different stress factors in general and UVR in particular. |
format |
Article in Journal/Newspaper |
author |
Roleda, M Y Luder, U H Wiencke, C |
author_facet |
Roleda, M Y Luder, U H Wiencke, C |
author_sort |
Roleda, M Y |
title |
UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen |
title_short |
UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen |
title_full |
UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen |
title_fullStr |
UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen |
title_full_unstemmed |
UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen |
title_sort |
uv-susceptibility of zoospores of the brown macroalga laminaria digitata from spitsbergen |
publishDate |
2010 |
url |
https://pure.uhi.ac.uk/en/publications/7357a525-8fd5-4498-9480-0fae999fcae1 https://doi.org/10.1007/s00300-009-0733-z |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
Roleda , M Y , Luder , U H & Wiencke , C 2010 , ' UV-susceptibility of zoospores of the brown macroalga Laminaria digitata from Spitsbergen ' , POLAR BIOL , vol. 33 , no. 5 , pp. 577-588 . https://doi.org/10.1007/s00300-009-0733-z |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00300-009-0733-z |
container_title |
Polar Biology |
container_volume |
33 |
container_issue |
5 |
container_start_page |
577 |
op_container_end_page |
588 |
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1789962942270144512 |