Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause
The marine copepod, Calanus finmarchicus, is a crucial component of the pelagic food web in the North Atlantic and peripheral seas where it is a major player in biogeochemical cycles and the productivity of commercially important fisheries. A key stage in its life cycle is the emergence of the pre-a...
Published in: | General and Comparative Endocrinology |
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Online Access: | https://pure.uhi.ac.uk/en/publications/351af538-4884-47db-8fcc-fe494010c686 https://doi.org/10.1016/j.ygcen.2013.04.002 |
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ftuhipublicatio:oai:pure.atira.dk:publications/351af538-4884-47db-8fcc-fe494010c686 2024-09-15T18:00:38+00:00 Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause Clark, Katie A. J. Brierley, Andrew S. Pond, David W. Smith, Valerie J. 2013-08-01 https://pure.uhi.ac.uk/en/publications/351af538-4884-47db-8fcc-fe494010c686 https://doi.org/10.1016/j.ygcen.2013.04.002 eng eng https://pure.uhi.ac.uk/en/publications/351af538-4884-47db-8fcc-fe494010c686 info:eu-repo/semantics/closedAccess Clark , K A J , Brierley , A S , Pond , D W & Smith , V J 2013 , ' Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment: An investigation of possible mediators of diapause ' , General and Comparative Endocrinology , vol. 189 , pp. 66-73 . https://doi.org/10.1016/j.ygcen.2013.04.002 Diapause Calanus finmarchicus Gene expression Retinoid X receptor Ecdysone receptor A-type allatostatin article 2013 ftuhipublicatio https://doi.org/10.1016/j.ygcen.2013.04.002 2024-06-24T23:48:36Z The marine copepod, Calanus finmarchicus, is a crucial component of the pelagic food web in the North Atlantic and peripheral seas where it is a major player in biogeochemical cycles and the productivity of commercially important fisheries. A key stage in its life cycle is the emergence of the pre-adult, copepodite developmental stage five (CV) from a period of overwintering dormancy, known as diapause. As is the case in many insect species, diapause is also likely to be under endocrine control in C. finmarchicus. To investigate the hormonal regulation of diapause behaviour of stage CV C. finmarchicus, the expression of three key genes: ecdysone receptor (EcR), retinoid X receptor (RXR) and an A-type allatostatin (A-type AST), were measured in specimens collected at monthly intervals from Loch Etive, a ca. 150 m deep sea loch on the west coast of Scotland, between June 2006 and May 2007. The full length RXR gene was cloned and sequenced from C. finmarchicus, and was found to share 49–53% total identity with equivalent genes encoding proteins from other crustaceans, and >80% identity in the DNA binding domain with other crustaceans, insects and vertebrates. EcR expression was least in December when the animals are expected to be in diapause, but began to increase in January, when the animals were terminating diapause. Concomittant with the rise in EcR in January was low expression of A-type AST and high expression of RXR. Article in Journal/Newspaper Calanus finmarchicus North Atlantic University of the Highlands and Islands: Research Database of UHI General and Comparative Endocrinology 189 66 73 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
Diapause Calanus finmarchicus Gene expression Retinoid X receptor Ecdysone receptor A-type allatostatin |
spellingShingle |
Diapause Calanus finmarchicus Gene expression Retinoid X receptor Ecdysone receptor A-type allatostatin Clark, Katie A. J. Brierley, Andrew S. Pond, David W. Smith, Valerie J. Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause |
topic_facet |
Diapause Calanus finmarchicus Gene expression Retinoid X receptor Ecdysone receptor A-type allatostatin |
description |
The marine copepod, Calanus finmarchicus, is a crucial component of the pelagic food web in the North Atlantic and peripheral seas where it is a major player in biogeochemical cycles and the productivity of commercially important fisheries. A key stage in its life cycle is the emergence of the pre-adult, copepodite developmental stage five (CV) from a period of overwintering dormancy, known as diapause. As is the case in many insect species, diapause is also likely to be under endocrine control in C. finmarchicus. To investigate the hormonal regulation of diapause behaviour of stage CV C. finmarchicus, the expression of three key genes: ecdysone receptor (EcR), retinoid X receptor (RXR) and an A-type allatostatin (A-type AST), were measured in specimens collected at monthly intervals from Loch Etive, a ca. 150 m deep sea loch on the west coast of Scotland, between June 2006 and May 2007. The full length RXR gene was cloned and sequenced from C. finmarchicus, and was found to share 49–53% total identity with equivalent genes encoding proteins from other crustaceans, and >80% identity in the DNA binding domain with other crustaceans, insects and vertebrates. EcR expression was least in December when the animals are expected to be in diapause, but began to increase in January, when the animals were terminating diapause. Concomittant with the rise in EcR in January was low expression of A-type AST and high expression of RXR. |
format |
Article in Journal/Newspaper |
author |
Clark, Katie A. J. Brierley, Andrew S. Pond, David W. Smith, Valerie J. |
author_facet |
Clark, Katie A. J. Brierley, Andrew S. Pond, David W. Smith, Valerie J. |
author_sort |
Clark, Katie A. J. |
title |
Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause |
title_short |
Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause |
title_full |
Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause |
title_fullStr |
Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause |
title_full_unstemmed |
Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment:: An investigation of possible mediators of diapause |
title_sort |
changes in seasonal expression patterns of ecdysone receptor, retinoid x receptor and an a-type allatostatin in the copepod, calanus finmarchicus, in a sea loch environment:: an investigation of possible mediators of diapause |
publishDate |
2013 |
url |
https://pure.uhi.ac.uk/en/publications/351af538-4884-47db-8fcc-fe494010c686 https://doi.org/10.1016/j.ygcen.2013.04.002 |
genre |
Calanus finmarchicus North Atlantic |
genre_facet |
Calanus finmarchicus North Atlantic |
op_source |
Clark , K A J , Brierley , A S , Pond , D W & Smith , V J 2013 , ' Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment: An investigation of possible mediators of diapause ' , General and Comparative Endocrinology , vol. 189 , pp. 66-73 . https://doi.org/10.1016/j.ygcen.2013.04.002 |
op_relation |
https://pure.uhi.ac.uk/en/publications/351af538-4884-47db-8fcc-fe494010c686 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.ygcen.2013.04.002 |
container_title |
General and Comparative Endocrinology |
container_volume |
189 |
container_start_page |
66 |
op_container_end_page |
73 |
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1810437819639791616 |