Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology
Coccolithoviruses (EhVs) are large, double-stranded DNA-containing viruses that infect the single-celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom-forming, calcifying, photoautotroph, E. huxleyi–EhV interactions play a key...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | https://pure.uhi.ac.uk/en/publications/1ebf1412-5781-4651-83aa-c281f6a61568 https://doi.org/10.1111/1462-2920.14633 https://pureadmin.uhi.ac.uk/ws/files/8180197/Nissimov_et_al._2018_Biochemical_diversity_of_sphingolipid_biosynthesis_as_a_driver_of_algal_virus_competitive_ecology_2_.pdf http://www.scopus.com/inward/record.url?scp=85066071964&partnerID=8YFLogxK http://www.scopus.com/inward/citedby.url?scp=85066071964&partnerID=8YFLogxK |
id |
ftuhipublicatio:oai:pure.atira.dk:publications/1ebf1412-5781-4651-83aa-c281f6a61568 |
---|---|
record_format |
openpolar |
spelling |
ftuhipublicatio:oai:pure.atira.dk:publications/1ebf1412-5781-4651-83aa-c281f6a61568 2024-01-28T10:07:32+01:00 Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J.L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A.S. Bidle, Kay D. 2019-06-02 application/pdf https://pure.uhi.ac.uk/en/publications/1ebf1412-5781-4651-83aa-c281f6a61568 https://doi.org/10.1111/1462-2920.14633 https://pureadmin.uhi.ac.uk/ws/files/8180197/Nissimov_et_al._2018_Biochemical_diversity_of_sphingolipid_biosynthesis_as_a_driver_of_algal_virus_competitive_ecology_2_.pdf http://www.scopus.com/inward/record.url?scp=85066071964&partnerID=8YFLogxK http://www.scopus.com/inward/citedby.url?scp=85066071964&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Nissimov , J I , Talmy , D , Haramaty , L , Fredricks , H F , Zelzion , E , Knowles , B , Eren , A M , Vandzura , R , Laber , C P , Schieler , B M , Johns , C T , More , K D , Coolen , M J L , Follows , M J , Bhattacharya , D , Van Mooy , B A S & Bidle , K D 2019 , ' Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology ' , Environmental Microbiology , vol. 21 , no. 6 , pp. 2182-2197 . https://doi.org/10.1111/1462-2920.14633 article 2019 ftuhipublicatio https://doi.org/10.1111/1462-2920.14633 2024-01-04T23:21:14Z Coccolithoviruses (EhVs) are large, double-stranded DNA-containing viruses that infect the single-celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom-forming, calcifying, photoautotroph, E. huxleyi–EhV interactions play a key role in oceanic carbon biogeochemistry. Virally-encoded glycosphingolipids (vGSLs) are virulence factors that are produced by the activity of virus-encoded serine palmitoyltransferase (SPT). Here, we characterize the dynamics, diversity and catalytic production of vGSLs in an array of EhV strains in relation to their SPT sequence composition and explore the hypothesis that they are a determinant of infectivity and host demise. vGSL production and diversity was positively correlated with increased virulence, virus replication rate and lytic infection dynamics in laboratory experiments, but they do not explain the success of less-virulent EhVs in natural EhV communities. The majority of EhV-derived SPT amplicon sequences associated with infected cells in the North Atlantic derived from slower infecting, less virulent EhVs. Our lab-, field- and mathematical model-based data and simulations support ecological scenarios whereby slow-infecting, less-virulent EhVs successfully compete in North Atlantic populations of E. huxleyi, through either the preferential removal of fast-infecting, virulent EhVs during active infection or by having access to a broader host range. Article in Journal/Newspaper North Atlantic University of the Highlands and Islands: Research Database of UHI Environmental Microbiology 21 6 2182 2197 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
description |
Coccolithoviruses (EhVs) are large, double-stranded DNA-containing viruses that infect the single-celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom-forming, calcifying, photoautotroph, E. huxleyi–EhV interactions play a key role in oceanic carbon biogeochemistry. Virally-encoded glycosphingolipids (vGSLs) are virulence factors that are produced by the activity of virus-encoded serine palmitoyltransferase (SPT). Here, we characterize the dynamics, diversity and catalytic production of vGSLs in an array of EhV strains in relation to their SPT sequence composition and explore the hypothesis that they are a determinant of infectivity and host demise. vGSL production and diversity was positively correlated with increased virulence, virus replication rate and lytic infection dynamics in laboratory experiments, but they do not explain the success of less-virulent EhVs in natural EhV communities. The majority of EhV-derived SPT amplicon sequences associated with infected cells in the North Atlantic derived from slower infecting, less virulent EhVs. Our lab-, field- and mathematical model-based data and simulations support ecological scenarios whereby slow-infecting, less-virulent EhVs successfully compete in North Atlantic populations of E. huxleyi, through either the preferential removal of fast-infecting, virulent EhVs during active infection or by having access to a broader host range. |
format |
Article in Journal/Newspaper |
author |
Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J.L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A.S. Bidle, Kay D. |
spellingShingle |
Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J.L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A.S. Bidle, Kay D. Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
author_facet |
Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J.L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A.S. Bidle, Kay D. |
author_sort |
Nissimov, Jozef I. |
title |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_short |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_full |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_fullStr |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_full_unstemmed |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_sort |
biochemical diversity of glycosphingolipid biosynthesis as a driver of coccolithovirus competitive ecology |
publishDate |
2019 |
url |
https://pure.uhi.ac.uk/en/publications/1ebf1412-5781-4651-83aa-c281f6a61568 https://doi.org/10.1111/1462-2920.14633 https://pureadmin.uhi.ac.uk/ws/files/8180197/Nissimov_et_al._2018_Biochemical_diversity_of_sphingolipid_biosynthesis_as_a_driver_of_algal_virus_competitive_ecology_2_.pdf http://www.scopus.com/inward/record.url?scp=85066071964&partnerID=8YFLogxK http://www.scopus.com/inward/citedby.url?scp=85066071964&partnerID=8YFLogxK |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Nissimov , J I , Talmy , D , Haramaty , L , Fredricks , H F , Zelzion , E , Knowles , B , Eren , A M , Vandzura , R , Laber , C P , Schieler , B M , Johns , C T , More , K D , Coolen , M J L , Follows , M J , Bhattacharya , D , Van Mooy , B A S & Bidle , K D 2019 , ' Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology ' , Environmental Microbiology , vol. 21 , no. 6 , pp. 2182-2197 . https://doi.org/10.1111/1462-2920.14633 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/1462-2920.14633 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
6 |
container_start_page |
2182 |
op_container_end_page |
2197 |
_version_ |
1789335356074622976 |