Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland
Shellfish aquaculture is a growing industry in Scotland, dominated by the production of the mussel Mytilus edulis, the native species. Recently the discovery of Mytilus galloprovincialis and Mytilus trossulus together with M. edulis and all 3 hybrids in cultivation in some Scottish sea lochs led to...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Language: | English |
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2008
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Online Access: | https://pure.uhi.ac.uk/en/publications/0ed859dc-4e89-44bb-8d44-46a5546aec90 https://doi.org/10.1016/j.jembe.2008.10.011 |
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ftuhipublicatio:oai:pure.atira.dk:publications/0ed859dc-4e89-44bb-8d44-46a5546aec90 2024-04-21T08:02:21+00:00 Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland Dias, P J Sollelis, L Cook, Elizabeth Piertney, S B Davies, Ian M Snow, M 2008 https://pure.uhi.ac.uk/en/publications/0ed859dc-4e89-44bb-8d44-46a5546aec90 https://doi.org/10.1016/j.jembe.2008.10.011 eng eng info:eu-repo/semantics/restrictedAccess Dias , P J , Sollelis , L , Cook , E , Piertney , S B , Davies , I M & Snow , M 2008 , ' Development of a real-time PCR assay for detection of Mytilus species specific alleles : Application to a sampling survey in Scotland ' , J EXP MAR BIOL ECOL , vol. 367 , no. 2 , pp. 253-258 . https://doi.org/10.1016/j.jembe.2008.10.011 COD GADUS-MORHUA Ecology GALLOPROVINCIALIS PLANKTON SAMPLES INDIGENOUS PERNA-PERNA Marine & Freshwater Biology SIMULTANEOUS IDENTIFICATION HYBRID ZONE BLUE MUSSELS EDULIS COMPLEX GENETIC-STRUCTURE MUSSEL MYTILUS article 2008 ftuhipublicatio https://doi.org/10.1016/j.jembe.2008.10.011 2024-03-28T01:05:59Z Shellfish aquaculture is a growing industry in Scotland, dominated by the production of the mussel Mytilus edulis, the native species. Recently the discovery of Mytilus galloprovincialis and Mytilus trossulus together with M. edulis and all 3 hybrids in cultivation in some Scottish sea lochs led to questions regarding the distribution of mussel species in Scotland. The establishment of an extensive sampling survey, involving the collection of mussels at 34 intertidal sites and 10 marinas around Scotland, motivated the development of a high-throughput method for identification of Mytilus alleles from samples. Three Taqman (R)-MGB probes and one set of primers were designed, based on the previously described Me 15/16 primers targeting the adhesive protein gene sequence, and samples were screened for the presence of M. edulis, M. galloprovincialis and M. trossulus alleles using real-time PCR. Mytilus edulis alleles were identified in samples from all 44 sites. Mytilus galloprovincialis alleles were found together with M. edulis alleles extensively in northern parts of the west and east coasts. Mytilus trossulus alleles were identified in samples from 6 sites in the west and southwest of Scotland. Because M. trossulus is generally undesirable in cultivation and therefore preventing the geographical spread of this species across Scotland is considered beneficial by the shellfish aquaculture industry, these 6 samples were further analysed for genotype frequencies using conventional PCR. Although distribution of the non-native species M. galloprovincialis and M. trossulus have proven to be more widespread than previously thought, there is no evidence from our study of either M. trossulus or M. galloprovincialis acting as an invasive species in Scotland. The real-time PCR method developed in this study has proven to be a rapid and effective tool for the identification of M. edulis, M. galloprovincialis and M. trossulus alleles from samples and should prove useful in future surveys, ecological or aquaculture management ... Article in Journal/Newspaper Gadus morhua University of the Highlands and Islands: Research Database of UHI Journal of Experimental Marine Biology and Ecology 367 2 253 258 |
institution |
Open Polar |
collection |
University of the Highlands and Islands: Research Database of UHI |
op_collection_id |
ftuhipublicatio |
language |
English |
topic |
COD GADUS-MORHUA Ecology GALLOPROVINCIALIS PLANKTON SAMPLES INDIGENOUS PERNA-PERNA Marine & Freshwater Biology SIMULTANEOUS IDENTIFICATION HYBRID ZONE BLUE MUSSELS EDULIS COMPLEX GENETIC-STRUCTURE MUSSEL MYTILUS |
spellingShingle |
COD GADUS-MORHUA Ecology GALLOPROVINCIALIS PLANKTON SAMPLES INDIGENOUS PERNA-PERNA Marine & Freshwater Biology SIMULTANEOUS IDENTIFICATION HYBRID ZONE BLUE MUSSELS EDULIS COMPLEX GENETIC-STRUCTURE MUSSEL MYTILUS Dias, P J Sollelis, L Cook, Elizabeth Piertney, S B Davies, Ian M Snow, M Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland |
topic_facet |
COD GADUS-MORHUA Ecology GALLOPROVINCIALIS PLANKTON SAMPLES INDIGENOUS PERNA-PERNA Marine & Freshwater Biology SIMULTANEOUS IDENTIFICATION HYBRID ZONE BLUE MUSSELS EDULIS COMPLEX GENETIC-STRUCTURE MUSSEL MYTILUS |
description |
Shellfish aquaculture is a growing industry in Scotland, dominated by the production of the mussel Mytilus edulis, the native species. Recently the discovery of Mytilus galloprovincialis and Mytilus trossulus together with M. edulis and all 3 hybrids in cultivation in some Scottish sea lochs led to questions regarding the distribution of mussel species in Scotland. The establishment of an extensive sampling survey, involving the collection of mussels at 34 intertidal sites and 10 marinas around Scotland, motivated the development of a high-throughput method for identification of Mytilus alleles from samples. Three Taqman (R)-MGB probes and one set of primers were designed, based on the previously described Me 15/16 primers targeting the adhesive protein gene sequence, and samples were screened for the presence of M. edulis, M. galloprovincialis and M. trossulus alleles using real-time PCR. Mytilus edulis alleles were identified in samples from all 44 sites. Mytilus galloprovincialis alleles were found together with M. edulis alleles extensively in northern parts of the west and east coasts. Mytilus trossulus alleles were identified in samples from 6 sites in the west and southwest of Scotland. Because M. trossulus is generally undesirable in cultivation and therefore preventing the geographical spread of this species across Scotland is considered beneficial by the shellfish aquaculture industry, these 6 samples were further analysed for genotype frequencies using conventional PCR. Although distribution of the non-native species M. galloprovincialis and M. trossulus have proven to be more widespread than previously thought, there is no evidence from our study of either M. trossulus or M. galloprovincialis acting as an invasive species in Scotland. The real-time PCR method developed in this study has proven to be a rapid and effective tool for the identification of M. edulis, M. galloprovincialis and M. trossulus alleles from samples and should prove useful in future surveys, ecological or aquaculture management ... |
format |
Article in Journal/Newspaper |
author |
Dias, P J Sollelis, L Cook, Elizabeth Piertney, S B Davies, Ian M Snow, M |
author_facet |
Dias, P J Sollelis, L Cook, Elizabeth Piertney, S B Davies, Ian M Snow, M |
author_sort |
Dias, P J |
title |
Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland |
title_short |
Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland |
title_full |
Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland |
title_fullStr |
Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland |
title_full_unstemmed |
Development of a real-time PCR assay for detection of Mytilus species specific alleles:Application to a sampling survey in Scotland |
title_sort |
development of a real-time pcr assay for detection of mytilus species specific alleles:application to a sampling survey in scotland |
publishDate |
2008 |
url |
https://pure.uhi.ac.uk/en/publications/0ed859dc-4e89-44bb-8d44-46a5546aec90 https://doi.org/10.1016/j.jembe.2008.10.011 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Dias , P J , Sollelis , L , Cook , E , Piertney , S B , Davies , I M & Snow , M 2008 , ' Development of a real-time PCR assay for detection of Mytilus species specific alleles : Application to a sampling survey in Scotland ' , J EXP MAR BIOL ECOL , vol. 367 , no. 2 , pp. 253-258 . https://doi.org/10.1016/j.jembe.2008.10.011 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.jembe.2008.10.011 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
367 |
container_issue |
2 |
container_start_page |
253 |
op_container_end_page |
258 |
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1796942543651340288 |