The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird
Despite the widely used application of standardized capture-handling protocols to collect blood and assess the physiological stress response, the actual sampling design (e.g., timing and the number of blood samples) often differs between studies, and the potential implications for the measured physi...
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ftuglasgow:oai:eprints.gla.ac.uk:258883 2023-05-15T15:34:42+02:00 The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird Huber, Nikolaus Mahr, Katharina Tóth, Zsófia Szarka, Endre Z. Çınar, Yusuf U. Salmón, Pablo Lendvai, Ádám Z. 2021-09-01 text https://eprints.gla.ac.uk/258883/ https://eprints.gla.ac.uk/258883/1/258883.pdf en eng Elsevier https://eprints.gla.ac.uk/258883/1/258883.pdf Huber, N., Mahr, K., Tóth, Z., Szarka, E. Z., Çınar, Y. U., Salmón, P. <http://eprints.gla.ac.uk/view/author/44695.html> and Lendvai, Á. Z. (2021) The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird. Physiology and Behavior <https://eprints.gla.ac.uk/view/journal_volume/Physiology_and_Behavior.html>, 238, 113488. (doi:10.1016/j.physbeh.2021.113488 <https://doi.org/10.1016/j.physbeh.2021.113488>) (PMID:34097972) cc_by_4 CC-BY Articles PeerReviewed 2021 ftuglasgow https://doi.org/10.1016/j.physbeh.2021.113488 2022-09-22T22:17:01Z Despite the widely used application of standardized capture-handling protocols to collect blood and assess the physiological stress response, the actual sampling design (e.g., timing and the number of blood samples) often differs between studies, and the potential implications for the measured physiological endpoints remain understudied. We, therefore experimentally tested the effects of repeated handling and multiple blood sampling on the stress response in wintering free-living great tits (Parus major). We modified a well-established sampling protocol of avian studies by adding either an additional blood sample or a “sham-manipulation” (i.e., handling associated with the blood sampling procedure without venepuncture), to disentangle the effects of handling stress and blood loss. We combined three different stress metrics along the endocrine-immune interface to investigate the acute short-term stress response: total corticosterone concentrations (Cort), the heterophil/lymphocyte ratio (H:L), and the Leucocyte Coping Capacity (LCC). Our study provided three key results: i) no relationship between Cort levels, LCC and H:L, confirming that these three parameters represent different physiological endpoints within the stress response; ii) contrasting dynamics in response to stress by the measured parameters and iii) no difference in physiological stress levels 30 min after capture due to one additional blood sampling or handling event. By optimising the sampling design, our results provide implications for animal welfare and planning experimental procedures on stress physiology in passerine species. Article in Journal/Newspaper Avian Studies University of Glasgow: Enlighten - Publications Parus ENVELOPE(3.950,3.950,-71.983,-71.983) Physiology & Behavior 238 113488 |
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Open Polar |
collection |
University of Glasgow: Enlighten - Publications |
op_collection_id |
ftuglasgow |
language |
English |
description |
Despite the widely used application of standardized capture-handling protocols to collect blood and assess the physiological stress response, the actual sampling design (e.g., timing and the number of blood samples) often differs between studies, and the potential implications for the measured physiological endpoints remain understudied. We, therefore experimentally tested the effects of repeated handling and multiple blood sampling on the stress response in wintering free-living great tits (Parus major). We modified a well-established sampling protocol of avian studies by adding either an additional blood sample or a “sham-manipulation” (i.e., handling associated with the blood sampling procedure without venepuncture), to disentangle the effects of handling stress and blood loss. We combined three different stress metrics along the endocrine-immune interface to investigate the acute short-term stress response: total corticosterone concentrations (Cort), the heterophil/lymphocyte ratio (H:L), and the Leucocyte Coping Capacity (LCC). Our study provided three key results: i) no relationship between Cort levels, LCC and H:L, confirming that these three parameters represent different physiological endpoints within the stress response; ii) contrasting dynamics in response to stress by the measured parameters and iii) no difference in physiological stress levels 30 min after capture due to one additional blood sampling or handling event. By optimising the sampling design, our results provide implications for animal welfare and planning experimental procedures on stress physiology in passerine species. |
format |
Article in Journal/Newspaper |
author |
Huber, Nikolaus Mahr, Katharina Tóth, Zsófia Szarka, Endre Z. Çınar, Yusuf U. Salmón, Pablo Lendvai, Ádám Z. |
spellingShingle |
Huber, Nikolaus Mahr, Katharina Tóth, Zsófia Szarka, Endre Z. Çınar, Yusuf U. Salmón, Pablo Lendvai, Ádám Z. The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
author_facet |
Huber, Nikolaus Mahr, Katharina Tóth, Zsófia Szarka, Endre Z. Çınar, Yusuf U. Salmón, Pablo Lendvai, Ádám Z. |
author_sort |
Huber, Nikolaus |
title |
The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
title_short |
The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
title_full |
The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
title_fullStr |
The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
title_full_unstemmed |
The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
title_sort |
stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://eprints.gla.ac.uk/258883/ https://eprints.gla.ac.uk/258883/1/258883.pdf |
long_lat |
ENVELOPE(3.950,3.950,-71.983,-71.983) |
geographic |
Parus |
geographic_facet |
Parus |
genre |
Avian Studies |
genre_facet |
Avian Studies |
op_relation |
https://eprints.gla.ac.uk/258883/1/258883.pdf Huber, N., Mahr, K., Tóth, Z., Szarka, E. Z., Çınar, Y. U., Salmón, P. <http://eprints.gla.ac.uk/view/author/44695.html> and Lendvai, Á. Z. (2021) The stressed bird in the hand: influence of sampling design on the physiological stress response in a free-living songbird. Physiology and Behavior <https://eprints.gla.ac.uk/view/journal_volume/Physiology_and_Behavior.html>, 238, 113488. (doi:10.1016/j.physbeh.2021.113488 <https://doi.org/10.1016/j.physbeh.2021.113488>) (PMID:34097972) |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.physbeh.2021.113488 |
container_title |
Physiology & Behavior |
container_volume |
238 |
container_start_page |
113488 |
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1766364998722912256 |