Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes
Tropospheric nitrate levels are predicted to increase throughout the 21st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and act...
Published in: | Environmental Microbiology Reports |
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Language: | English |
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Online Access: | http://eprints.gla.ac.uk/216927/ http://eprints.gla.ac.uk/216927/1/216927.pdf |
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ftuglasgow:oai:eprints.gla.ac.uk:216927 2023-05-15T16:27:51+02:00 Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. 2017-04 text http://eprints.gla.ac.uk/216927/ http://eprints.gla.ac.uk/216927/1/216927.pdf en eng Wiley http://eprints.gla.ac.uk/216927/1/216927.pdf Cameron, K. A. <http://eprints.gla.ac.uk/view/author/55427.html> , Stibal, M., Chrismas, N., Box, J. and Jacobsen, C. S. (2017) Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes. Environmental Microbiology Reports <http://eprints.gla.ac.uk/view/journal_volume/Environmental_Microbiology_Reports.html>, 9(2), pp. 144-150. (doi:10.1111/1758-2229.12510 <http://dx.doi.org/10.1111/1758-2229.12510>) (PMID:27943630) Articles PeerReviewed 2017 ftuglasgow https://doi.org/10.1111/1758-2229.12510 2021-04-08T22:09:06Z Tropospheric nitrate levels are predicted to increase throughout the 21st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic communities sampled from in situ nitrate fertilization plots on the GrIS surface. Nitrate concentrations were successfully elevated within sediment-filled meltwater pools, known as cryoconite holes; however, nitrate additions applied to surface ice did not persist. Estimated bulk and active cryoconite community cell abundance was unaltered by nitrate additions when compared to control holes using a quantitative PCR approach, and nitrate was found to have a minimal affect on the dominant 16S rRNA gene-based community composition. Together, these results indicate that sampled cryoconite communities were not nitrate limited at the time of sampling. Instead, temporal changes in biomass and community composition were more pronounced. As these in situ incubations were short (6 weeks), and the community composition across GrIS surface ice is highly variable, we suggest that further efforts should be considered to investigate the potential long-term impacts of increased nitrate across the GrIS. Article in Journal/Newspaper Greenland Ice Sheet University of Glasgow: Enlighten - Publications Greenland Environmental Microbiology Reports 9 2 144 150 |
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Open Polar |
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University of Glasgow: Enlighten - Publications |
op_collection_id |
ftuglasgow |
language |
English |
description |
Tropospheric nitrate levels are predicted to increase throughout the 21st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic communities sampled from in situ nitrate fertilization plots on the GrIS surface. Nitrate concentrations were successfully elevated within sediment-filled meltwater pools, known as cryoconite holes; however, nitrate additions applied to surface ice did not persist. Estimated bulk and active cryoconite community cell abundance was unaltered by nitrate additions when compared to control holes using a quantitative PCR approach, and nitrate was found to have a minimal affect on the dominant 16S rRNA gene-based community composition. Together, these results indicate that sampled cryoconite communities were not nitrate limited at the time of sampling. Instead, temporal changes in biomass and community composition were more pronounced. As these in situ incubations were short (6 weeks), and the community composition across GrIS surface ice is highly variable, we suggest that further efforts should be considered to investigate the potential long-term impacts of increased nitrate across the GrIS. |
format |
Article in Journal/Newspaper |
author |
Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. |
spellingShingle |
Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes |
author_facet |
Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. |
author_sort |
Cameron, Karen A. |
title |
Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes |
title_short |
Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes |
title_full |
Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes |
title_fullStr |
Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes |
title_full_unstemmed |
Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes |
title_sort |
nitrate addition has minimal short-term impacts on greenland ice sheet supraglacial prokaryotes |
publisher |
Wiley |
publishDate |
2017 |
url |
http://eprints.gla.ac.uk/216927/ http://eprints.gla.ac.uk/216927/1/216927.pdf |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_relation |
http://eprints.gla.ac.uk/216927/1/216927.pdf Cameron, K. A. <http://eprints.gla.ac.uk/view/author/55427.html> , Stibal, M., Chrismas, N., Box, J. and Jacobsen, C. S. (2017) Nitrate addition has minimal short-term impacts on Greenland ice sheet supraglacial prokaryotes. Environmental Microbiology Reports <http://eprints.gla.ac.uk/view/journal_volume/Environmental_Microbiology_Reports.html>, 9(2), pp. 144-150. (doi:10.1111/1758-2229.12510 <http://dx.doi.org/10.1111/1758-2229.12510>) (PMID:27943630) |
op_doi |
https://doi.org/10.1111/1758-2229.12510 |
container_title |
Environmental Microbiology Reports |
container_volume |
9 |
container_issue |
2 |
container_start_page |
144 |
op_container_end_page |
150 |
_version_ |
1766017404542910464 |