The new species Brucella microti replicates in macrophages and causes death in murine models of infection
Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was an...
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ftuglasgow:oai:eprints.gla.ac.uk:203230 2023-05-15T15:56:36+02:00 The new species Brucella microti replicates in macrophages and causes death in murine models of infection Jiménez de Bagüés, María P. Ouahrani‐Bettache, Safia Quintana, Juan F. Mitjana, Olga Hanna, Nabil Bessoles, Stéphanie Sanchez, Françoise Scholz, Holger C. Lafont, Virginie Köhler, Stephan Occhialini, Alessandra 2010-07-01 https://eprints.gla.ac.uk/203230/ unknown Infectious Diseases Society of America Jiménez de Bagüés, M. P. et al. (2010) The new species Brucella microti replicates in macrophages and causes death in murine models of infection. Journal of Infectious Diseases <https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Infectious_Diseases.html>, 202(1), pp. 3-10. (doi:10.1086/653084 <https://doi.org/10.1086/653084>) (PMID:20497040) Articles PeerReviewed 2010 ftuglasgow https://doi.org/10.1086/653084 2022-09-22T22:15:33Z Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results.B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. In experimental cellular and murine infections, B. microti exhibited a high pathogenic potential, compared with other Brucella species. Article in Journal/Newspaper Common vole University of Glasgow: Enlighten - Publications The Journal of Infectious Diseases 202 1 3 10 |
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University of Glasgow: Enlighten - Publications |
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Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results.B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. In experimental cellular and murine infections, B. microti exhibited a high pathogenic potential, compared with other Brucella species. |
format |
Article in Journal/Newspaper |
author |
Jiménez de Bagüés, María P. Ouahrani‐Bettache, Safia Quintana, Juan F. Mitjana, Olga Hanna, Nabil Bessoles, Stéphanie Sanchez, Françoise Scholz, Holger C. Lafont, Virginie Köhler, Stephan Occhialini, Alessandra |
spellingShingle |
Jiménez de Bagüés, María P. Ouahrani‐Bettache, Safia Quintana, Juan F. Mitjana, Olga Hanna, Nabil Bessoles, Stéphanie Sanchez, Françoise Scholz, Holger C. Lafont, Virginie Köhler, Stephan Occhialini, Alessandra The new species Brucella microti replicates in macrophages and causes death in murine models of infection |
author_facet |
Jiménez de Bagüés, María P. Ouahrani‐Bettache, Safia Quintana, Juan F. Mitjana, Olga Hanna, Nabil Bessoles, Stéphanie Sanchez, Françoise Scholz, Holger C. Lafont, Virginie Köhler, Stephan Occhialini, Alessandra |
author_sort |
Jiménez de Bagüés, María P. |
title |
The new species Brucella microti replicates in macrophages and causes death in murine models of infection |
title_short |
The new species Brucella microti replicates in macrophages and causes death in murine models of infection |
title_full |
The new species Brucella microti replicates in macrophages and causes death in murine models of infection |
title_fullStr |
The new species Brucella microti replicates in macrophages and causes death in murine models of infection |
title_full_unstemmed |
The new species Brucella microti replicates in macrophages and causes death in murine models of infection |
title_sort |
new species brucella microti replicates in macrophages and causes death in murine models of infection |
publisher |
Infectious Diseases Society of America |
publishDate |
2010 |
url |
https://eprints.gla.ac.uk/203230/ |
genre |
Common vole |
genre_facet |
Common vole |
op_relation |
Jiménez de Bagüés, M. P. et al. (2010) The new species Brucella microti replicates in macrophages and causes death in murine models of infection. Journal of Infectious Diseases <https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Infectious_Diseases.html>, 202(1), pp. 3-10. (doi:10.1086/653084 <https://doi.org/10.1086/653084>) (PMID:20497040) |
op_doi |
https://doi.org/10.1086/653084 |
container_title |
The Journal of Infectious Diseases |
container_volume |
202 |
container_issue |
1 |
container_start_page |
3 |
op_container_end_page |
10 |
_version_ |
1766391992698273792 |