Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects
The scuticociliatosis is a very serious disease that affects the cultured turbot, and whose causal agent is the anphizoic and marine euryhaline ciliate Philasterides dicentrarchi. Several protozoans possess acidic organelles that contain high concentrations of pyrophosphate (PPi), Ca2+ and other ele...
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ftuglasgow:oai:eprints.gla.ac.uk:149295 2023-05-15T18:41:11+02:00 Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects Mallo, Natalia Lamas, Jesús de Felipe, Ana-Paula Sueiro, Rosa-Ana Fontenla, Francisco Leiro, José-Manuel 2016-10 http://eprints.gla.ac.uk/149295/ unknown Elsevier Mallo, N. <http://eprints.gla.ac.uk/view/author/36521.html>, Lamas, J., de Felipe, A.-P., Sueiro, R.-A., Fontenla, F. and Leiro, J.-M. (2016) Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects. Experimental Parasitology <http://eprints.gla.ac.uk/view/journal_volume/Experimental_Parasitology.html>, 169, pp. 59-68. (doi:10.1016/j.exppara.2016.07.012 <http://dx.doi.org/10.1016/j.exppara.2016.07.012>) (PMID:27480055) Articles PeerReviewed 2016 ftuglasgow https://doi.org/10.1016/j.exppara.2016.07.012 2020-01-10T01:30:01Z The scuticociliatosis is a very serious disease that affects the cultured turbot, and whose causal agent is the anphizoic and marine euryhaline ciliate Philasterides dicentrarchi. Several protozoans possess acidic organelles that contain high concentrations of pyrophosphate (PPi), Ca2+ and other elements with essential roles in vesicular trafficking, pH homeostasis and osmoregulation. P. dicentrarchi possesses a pyrophosphatase (H+-PPase) that pumps H+ through the membranes of vacuolar and alveolar sacs. These compartments share common features with the acidocalcisomes described in other parasitic protozoa (e.g. acid content and Ca2+ storage). We evaluated the effects of Ca2+ and ATP on H +-PPase activity in this ciliate and analyzed their role in maintaining intracellular pH homeostasis and osmoregulation, by the addition of PPi and inorganic molecules that affect osmolarity. Addition of PPi led to acidification of the intracellular compartments, while the addition of ATP, CaCl2 and bisphosphonates analogous of PPi and Ca2+ metabolism regulators led to alkalinization and a decrease in H+-PPase expression in trophozoites. Addition of NaCl led to proton release, intracellular Ca2+ accumulation and downregulation of H+-PPase expression. We conclude that the regulation of the acidification of intracellular compartments may be essential for maintaining the intracellular pH homeostasis necessary for survival of ciliates and their adaptation to salt stress, which they will presumably face during the endoparasitic phase, in which the salinity levels are lower than in their natural environment. Article in Journal/Newspaper Turbot University of Glasgow: Enlighten - Publications Experimental Parasitology 169 59 68 |
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University of Glasgow: Enlighten - Publications |
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ftuglasgow |
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description |
The scuticociliatosis is a very serious disease that affects the cultured turbot, and whose causal agent is the anphizoic and marine euryhaline ciliate Philasterides dicentrarchi. Several protozoans possess acidic organelles that contain high concentrations of pyrophosphate (PPi), Ca2+ and other elements with essential roles in vesicular trafficking, pH homeostasis and osmoregulation. P. dicentrarchi possesses a pyrophosphatase (H+-PPase) that pumps H+ through the membranes of vacuolar and alveolar sacs. These compartments share common features with the acidocalcisomes described in other parasitic protozoa (e.g. acid content and Ca2+ storage). We evaluated the effects of Ca2+ and ATP on H +-PPase activity in this ciliate and analyzed their role in maintaining intracellular pH homeostasis and osmoregulation, by the addition of PPi and inorganic molecules that affect osmolarity. Addition of PPi led to acidification of the intracellular compartments, while the addition of ATP, CaCl2 and bisphosphonates analogous of PPi and Ca2+ metabolism regulators led to alkalinization and a decrease in H+-PPase expression in trophozoites. Addition of NaCl led to proton release, intracellular Ca2+ accumulation and downregulation of H+-PPase expression. We conclude that the regulation of the acidification of intracellular compartments may be essential for maintaining the intracellular pH homeostasis necessary for survival of ciliates and their adaptation to salt stress, which they will presumably face during the endoparasitic phase, in which the salinity levels are lower than in their natural environment. |
format |
Article in Journal/Newspaper |
author |
Mallo, Natalia Lamas, Jesús de Felipe, Ana-Paula Sueiro, Rosa-Ana Fontenla, Francisco Leiro, José-Manuel |
spellingShingle |
Mallo, Natalia Lamas, Jesús de Felipe, Ana-Paula Sueiro, Rosa-Ana Fontenla, Francisco Leiro, José-Manuel Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects |
author_facet |
Mallo, Natalia Lamas, Jesús de Felipe, Ana-Paula Sueiro, Rosa-Ana Fontenla, Francisco Leiro, José-Manuel |
author_sort |
Mallo, Natalia |
title |
Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects |
title_short |
Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects |
title_full |
Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects |
title_fullStr |
Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects |
title_full_unstemmed |
Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects |
title_sort |
role of h+-pyrophosphatase activity in the regulation of intracellular ph in a scuticociliate parasite of turbot: physiological effects |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://eprints.gla.ac.uk/149295/ |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
Mallo, N. <http://eprints.gla.ac.uk/view/author/36521.html>, Lamas, J., de Felipe, A.-P., Sueiro, R.-A., Fontenla, F. and Leiro, J.-M. (2016) Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects. Experimental Parasitology <http://eprints.gla.ac.uk/view/journal_volume/Experimental_Parasitology.html>, 169, pp. 59-68. (doi:10.1016/j.exppara.2016.07.012 <http://dx.doi.org/10.1016/j.exppara.2016.07.012>) (PMID:27480055) |
op_doi |
https://doi.org/10.1016/j.exppara.2016.07.012 |
container_title |
Experimental Parasitology |
container_volume |
169 |
container_start_page |
59 |
op_container_end_page |
68 |
_version_ |
1766230672722100224 |