Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching
Mass staining of decapod females carrying eggs, with subsequent identification of hatched larvae in the environment, is a research tool with great potential for field ecologists wishing to track the movements of larvae. For this to be achieved, however, numerous requirements must be met. These inclu...
Published in: | Biotechnic & Histochemistry |
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Taylor & Francis
2012
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ftuglasgow:oai:eprints.gla.ac.uk:135309 2023-05-15T16:08:48+02:00 Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching Øresland, V. Horobin, R.W. 2012 http://eprints.gla.ac.uk/135309/ unknown Taylor & Francis Øresland, V. and Horobin, R.W. <http://eprints.gla.ac.uk/view/author/12026.html> (2012) Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching. Biotechnic and Histochemistry <http://eprints.gla.ac.uk/view/journal_volume/Biotechnic_and_Histochemistry.html>, 87(3), pp. 229-234. (doi:10.3109/10520295.2011.639718 <http://dx.doi.org/10.3109/10520295.2011.639718>) (PMID:22149046) Articles PeerReviewed 2012 ftuglasgow https://doi.org/10.3109/10520295.2011.639718 2020-01-10T01:18:22Z Mass staining of decapod females carrying eggs, with subsequent identification of hatched larvae in the environment, is a research tool with great potential for field ecologists wishing to track the movements of larvae. For this to be achieved, however, numerous requirements must be met. These include adequate dye solubility, short staining time, dye penetration through different tissues, dye retention within the organism, absence of toxic and behavioral effects, low visibility to predators of stained larvae, no loss of staining owing to preservatives and low cost. The dye, neutral red, appears to meet most of these requirements. This dye was used in aliquots of 0.7 g/770 ml seawater applied to the females of Norway lobster (Nephrops norvegicus) and European lobster (Homarus gammarus) for 10 min. This procedure stained lobster eggs and embryos so that hatched larvae could be distinguished easily by fluorescence microscopy from larvae that hatched from unstained eggs. Stained larvae that were preserved in 4% formaldehyde in seawater were still stained after 1 year. Larvae should not come in contact with ethanol, because it extracts the dye rapidly. Article in Journal/Newspaper European lobster Homarus gammarus University of Glasgow: Enlighten - Publications Norway Biotechnic & Histochemistry 87 3 229 234 |
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Open Polar |
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University of Glasgow: Enlighten - Publications |
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ftuglasgow |
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description |
Mass staining of decapod females carrying eggs, with subsequent identification of hatched larvae in the environment, is a research tool with great potential for field ecologists wishing to track the movements of larvae. For this to be achieved, however, numerous requirements must be met. These include adequate dye solubility, short staining time, dye penetration through different tissues, dye retention within the organism, absence of toxic and behavioral effects, low visibility to predators of stained larvae, no loss of staining owing to preservatives and low cost. The dye, neutral red, appears to meet most of these requirements. This dye was used in aliquots of 0.7 g/770 ml seawater applied to the females of Norway lobster (Nephrops norvegicus) and European lobster (Homarus gammarus) for 10 min. This procedure stained lobster eggs and embryos so that hatched larvae could be distinguished easily by fluorescence microscopy from larvae that hatched from unstained eggs. Stained larvae that were preserved in 4% formaldehyde in seawater were still stained after 1 year. Larvae should not come in contact with ethanol, because it extracts the dye rapidly. |
format |
Article in Journal/Newspaper |
author |
Øresland, V. Horobin, R.W. |
spellingShingle |
Øresland, V. Horobin, R.W. Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
author_facet |
Øresland, V. Horobin, R.W. |
author_sort |
Øresland, V. |
title |
Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
title_short |
Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
title_full |
Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
title_fullStr |
Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
title_full_unstemmed |
Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
title_sort |
tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching |
publisher |
Taylor & Francis |
publishDate |
2012 |
url |
http://eprints.gla.ac.uk/135309/ |
geographic |
Norway |
geographic_facet |
Norway |
genre |
European lobster Homarus gammarus |
genre_facet |
European lobster Homarus gammarus |
op_relation |
Øresland, V. and Horobin, R.W. <http://eprints.gla.ac.uk/view/author/12026.html> (2012) Tracking living decapod larvae: mass staining of eggs with neutral red prior to hatching. Biotechnic and Histochemistry <http://eprints.gla.ac.uk/view/journal_volume/Biotechnic_and_Histochemistry.html>, 87(3), pp. 229-234. (doi:10.3109/10520295.2011.639718 <http://dx.doi.org/10.3109/10520295.2011.639718>) (PMID:22149046) |
op_doi |
https://doi.org/10.3109/10520295.2011.639718 |
container_title |
Biotechnic & Histochemistry |
container_volume |
87 |
container_issue |
3 |
container_start_page |
229 |
op_container_end_page |
234 |
_version_ |
1766404822367469568 |