Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3)
While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on-farm qualitative test and an in-laboratory quantitative assay for health assessments in aquacult...
Published in: | Journal of Fish Diseases |
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ftuglasgow:oai:eprints.gla.ac.uk:130551 2023-05-15T15:31:19+02:00 Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) Braceland, M. Tinsley, J. Cockerill, D. Bickerdike, R. McLoughlin, M.F. Eckersall, P.D. 2017-08 text https://eprints.gla.ac.uk/130551/ https://eprints.gla.ac.uk/130551/7/130551.pdf en eng Wiley https://eprints.gla.ac.uk/130551/7/130551.pdf Braceland, M. <http://eprints.gla.ac.uk/view/author/33514.html>, Tinsley, J., Cockerill, D., Bickerdike, R., McLoughlin, M.F. and Eckersall, P.D. <http://eprints.gla.ac.uk/view/author/10160.html> (2017) Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3). Journal of Fish Diseases <https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Fish_Diseases.html>, 40(8), pp. 1077-1087. (doi:10.1111/jfd.12587 <https://doi.org/10.1111/jfd.12587>) (PMID:27905123) (PMCID:PMC5516131) cc_by_4 CC-BY Articles PeerReviewed 2017 ftuglasgow https://doi.org/10.1111/jfd.12587 2022-09-22T22:13:15Z While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on-farm qualitative test and an in-laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ340). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ340 in infected fish rose from undetectable to a maximum at 6 weeks post-infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Glasgow: Enlighten - Publications Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of Fish Diseases 40 8 1077 1087 |
institution |
Open Polar |
collection |
University of Glasgow: Enlighten - Publications |
op_collection_id |
ftuglasgow |
language |
English |
description |
While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on-farm qualitative test and an in-laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ340). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ340 in infected fish rose from undetectable to a maximum at 6 weeks post-infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon. |
format |
Article in Journal/Newspaper |
author |
Braceland, M. Tinsley, J. Cockerill, D. Bickerdike, R. McLoughlin, M.F. Eckersall, P.D. |
spellingShingle |
Braceland, M. Tinsley, J. Cockerill, D. Bickerdike, R. McLoughlin, M.F. Eckersall, P.D. Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) |
author_facet |
Braceland, M. Tinsley, J. Cockerill, D. Bickerdike, R. McLoughlin, M.F. Eckersall, P.D. |
author_sort |
Braceland, M. |
title |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) |
title_short |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) |
title_full |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) |
title_fullStr |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) |
title_full_unstemmed |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3) |
title_sort |
selective precipitation reaction: a novel diagnostic test for tissue pathology in atlantic salmon, salmo salar, infected with salmonid alpha-virus (sav3) |
publisher |
Wiley |
publishDate |
2017 |
url |
https://eprints.gla.ac.uk/130551/ https://eprints.gla.ac.uk/130551/7/130551.pdf |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://eprints.gla.ac.uk/130551/7/130551.pdf Braceland, M. <http://eprints.gla.ac.uk/view/author/33514.html>, Tinsley, J., Cockerill, D., Bickerdike, R., McLoughlin, M.F. and Eckersall, P.D. <http://eprints.gla.ac.uk/view/author/10160.html> (2017) Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3). Journal of Fish Diseases <https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Fish_Diseases.html>, 40(8), pp. 1077-1087. (doi:10.1111/jfd.12587 <https://doi.org/10.1111/jfd.12587>) (PMID:27905123) (PMCID:PMC5516131) |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/jfd.12587 |
container_title |
Journal of Fish Diseases |
container_volume |
40 |
container_issue |
8 |
container_start_page |
1077 |
op_container_end_page |
1087 |
_version_ |
1766361814477570048 |