Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias)
The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular an...
Published in: | Developmental & Comparative Immunology |
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ftuglasgow:oai:eprints.gla.ac.uk:129667 2023-05-15T18:51:06+02:00 Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) Smith, Lauren E. Crouch, Kathryn Cao, Wei Müller, Mischa R. Wu, Leeying Steven, John Lee, Michael Liang, Musen Flajnik, Martin F. Shih, Heather H. Barelle, Caroline J. Paulsen, Janet Gill, Davinder S. Dooley, Helen 2012-04 http://eprints.gla.ac.uk/129667/ unknown Elsevier Smith, L. E. et al. (2012) Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias). Developmental and Comparative Immunology <http://eprints.gla.ac.uk/view/journal_volume/Developmental_and_Comparative_Immunology.html>, 36(4), pp. 665-679. (doi:10.1016/j.dci.2011.10.007 <http://dx.doi.org/10.1016/j.dci.2011.10.007>) (PMID:22040740) QH301 Biology Articles PeerReviewed 2012 ftuglasgow https://doi.org/10.1016/j.dci.2011.10.007 2020-01-10T01:13:31Z The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein levels. Despite the presence of hundreds of IgM clusters in this species the serum levels of this isotype are comparatively low. However, analysis of cDNA sequences and serum protein suggests microheterogeneity in the IgM heavy chains and supports the proposal that different clusters are preferentially used in the two forms (monomer or pentamer) of this isotype. We also found that the IgNAR isotype in this species exists in a previously unknown multimeric format in serum. Finally, we identified a new form of the IgW isotype (the shark IgD orthologue), in which the leader is spliced directly to the first constant domain, resulting in a molecule lacking an antigen-binding domain. Article in Journal/Newspaper spiny dogfish Squalus acanthias University of Glasgow: Enlighten - Publications Developmental & Comparative Immunology 36 4 665 679 |
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University of Glasgow: Enlighten - Publications |
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topic |
QH301 Biology |
spellingShingle |
QH301 Biology Smith, Lauren E. Crouch, Kathryn Cao, Wei Müller, Mischa R. Wu, Leeying Steven, John Lee, Michael Liang, Musen Flajnik, Martin F. Shih, Heather H. Barelle, Caroline J. Paulsen, Janet Gill, Davinder S. Dooley, Helen Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) |
topic_facet |
QH301 Biology |
description |
The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein levels. Despite the presence of hundreds of IgM clusters in this species the serum levels of this isotype are comparatively low. However, analysis of cDNA sequences and serum protein suggests microheterogeneity in the IgM heavy chains and supports the proposal that different clusters are preferentially used in the two forms (monomer or pentamer) of this isotype. We also found that the IgNAR isotype in this species exists in a previously unknown multimeric format in serum. Finally, we identified a new form of the IgW isotype (the shark IgD orthologue), in which the leader is spliced directly to the first constant domain, resulting in a molecule lacking an antigen-binding domain. |
format |
Article in Journal/Newspaper |
author |
Smith, Lauren E. Crouch, Kathryn Cao, Wei Müller, Mischa R. Wu, Leeying Steven, John Lee, Michael Liang, Musen Flajnik, Martin F. Shih, Heather H. Barelle, Caroline J. Paulsen, Janet Gill, Davinder S. Dooley, Helen |
author_facet |
Smith, Lauren E. Crouch, Kathryn Cao, Wei Müller, Mischa R. Wu, Leeying Steven, John Lee, Michael Liang, Musen Flajnik, Martin F. Shih, Heather H. Barelle, Caroline J. Paulsen, Janet Gill, Davinder S. Dooley, Helen |
author_sort |
Smith, Lauren E. |
title |
Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) |
title_short |
Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) |
title_full |
Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) |
title_fullStr |
Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) |
title_full_unstemmed |
Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias) |
title_sort |
characterization of the immunoglobulin repertoire of the spiny dogfish (squalus acanthias) |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://eprints.gla.ac.uk/129667/ |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
Smith, L. E. et al. (2012) Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias). Developmental and Comparative Immunology <http://eprints.gla.ac.uk/view/journal_volume/Developmental_and_Comparative_Immunology.html>, 36(4), pp. 665-679. (doi:10.1016/j.dci.2011.10.007 <http://dx.doi.org/10.1016/j.dci.2011.10.007>) (PMID:22040740) |
op_doi |
https://doi.org/10.1016/j.dci.2011.10.007 |
container_title |
Developmental & Comparative Immunology |
container_volume |
36 |
container_issue |
4 |
container_start_page |
665 |
op_container_end_page |
679 |
_version_ |
1766244898580725760 |