Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids
Improved testing and remediation procedures for sites contaminated with petroleum hydrocarbons are a priority in remote cold regions such as Antarctica, where costs are higher and remediation times are longer. Isoprenoid/n-alkane ratios are commonly used to determine the extent of biodegradation at...
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American Chemical Society
2007
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ftuglasgow:oai:eprints.gla.ac.uk:116956 2023-05-15T14:04:48+02:00 Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids Mcintyre, Cameron P. Harvey, Paul McA. Ferguson, Susan H. Wressnig, Anna M. Volk, Herbert George, Simon C. Snape, Ian 2007 http://eprints.gla.ac.uk/116956/ unknown American Chemical Society Mcintyre, C. P. <http://eprints.gla.ac.uk/view/author/34583.html> , Harvey, P. M., Ferguson, S. H., Wressnig, A. M., Volk, H., George, S. C. and Snape, I. (2007) Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids. Environmental Science and Technology <http://eprints.gla.ac.uk/view/journal_volume/Environmental_Science_and_Technology.html>, 41(7), pp. 2452-2458. (doi:10.1021/es0621288 <http://dx.doi.org/10.1021/es0621288>) (PMID:17438799) Articles PeerReviewed 2007 ftuglasgow https://doi.org/10.1021/es0621288 2020-01-10T01:04:41Z Improved testing and remediation procedures for sites contaminated with petroleum hydrocarbons are a priority in remote cold regions such as Antarctica, where costs are higher and remediation times are longer. Isoprenoid/n-alkane ratios are commonly used to determine the extent of biodegradation at low levels but are not useful once the n-alkanes have been removed. This study demonstrates how the diastereomers of the acyclic isoprenoids can be used to determine the extent of biodegradation in moderately biodegraded fuel in soils from a bioremediation trial at Casey Station, Antarctica. The biological diaster eomers of pristane (meso; RS = SR) are depleted more rapidly during moderate biodegradation than the geological or mature diastereomers (RR and SS), and thus, the ratio of pristane diastereomers can determine the level of biodegradation. The statistical difference among mean diastereomer ratios for samples grouped according to the biodegradation scale and pristane/phytane ratios was highly significant. The ratios of norpristane and phytane diastereomers also change with biodegradation in a similar fashion, and different levels of sensitivity exist for each. Additional benefits are that the method can be performed on conventional gas chromatographs by non-specialist chemists and that the ratios are independent of evaporation and do not necessarily require a non-biodegraded reference (T0) sample. This study details a simple alternative method for determining the extent of biodegradation of fuels at moderate levels that can be applied to a wide range of petroleum products. Article in Journal/Newspaper Antarc* Antarctica University of Glasgow: Enlighten - Publications Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) Environmental Science & Technology 41 7 2452 2458 |
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University of Glasgow: Enlighten - Publications |
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ftuglasgow |
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description |
Improved testing and remediation procedures for sites contaminated with petroleum hydrocarbons are a priority in remote cold regions such as Antarctica, where costs are higher and remediation times are longer. Isoprenoid/n-alkane ratios are commonly used to determine the extent of biodegradation at low levels but are not useful once the n-alkanes have been removed. This study demonstrates how the diastereomers of the acyclic isoprenoids can be used to determine the extent of biodegradation in moderately biodegraded fuel in soils from a bioremediation trial at Casey Station, Antarctica. The biological diaster eomers of pristane (meso; RS = SR) are depleted more rapidly during moderate biodegradation than the geological or mature diastereomers (RR and SS), and thus, the ratio of pristane diastereomers can determine the level of biodegradation. The statistical difference among mean diastereomer ratios for samples grouped according to the biodegradation scale and pristane/phytane ratios was highly significant. The ratios of norpristane and phytane diastereomers also change with biodegradation in a similar fashion, and different levels of sensitivity exist for each. Additional benefits are that the method can be performed on conventional gas chromatographs by non-specialist chemists and that the ratios are independent of evaporation and do not necessarily require a non-biodegraded reference (T0) sample. This study details a simple alternative method for determining the extent of biodegradation of fuels at moderate levels that can be applied to a wide range of petroleum products. |
format |
Article in Journal/Newspaper |
author |
Mcintyre, Cameron P. Harvey, Paul McA. Ferguson, Susan H. Wressnig, Anna M. Volk, Herbert George, Simon C. Snape, Ian |
spellingShingle |
Mcintyre, Cameron P. Harvey, Paul McA. Ferguson, Susan H. Wressnig, Anna M. Volk, Herbert George, Simon C. Snape, Ian Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
author_facet |
Mcintyre, Cameron P. Harvey, Paul McA. Ferguson, Susan H. Wressnig, Anna M. Volk, Herbert George, Simon C. Snape, Ian |
author_sort |
Mcintyre, Cameron P. |
title |
Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
title_short |
Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
title_full |
Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
title_fullStr |
Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
title_full_unstemmed |
Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
title_sort |
determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids |
publisher |
American Chemical Society |
publishDate |
2007 |
url |
http://eprints.gla.ac.uk/116956/ |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) |
geographic |
Casey Station |
geographic_facet |
Casey Station |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Mcintyre, C. P. <http://eprints.gla.ac.uk/view/author/34583.html> , Harvey, P. M., Ferguson, S. H., Wressnig, A. M., Volk, H., George, S. C. and Snape, I. (2007) Determining the extent of biodegradation of fuels using the diastereomers of acyclic isoprenoids. Environmental Science and Technology <http://eprints.gla.ac.uk/view/journal_volume/Environmental_Science_and_Technology.html>, 41(7), pp. 2452-2458. (doi:10.1021/es0621288 <http://dx.doi.org/10.1021/es0621288>) (PMID:17438799) |
op_doi |
https://doi.org/10.1021/es0621288 |
container_title |
Environmental Science & Technology |
container_volume |
41 |
container_issue |
7 |
container_start_page |
2452 |
op_container_end_page |
2458 |
_version_ |
1766276133539545088 |