Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean

• Published in: Applied and Environmental Microbiology
• Main Authors: Teira, E., Reinthaler, T., Pernthaler, Annelie, Pernthaler, J., Herndl, G.J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology (ASM), Washington, DC 2004
• Subjects: North Atlantic
• Online Access: https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575; https://doi.org/10.1128/AEM.70.7.4411-4414.2004

The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%).