Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean
The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with micro...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology (ASM), Washington, DC
2004
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Online Access: | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575 https://doi.org/10.1128/AEM.70.7.4411-4414.2004 |
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ftufz:oai:ufz.de:4575 2023-12-10T09:51:20+01:00 Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean Teira, E. Reinthaler, T. Pernthaler, Annelie Pernthaler, J. Herndl, G.J. 2004 application/pdf https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575 https://doi.org/10.1128/AEM.70.7.4411-4414.2004 en eng American Society for Microbiology (ASM), Washington, DC Applied and Environmental Microbiology 70 (7);; 4411 - 4414 https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575 https://dx.doi.org/10.1128/AEM.70.7.4411-4414.2004 info:eu-repo/semantics/openAccess ISSN: 0099-2240 info:eu-repo/semantics/article https://purl.org/dc/dcmitype/Text 2004 ftufz https://doi.org/10.1128/AEM.70.7.4411-4414.2004 2023-11-12T23:28:49Z The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%). Article in Journal/Newspaper North Atlantic UFZ - Publication Index (Helmholtz-Centre for Environmental Research) Applied and Environmental Microbiology 70 7 4411 4414 |
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UFZ - Publication Index (Helmholtz-Centre for Environmental Research) |
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ftufz |
language |
English |
description |
The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%). |
format |
Article in Journal/Newspaper |
author |
Teira, E. Reinthaler, T. Pernthaler, Annelie Pernthaler, J. Herndl, G.J. |
spellingShingle |
Teira, E. Reinthaler, T. Pernthaler, Annelie Pernthaler, J. Herndl, G.J. Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
author_facet |
Teira, E. Reinthaler, T. Pernthaler, Annelie Pernthaler, J. Herndl, G.J. |
author_sort |
Teira, E. |
title |
Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
title_short |
Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
title_full |
Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
title_fullStr |
Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
title_full_unstemmed |
Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
title_sort |
combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
publisher |
American Society for Microbiology (ASM), Washington, DC |
publishDate |
2004 |
url |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575 https://doi.org/10.1128/AEM.70.7.4411-4414.2004 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 0099-2240 |
op_relation |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575 https://dx.doi.org/10.1128/AEM.70.7.4411-4414.2004 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/AEM.70.7.4411-4414.2004 |
container_title |
Applied and Environmental Microbiology |
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70 |
container_issue |
7 |
container_start_page |
4411 |
op_container_end_page |
4414 |
_version_ |
1784896887813308416 |