Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean
Fluorescence in situ hybridization (FISH) in combination with polynucleotide probes revealed that the two major groups of planktonic Archaea (Crenarchaeota and Euryarchaeota) exhibit a different distribution pattern in the water column of the Pacific subtropical gyre and in the Antarctic Circumpolar...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology (ASM), Washington, DC
2005
|
Subjects: | |
Online Access: | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3442 https://doi.org/10.1128/AEM.71.5.2303-2309.2005 |
id |
ftufz:oai:ufz.de:3442 |
---|---|
record_format |
openpolar |
spelling |
ftufz:oai:ufz.de:3442 2023-12-10T09:41:58+01:00 Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean Herndl, G.J. Reinthaler, T. Teira, E. van Aken, H. Veth, C. Pernthaler, Annelie Pernthaler, J. 2005 application/pdf https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3442 https://doi.org/10.1128/AEM.71.5.2303-2309.2005 en eng American Society for Microbiology (ASM), Washington, DC Applied and Environmental Microbiology 71 (5);; 2303 - 2309 https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3442 https://dx.doi.org/10.1128/AEM.71.5.2303-2309.2005 info:eu-repo/semantics/openAccess ISSN: 0099-2240 info:eu-repo/semantics/article https://purl.org/dc/dcmitype/Text 2005 ftufz https://doi.org/10.1128/AEM.71.5.2303-2309.2005 2023-11-12T23:28:24Z Fluorescence in situ hybridization (FISH) in combination with polynucleotide probes revealed that the two major groups of planktonic Archaea (Crenarchaeota and Euryarchaeota) exhibit a different distribution pattern in the water column of the Pacific subtropical gyre and in the Antarctic Circumpolar Current system. While Euryarchaeota were found to be more dominant in nearsurface waters, Crenarchaeota were relatively more abundant in the mesopelagic and bathypelagic waters. We determined the abundance of archaea in the mesopelagic and bathypelagic North Atlantic along a south-north transect of more than 4,000 km. Using an improved catalyzed reporter deposition-FISH (CARD-FISH) method and specific oligonucleotide probes, we found that archaea were consistently more abundant than bacteria below a 100-m depth. Combining microautoradiography with CARD-FISH revealed a high fraction of metabolically active cells in the deep ocean. Even at a 3,000-m depth, about 16% of the bacteria were taking up leucine. The percentage of Euryarchaeota and Crenarchaeaota taking up leucine did not follow a specific trend, with depths ranging from 6 to 35% and 3 to 18%, respectively. The fraction of Crenarchaeota taking up inorganic carbon increased with depth, while Euryarchaeota taking up inorganic carbon decreased from 200 m to 3,000 m in depth. The ability of archaea to take up inorganic carbon was used as a proxy to estimate archaeal cell production and to compare this archaeal production with total prokaryotic production measured via leucine incorporation. We estimate that archaeal production in the mesopelagic and bathypelagic North Atlantic contributes between 13 to 27% to the total prokaryotic production in the oxygen minimum layer and 41 to 84% in the Labrador Sea Water, declining to 10 to 20% in the North Atlantic Deep Water. Thus, planktonic archaea are actively growing in the dark ocean although at lower growth rates than bacteria and might play a significant role in the oceanic carbon cycle. Article in Journal/Newspaper Antarc* Antarctic Labrador Sea North Atlantic Deep Water North Atlantic UFZ - Publication Index (Helmholtz-Centre for Environmental Research) Antarctic The Antarctic Pacific Applied and Environmental Microbiology 71 5 2303 2309 |
institution |
Open Polar |
collection |
UFZ - Publication Index (Helmholtz-Centre for Environmental Research) |
op_collection_id |
ftufz |
language |
English |
description |
Fluorescence in situ hybridization (FISH) in combination with polynucleotide probes revealed that the two major groups of planktonic Archaea (Crenarchaeota and Euryarchaeota) exhibit a different distribution pattern in the water column of the Pacific subtropical gyre and in the Antarctic Circumpolar Current system. While Euryarchaeota were found to be more dominant in nearsurface waters, Crenarchaeota were relatively more abundant in the mesopelagic and bathypelagic waters. We determined the abundance of archaea in the mesopelagic and bathypelagic North Atlantic along a south-north transect of more than 4,000 km. Using an improved catalyzed reporter deposition-FISH (CARD-FISH) method and specific oligonucleotide probes, we found that archaea were consistently more abundant than bacteria below a 100-m depth. Combining microautoradiography with CARD-FISH revealed a high fraction of metabolically active cells in the deep ocean. Even at a 3,000-m depth, about 16% of the bacteria were taking up leucine. The percentage of Euryarchaeota and Crenarchaeaota taking up leucine did not follow a specific trend, with depths ranging from 6 to 35% and 3 to 18%, respectively. The fraction of Crenarchaeota taking up inorganic carbon increased with depth, while Euryarchaeota taking up inorganic carbon decreased from 200 m to 3,000 m in depth. The ability of archaea to take up inorganic carbon was used as a proxy to estimate archaeal cell production and to compare this archaeal production with total prokaryotic production measured via leucine incorporation. We estimate that archaeal production in the mesopelagic and bathypelagic North Atlantic contributes between 13 to 27% to the total prokaryotic production in the oxygen minimum layer and 41 to 84% in the Labrador Sea Water, declining to 10 to 20% in the North Atlantic Deep Water. Thus, planktonic archaea are actively growing in the dark ocean although at lower growth rates than bacteria and might play a significant role in the oceanic carbon cycle. |
format |
Article in Journal/Newspaper |
author |
Herndl, G.J. Reinthaler, T. Teira, E. van Aken, H. Veth, C. Pernthaler, Annelie Pernthaler, J. |
spellingShingle |
Herndl, G.J. Reinthaler, T. Teira, E. van Aken, H. Veth, C. Pernthaler, Annelie Pernthaler, J. Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean |
author_facet |
Herndl, G.J. Reinthaler, T. Teira, E. van Aken, H. Veth, C. Pernthaler, Annelie Pernthaler, J. |
author_sort |
Herndl, G.J. |
title |
Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean |
title_short |
Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean |
title_full |
Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean |
title_fullStr |
Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean |
title_full_unstemmed |
Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean |
title_sort |
contribution of archaea to total prokaryotic production in the deep atlantic ocean |
publisher |
American Society for Microbiology (ASM), Washington, DC |
publishDate |
2005 |
url |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3442 https://doi.org/10.1128/AEM.71.5.2303-2309.2005 |
geographic |
Antarctic The Antarctic Pacific |
geographic_facet |
Antarctic The Antarctic Pacific |
genre |
Antarc* Antarctic Labrador Sea North Atlantic Deep Water North Atlantic |
genre_facet |
Antarc* Antarctic Labrador Sea North Atlantic Deep Water North Atlantic |
op_source |
ISSN: 0099-2240 |
op_relation |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3442 https://dx.doi.org/10.1128/AEM.71.5.2303-2309.2005 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/AEM.71.5.2303-2309.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
5 |
container_start_page |
2303 |
op_container_end_page |
2309 |
_version_ |
1784884690477383680 |