Degradation of crude oil by an arctic microbial consortium
The ability of a psychrotolerant microbial consortium to degrade crude oil at low temperatures was investigated. The enriched arctic microbial community was also tested for its ability to utilize various hydrocarbons, such as long-chain alkanes (n-C24 to n-C34), pristane, (methyl-)naphthalenes, and...
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ftufz:oai:ufz.de:3274 2023-12-10T09:45:25+01:00 Degradation of crude oil by an arctic microbial consortium Deppe, U. Richnow, Hans Hermann Michaelis, W. Antranikian, G. 2005 application/pdf https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3274 https://doi.org/10.1007/s00792-005-0463-2 en eng Springer Extremophiles 9 (6);; 461 - 470 https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3274 https://dx.doi.org/10.1007/s00792-005-0463-2 info:eu-repo/semantics/closedAccess ISSN: 1431-0651 info:eu-repo/semantics/article https://purl.org/dc/dcmitype/Text 2005 ftufz https://doi.org/10.1007/s00792-005-0463-2 2023-11-12T23:28:19Z The ability of a psychrotolerant microbial consortium to degrade crude oil at low temperatures was investigated. The enriched arctic microbial community was also tested for its ability to utilize various hydrocarbons, such as long-chain alkanes (n-C24 to n-C34), pristane, (methyl-)naphthalenes, and xylenes, as sole carbon and energy sources. Except for o-xylene and methylnaphthalenes, all tested compounds were metabolized under conditions that are typical for contaminated marine liquid sites, namely at pH 6–9 and at 4–27°C. By applying molecular biological techniques (16S rDNA sequencing, DGGE) nine strains could be identified in the consortium. Five of these strains could be isolated in pure cultures. The involved strains were closely related to the following genera: Pseudoalteromonas (two species), Pseudomonas (two species), Shewanella (two species), Marinobacter (one species), Psychrobacter (one species), and Agreia (one species). Interestingly, the five isolated strains in different combinations were unable to degrade crude oil or its components significantly, indicating the importance of the four unculturable microorganisms in the degradation of single or of complex mixtures of hydrocarbons. The obtained mixed culture showed obvious advantages including stability of the consortium, wide range adaptability for crude oil degradation, and strong degradation ability of crude oil. Article in Journal/Newspaper Arctic UFZ - Publication Index (Helmholtz-Centre for Environmental Research) Arctic Extremophiles 9 6 461 470 |
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Open Polar |
collection |
UFZ - Publication Index (Helmholtz-Centre for Environmental Research) |
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ftufz |
language |
English |
description |
The ability of a psychrotolerant microbial consortium to degrade crude oil at low temperatures was investigated. The enriched arctic microbial community was also tested for its ability to utilize various hydrocarbons, such as long-chain alkanes (n-C24 to n-C34), pristane, (methyl-)naphthalenes, and xylenes, as sole carbon and energy sources. Except for o-xylene and methylnaphthalenes, all tested compounds were metabolized under conditions that are typical for contaminated marine liquid sites, namely at pH 6–9 and at 4–27°C. By applying molecular biological techniques (16S rDNA sequencing, DGGE) nine strains could be identified in the consortium. Five of these strains could be isolated in pure cultures. The involved strains were closely related to the following genera: Pseudoalteromonas (two species), Pseudomonas (two species), Shewanella (two species), Marinobacter (one species), Psychrobacter (one species), and Agreia (one species). Interestingly, the five isolated strains in different combinations were unable to degrade crude oil or its components significantly, indicating the importance of the four unculturable microorganisms in the degradation of single or of complex mixtures of hydrocarbons. The obtained mixed culture showed obvious advantages including stability of the consortium, wide range adaptability for crude oil degradation, and strong degradation ability of crude oil. |
format |
Article in Journal/Newspaper |
author |
Deppe, U. Richnow, Hans Hermann Michaelis, W. Antranikian, G. |
spellingShingle |
Deppe, U. Richnow, Hans Hermann Michaelis, W. Antranikian, G. Degradation of crude oil by an arctic microbial consortium |
author_facet |
Deppe, U. Richnow, Hans Hermann Michaelis, W. Antranikian, G. |
author_sort |
Deppe, U. |
title |
Degradation of crude oil by an arctic microbial consortium |
title_short |
Degradation of crude oil by an arctic microbial consortium |
title_full |
Degradation of crude oil by an arctic microbial consortium |
title_fullStr |
Degradation of crude oil by an arctic microbial consortium |
title_full_unstemmed |
Degradation of crude oil by an arctic microbial consortium |
title_sort |
degradation of crude oil by an arctic microbial consortium |
publisher |
Springer |
publishDate |
2005 |
url |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3274 https://doi.org/10.1007/s00792-005-0463-2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1431-0651 |
op_relation |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3274 https://dx.doi.org/10.1007/s00792-005-0463-2 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00792-005-0463-2 |
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Extremophiles |
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9 |
container_issue |
6 |
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461 |
op_container_end_page |
470 |
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1784888680049016832 |