Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate
Following the ban of polybrominated diphenyl ether (PBDEs) flame retardants under well-documented toxicity issues, organophosphate such as tris(2-butoxyethyl) phosphate (TBOEP) and tris(2-cloroethyl) phosphate (TCEP) were considered as potential substitutes. Although TBOEP and TCEP are consistently...
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ftufz:oai:ufz.de:17521 2023-12-10T09:46:55+01:00 Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate Arukwe, A. Carteny, C.C. Möder, Monika Bonini, A. Maubach, M.A. Eggen, T. 2016-03-26 application/pdf https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17521 https://doi.org/10.1016/j.envres.2016.03.020 en eng Elsevier Environmental Research 148;; 63 - 71 https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17521 https://dx.doi.org/10.1016/j.envres.2016.03.020 info:eu-repo/semantics/closedAccess ISSN: 0013-9351 Fish TBOEP TCEP OPFRs Steroidogenesis Endocrine disruptor info:eu-repo/semantics/article https://purl.org/dc/dcmitype/Text 2016 ftufz https://doi.org/10.1016/j.envres.2016.03.020 2023-11-12T23:33:50Z Following the ban of polybrominated diphenyl ether (PBDEs) flame retardants under well-documented toxicity issues, organophosphate such as tris(2-butoxyethyl) phosphate (TBOEP) and tris(2-cloroethyl) phosphate (TCEP) were considered as potential substitutes. Although TBOEP and TCEP are consistently detected in the aquatic environment, there are few data about the possible toxicological effects of these compounds on aquatic organisms, including fish. In the present study, we have investigated the influence of TBOEP and TCEP on neuro- and interrenal steroidogenesis of juvenile Atlantic salmon (Salmo salar), after a seven-day exposure to four different concentrations (0 (control), 0.04, 0.2 and 1 mg/L) of each compound. TBOEP and TCEP were diluted in Milli-Q water. The expression of genes involved in steroidogenesis (StAR, cyp19a, cyp19b, cholesterol side-chain cleavage enzyme (P450scc), 3β-hydroxysteroid dehydrogenase (3β-hsd), and 11β-hydroxylase (cyp11β)), were analyzed in the brain and head kidney using real-time PCR. Plasma 11-ketotestosterone (11-KT) analysis was performed using enzyme immunoassay (EIA). Our results showed that TBOEP accumulated more rapidly than TCEP in fish muscle tissue. Surprisingly, TBOEP produced less pronounced effects than TCEP on neural and interrenal steroidogenic responses, despite the observed rapid uptake and bioaccumulation pattern. Specifically, TBOEP produced significant and consistent concentration-specific alterations on neural- and interrenal steroidogenesis. Plasma levels of 11-KT were not significantly altered by any of the exposures. The increased expression of steroidogenic genes demonstrated in the present study could produce time-specific alterations in the production of glucocorticoids and steroid hormones that play integral roles in fish metabolism, stress responses and adaptation, sexual maturation, reproduction and migration with overt consequences on reproductive success and survival. Article in Journal/Newspaper Atlantic salmon Salmo salar UFZ - Publication Index (Helmholtz-Centre for Environmental Research) Eia ENVELOPE(7.755,7.755,63.024,63.024) Environmental Research 148 63 71 |
institution |
Open Polar |
collection |
UFZ - Publication Index (Helmholtz-Centre for Environmental Research) |
op_collection_id |
ftufz |
language |
English |
topic |
Fish TBOEP TCEP OPFRs Steroidogenesis Endocrine disruptor |
spellingShingle |
Fish TBOEP TCEP OPFRs Steroidogenesis Endocrine disruptor Arukwe, A. Carteny, C.C. Möder, Monika Bonini, A. Maubach, M.A. Eggen, T. Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
topic_facet |
Fish TBOEP TCEP OPFRs Steroidogenesis Endocrine disruptor |
description |
Following the ban of polybrominated diphenyl ether (PBDEs) flame retardants under well-documented toxicity issues, organophosphate such as tris(2-butoxyethyl) phosphate (TBOEP) and tris(2-cloroethyl) phosphate (TCEP) were considered as potential substitutes. Although TBOEP and TCEP are consistently detected in the aquatic environment, there are few data about the possible toxicological effects of these compounds on aquatic organisms, including fish. In the present study, we have investigated the influence of TBOEP and TCEP on neuro- and interrenal steroidogenesis of juvenile Atlantic salmon (Salmo salar), after a seven-day exposure to four different concentrations (0 (control), 0.04, 0.2 and 1 mg/L) of each compound. TBOEP and TCEP were diluted in Milli-Q water. The expression of genes involved in steroidogenesis (StAR, cyp19a, cyp19b, cholesterol side-chain cleavage enzyme (P450scc), 3β-hydroxysteroid dehydrogenase (3β-hsd), and 11β-hydroxylase (cyp11β)), were analyzed in the brain and head kidney using real-time PCR. Plasma 11-ketotestosterone (11-KT) analysis was performed using enzyme immunoassay (EIA). Our results showed that TBOEP accumulated more rapidly than TCEP in fish muscle tissue. Surprisingly, TBOEP produced less pronounced effects than TCEP on neural and interrenal steroidogenic responses, despite the observed rapid uptake and bioaccumulation pattern. Specifically, TBOEP produced significant and consistent concentration-specific alterations on neural- and interrenal steroidogenesis. Plasma levels of 11-KT were not significantly altered by any of the exposures. The increased expression of steroidogenic genes demonstrated in the present study could produce time-specific alterations in the production of glucocorticoids and steroid hormones that play integral roles in fish metabolism, stress responses and adaptation, sexual maturation, reproduction and migration with overt consequences on reproductive success and survival. |
format |
Article in Journal/Newspaper |
author |
Arukwe, A. Carteny, C.C. Möder, Monika Bonini, A. Maubach, M.A. Eggen, T. |
author_facet |
Arukwe, A. Carteny, C.C. Möder, Monika Bonini, A. Maubach, M.A. Eggen, T. |
author_sort |
Arukwe, A. |
title |
Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
title_short |
Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
title_full |
Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
title_fullStr |
Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
title_full_unstemmed |
Differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
title_sort |
differential modulation of neuro- and interrenal steroidogenesis of juvenile salmon by the organophosphates - tris(2-butoxyethyl)- and tris(2-cloroethyl) phosphate |
publisher |
Elsevier |
publishDate |
2016 |
url |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17521 https://doi.org/10.1016/j.envres.2016.03.020 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0013-9351 |
op_relation |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17521 https://dx.doi.org/10.1016/j.envres.2016.03.020 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.envres.2016.03.020 |
container_title |
Environmental Research |
container_volume |
148 |
container_start_page |
63 |
op_container_end_page |
71 |
_version_ |
1784890474766532608 |