Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean
In flow cytometric analyses of marine prokaryotic picoplankton often two populations with distinct differences in their apparent nucleic acid content are discernable, one with a high and one with a low nucleic acid content (HNA and LNA, respectively). In this study we determined the phylogenetic com...
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ftufz:oai:ufz.de:11057 2023-12-10T09:45:15+01:00 Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean Schattenhofer, Martha Wulf, J. Kostadinov, I. Glöckner, F.O. Zubkov, M.V. Fuchs, B.M. 2011 application/pdf https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11057 https://doi.org/10.1016/j.syapm.2011.01.008 en eng Elsevier Systematic and Applied Microbiology 34 (6);; 470 - 475 https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11057 https://dx.doi.org/10.1016/j.syapm.2011.01.008 info:eu-repo/semantics/closedAccess ISSN: 0723-2020 Flow cytometry CARD-FISH Prokaryotic picoplankton Marine Phylogenetic composition Genome size info:eu-repo/semantics/article https://purl.org/dc/dcmitype/Text 2011 ftufz https://doi.org/10.1016/j.syapm.2011.01.008 2023-11-12T23:31:21Z In flow cytometric analyses of marine prokaryotic picoplankton often two populations with distinct differences in their apparent nucleic acid content are discernable, one with a high and one with a low nucleic acid content (HNA and LNA, respectively). In this study we determined the phylogenetic composition of flow cytometrically sorted HNA and LNA populations, collected at six stations along a transect across three oceanic provinces from Iceland to the Azores. Catalysed reporter deposition fluorescence in situ hybridisation (CARD-FISH) analysis of sorted cells revealed distinct differences in phylogenetic composition between the LNA and HNA populations with only little overlap. At all stations the LNA population was dominated by the alphaproteobacterial clade SAR11 (45-74%). Also, Betaproteobacteria were always present at 2-4%. While the LNA composition was rather stable, the HNA populations were composed of distinct phylogenetic clades in the different oceanic provinces of Arctic and Tropics. For example Cyanobacteria dominated the North Atlantic Gyre HNA population (29-44%) with Prochlorococcus as the major clade (34-44%), but were low in Arctic and Polar waters (1% and 5%, respectively). In contrast, Bacteroidetes accounted for the majority of HNA cells in the Polar and Arctic province (26% and 32%, respectively), but were low in the Gyre region (3-10%). The DNA content of the HNA population was about 3.5 times higher than that of the LNA populations. This reflects differences in the genome sizes of closely related cultured representatives of HNA clades (3-6 Mbp) and LNA clades (1.3-1.5 Mbp). Article in Journal/Newspaper Arctic Iceland North Atlantic UFZ - Publication Index (Helmholtz-Centre for Environmental Research) Arctic Systematic and Applied Microbiology 34 6 470 475 |
institution |
Open Polar |
collection |
UFZ - Publication Index (Helmholtz-Centre for Environmental Research) |
op_collection_id |
ftufz |
language |
English |
topic |
Flow cytometry CARD-FISH Prokaryotic picoplankton Marine Phylogenetic composition Genome size |
spellingShingle |
Flow cytometry CARD-FISH Prokaryotic picoplankton Marine Phylogenetic composition Genome size Schattenhofer, Martha Wulf, J. Kostadinov, I. Glöckner, F.O. Zubkov, M.V. Fuchs, B.M. Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean |
topic_facet |
Flow cytometry CARD-FISH Prokaryotic picoplankton Marine Phylogenetic composition Genome size |
description |
In flow cytometric analyses of marine prokaryotic picoplankton often two populations with distinct differences in their apparent nucleic acid content are discernable, one with a high and one with a low nucleic acid content (HNA and LNA, respectively). In this study we determined the phylogenetic composition of flow cytometrically sorted HNA and LNA populations, collected at six stations along a transect across three oceanic provinces from Iceland to the Azores. Catalysed reporter deposition fluorescence in situ hybridisation (CARD-FISH) analysis of sorted cells revealed distinct differences in phylogenetic composition between the LNA and HNA populations with only little overlap. At all stations the LNA population was dominated by the alphaproteobacterial clade SAR11 (45-74%). Also, Betaproteobacteria were always present at 2-4%. While the LNA composition was rather stable, the HNA populations were composed of distinct phylogenetic clades in the different oceanic provinces of Arctic and Tropics. For example Cyanobacteria dominated the North Atlantic Gyre HNA population (29-44%) with Prochlorococcus as the major clade (34-44%), but were low in Arctic and Polar waters (1% and 5%, respectively). In contrast, Bacteroidetes accounted for the majority of HNA cells in the Polar and Arctic province (26% and 32%, respectively), but were low in the Gyre region (3-10%). The DNA content of the HNA population was about 3.5 times higher than that of the LNA populations. This reflects differences in the genome sizes of closely related cultured representatives of HNA clades (3-6 Mbp) and LNA clades (1.3-1.5 Mbp). |
format |
Article in Journal/Newspaper |
author |
Schattenhofer, Martha Wulf, J. Kostadinov, I. Glöckner, F.O. Zubkov, M.V. Fuchs, B.M. |
author_facet |
Schattenhofer, Martha Wulf, J. Kostadinov, I. Glöckner, F.O. Zubkov, M.V. Fuchs, B.M. |
author_sort |
Schattenhofer, Martha |
title |
Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean |
title_short |
Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean |
title_full |
Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean |
title_fullStr |
Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean |
title_full_unstemmed |
Phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the North Atlantic Ocean |
title_sort |
phylogenetic characterisation of picoplanktonic populations with high and low nucleic acid content in the north atlantic ocean |
publisher |
Elsevier |
publishDate |
2011 |
url |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11057 https://doi.org/10.1016/j.syapm.2011.01.008 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Iceland North Atlantic |
genre_facet |
Arctic Iceland North Atlantic |
op_source |
ISSN: 0723-2020 |
op_relation |
https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11057 https://dx.doi.org/10.1016/j.syapm.2011.01.008 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.syapm.2011.01.008 |
container_title |
Systematic and Applied Microbiology |
container_volume |
34 |
container_issue |
6 |
container_start_page |
470 |
op_container_end_page |
475 |
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1784888488255029248 |