Collagen-based bioactive bromelain hydrolysate from salt-cured cod skin

• Published in: Applied Sciences
• Main Authors: Coscueta, Ezequiel R., Brassesco, Maria Emilia, Pintado, Manuela
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2021
• Subjects: Antihypertensive; Antioxidant; Atlantic cod skin; Bioactive peptides; Bromelain; Collagen; Enzymatic hydrolysis; Fishery by-products; atlantic cod
• Online Access: https://ciencia.ucp.pt/en/publications/7f69752d-c4ff-451c-bf28-01bb396a9442; https://doi.org/10.3390/app11188538; https://ciencia.ucp.pt/ws/files/38199648/38199538.pdf; http://www.scopus.com/inward/record.url?scp=85115099262&partnerID=8YFLogxK; http://hdl.handle.net/10400.14/34996

Considerable amounts of fish processing by-products are discarded each year. About 30% of this material may be skin and bone. Fish skin has more than 80% of its total protein content as collagen. Furthermore, in recent years, there has been a growing demand for collagen-based peptides due to their beneficial health effects. So, the objective of the present study was to optimise the obtaining bioactive hydrolysates from salt-cured cod skin using the protease Bromelain at 0.5% (w/w) concentration. This study developed a sustainable process that consumes less time and energy and uses an alternative source as raw material. In addition, bromelain allows hydrolysates with important antioxidant (ORAC, 514 μmol Trolox Equivalent/g protein) and antihypertensive activities (inhibition of ACE, IC50 of 166 μg protein/mL) as well as excellent biocompatibility with dermal and subcutaneous cells.