Collagen-based bioactive bromelain hydrolysate from salt-cured cod skin
Considerable amounts of fish processing by-products are discarded each year. About 30% of this material may be skin and bone. Fish skin has more than 80% of its total protein content as collagen. Furthermore, in recent years, there has been a growing demand for collagen-based peptides due to their b...
Published in: | Applied Sciences |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2021
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Subjects: | |
Online Access: | https://ciencia.ucp.pt/en/publications/7f69752d-c4ff-451c-bf28-01bb396a9442 https://doi.org/10.3390/app11188538 https://ciencia.ucp.pt/ws/files/38199648/38199538.pdf http://www.scopus.com/inward/record.url?scp=85115099262&partnerID=8YFLogxK http://hdl.handle.net/10400.14/34996 |
Summary: | Considerable amounts of fish processing by-products are discarded each year. About 30% of this material may be skin and bone. Fish skin has more than 80% of its total protein content as collagen. Furthermore, in recent years, there has been a growing demand for collagen-based peptides due to their beneficial health effects. So, the objective of the present study was to optimise the obtaining bioactive hydrolysates from salt-cured cod skin using the protease Bromelain at 0.5% (w/w) concentration. This study developed a sustainable process that consumes less time and energy and uses an alternative source as raw material. In addition, bromelain allows hydrolysates with important antioxidant (ORAC, 514 μmol Trolox Equivalent/g protein) and antihypertensive activities (inhibition of ACE, IC50 of 166 μg protein/mL) as well as excellent biocompatibility with dermal and subcutaneous cells. |
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