Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica
Introduction. Pineapple is the second most important crop in Costa Rica, after banana. The Golden MD-2 variety standing out as the one with the highest demand in the United States and Europe. In 2019, the cultivation area was 40 000 ha distributed in the North, Atlantic and Pacific zones. Objective....
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Universidad de Costa Rica
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ftucostaricaojs:oai:portal.ucr.ac.cr:article/49867 2023-07-30T04:05:40+02:00 Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica Estudio preliminar de especies de Fusarium presentes en piña (Ananas comosus) en Costa Rica Blanco Meneses, Mónica Castro Zúñiga, Oscar Umaña Rojas, Gerardina 2022-01-26 application/pdf application/epub+zip text/html https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867 https://doi.org/10.15517/rac.v46i1.49867 spa spa Universidad de Costa Rica https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867/49878 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867/50121 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867/50296 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867 doi:10.15517/rac.v46i1.49867 Derechos de autor 2022 Agronomía Costarricense Agronomía Costarricense; Agronomía Costarricense: Vol. 46, Issue 1 (January - June) Agronomía Costarricense; Agronomía Costarricense: Vol. 46, Nº 1 (Enero - Junio) 2215-2202 0377-9424 10.15517/rac.v46i1 Síntomas Morfología Identificación molecular Symptoms Morphology Molecular identification info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article 2022 ftucostaricaojs https://doi.org/10.15517/rac.v46i1.4986710.15517/rac.v46i1 2023-07-11T21:04:31Z Introduction. Pineapple is the second most important crop in Costa Rica, after banana. The Golden MD-2 variety standing out as the one with the highest demand in the United States and Europe. In 2019, the cultivation area was 40 000 ha distributed in the North, Atlantic and Pacific zones. Objective. To determine the presence of the pathogen Fusarium guttiforme, and to identify and characterize the present species of this genus, in the pineapple crop in Costa Rica. Materials and methods. 215 plants and 20 fruits were collected from different regions between the 2015-2019 in a total of 38 175 ha of the pineapple planted area through a sampling directed towards symptoms related to the “descending death of pineapple”. For the morphological characterization observations were made of the color of the mycelium, details of structures such as macro and microconidia, chlamydospores and conidiogenic cells in samples previously identified by molecular means. Molecular identification was carried out from 120 isolates with the molecular markers ITS and TEF-1α. Results. F. guttiforme was not detected in pineapple plantations in the period between 2015-2019. Six species were identified, Fusarium ananatum, F. oxysporum, F. concolor, F. proliferatum, F. incarnatum and F. solani whose accession numbers in the National Center of Biotechnology Information are attached in this article. The species F. ananatum, F. oxysporum and F. proliferatum have been previously described as pathogenic in pineapple. The presence of F. ananatum in 95% of the collected fruit was related with the disease “fruitlet core rot”. F. proliferatum is a generator of mycotoxins, which could represent a danger to animal and human health. The other species have not been reported in pineapple before. Conclusion. The presence of the pathogen F. guttiforme was not found, although there were identified the species of this genus, present in pineapple cultivation area in Costa Rica. Introducción. La piña es el segundo cultivo de mayor importancia en Costa Rica, luego ... Article in Journal/Newspaper North Atlantic Portal de revistas académicas de la Universidad de Costa Rica Pacific Agronomía Costarricense |
institution |
Open Polar |
collection |
Portal de revistas académicas de la Universidad de Costa Rica |
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ftucostaricaojs |
language |
Spanish |
topic |
Síntomas Morfología Identificación molecular Symptoms Morphology Molecular identification |
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Síntomas Morfología Identificación molecular Symptoms Morphology Molecular identification Blanco Meneses, Mónica Castro Zúñiga, Oscar Umaña Rojas, Gerardina Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica |
topic_facet |
Síntomas Morfología Identificación molecular Symptoms Morphology Molecular identification |
description |
Introduction. Pineapple is the second most important crop in Costa Rica, after banana. The Golden MD-2 variety standing out as the one with the highest demand in the United States and Europe. In 2019, the cultivation area was 40 000 ha distributed in the North, Atlantic and Pacific zones. Objective. To determine the presence of the pathogen Fusarium guttiforme, and to identify and characterize the present species of this genus, in the pineapple crop in Costa Rica. Materials and methods. 215 plants and 20 fruits were collected from different regions between the 2015-2019 in a total of 38 175 ha of the pineapple planted area through a sampling directed towards symptoms related to the “descending death of pineapple”. For the morphological characterization observations were made of the color of the mycelium, details of structures such as macro and microconidia, chlamydospores and conidiogenic cells in samples previously identified by molecular means. Molecular identification was carried out from 120 isolates with the molecular markers ITS and TEF-1α. Results. F. guttiforme was not detected in pineapple plantations in the period between 2015-2019. Six species were identified, Fusarium ananatum, F. oxysporum, F. concolor, F. proliferatum, F. incarnatum and F. solani whose accession numbers in the National Center of Biotechnology Information are attached in this article. The species F. ananatum, F. oxysporum and F. proliferatum have been previously described as pathogenic in pineapple. The presence of F. ananatum in 95% of the collected fruit was related with the disease “fruitlet core rot”. F. proliferatum is a generator of mycotoxins, which could represent a danger to animal and human health. The other species have not been reported in pineapple before. Conclusion. The presence of the pathogen F. guttiforme was not found, although there were identified the species of this genus, present in pineapple cultivation area in Costa Rica. Introducción. La piña es el segundo cultivo de mayor importancia en Costa Rica, luego ... |
format |
Article in Journal/Newspaper |
author |
Blanco Meneses, Mónica Castro Zúñiga, Oscar Umaña Rojas, Gerardina |
author_facet |
Blanco Meneses, Mónica Castro Zúñiga, Oscar Umaña Rojas, Gerardina |
author_sort |
Blanco Meneses, Mónica |
title |
Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica |
title_short |
Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica |
title_full |
Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica |
title_fullStr |
Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica |
title_full_unstemmed |
Preliminary study of Fusarium species in pineapple crop (Ananas comosus) in Costa Rica |
title_sort |
preliminary study of fusarium species in pineapple crop (ananas comosus) in costa rica |
publisher |
Universidad de Costa Rica |
publishDate |
2022 |
url |
https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867 https://doi.org/10.15517/rac.v46i1.49867 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Agronomía Costarricense; Agronomía Costarricense: Vol. 46, Issue 1 (January - June) Agronomía Costarricense; Agronomía Costarricense: Vol. 46, Nº 1 (Enero - Junio) 2215-2202 0377-9424 10.15517/rac.v46i1 |
op_relation |
https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867/49878 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867/50121 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867/50296 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/49867 doi:10.15517/rac.v46i1.49867 |
op_rights |
Derechos de autor 2022 Agronomía Costarricense |
op_doi |
https://doi.org/10.15517/rac.v46i1.4986710.15517/rac.v46i1 |
container_title |
Agronomía Costarricense |
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