Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach.
In the Antarctic marine environment, the water temperature is usually between 2 and - 1.9 °C. Consequently, some Antarctic species have lost the capacity to adapt to sudden changes in temperature. The study of the immune response in Antarctic sea urchin (Sterechinus neumayeri) could help us understa...
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Universidad de Costa Rica
2016
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ftucostaricaojs:oai:portal.ucr.ac.cr:article/23165 2023-07-30T03:58:16+02:00 Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. Respuesta inmune del erizo de mar Antártico Sterechinus neumayeri: un enfoque celular, molecular y fisiológico. Gonzalez-Aravena, Marcelo Perez-Troncoso, Carolina Urtubia, Rocio Branco, Paola Machado Cunha da Silva, José Roberto Mercado, Luis De Lorgeril, Julien Bethke, Jorn Paschke, Kurt 2016-02-04 application/pdf text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165 https://doi.org/10.15517/rbt.v63i2.23165 eng eng Universidad de Costa Rica https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165/23472 https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165/23473 https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165 doi:10.15517/rbt.v63i2.23165 Revista de Biología Tropical; Vol. 63 No. S2 (2015): Volume 63 – Supplement 2 – June 2015: Research on equinoderms in Latin America III; 309-320 Revista de Biología Tropical; Vol. 63 Núm. S2 (2015): Volumen 63 – Suplemento 2 – Junio 2015: Estudios latinoamericanos en equinodermos III; 309-320 Revista Biología Tropical; Vol. 63 N.º S2 (2015): Volumen 63 – Suplemento 2 – Junio 2015: Estudios latinoamericanos en equinodermos III; 309-320 2215-2075 0034-7744 10.15517/rbt.v63i2 Erizo de mar Antártida Sterechinus neumayeri coelomocytes fagocitosis expresión genética Sea urchin Antarctica phagocytosis gene expression info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article 2016 ftucostaricaojs https://doi.org/10.15517/rbt.v63i2.2316510.15517/rbt.v63i2 2023-07-11T20:42:47Z In the Antarctic marine environment, the water temperature is usually between 2 and - 1.9 °C. Consequently, some Antarctic species have lost the capacity to adapt to sudden changes in temperature. The study of the immune response in Antarctic sea urchin (Sterechinus neumayeri) could help us understand the future impacts of global warming on endemic animals in the Antarctic Peninsula. In this study, the Antarctic sea urchins were challenged with lipopolysaccharides and Vibrio alginolitycus. The cellular response was evaluated by the number of coelomocytes and phagocytosis. A significant increase was observed in red sphere cells and total coelomocytes in animals exposed to LPS. A significant rise in phagocytosis in animals stimulated by LPS was also evidenced. Moreover, the gene expression of three immune related genes was measured by qPCR, showing a rapid increase in their expression levels. By contrast, these immune genes showed a depression in their expression by a thermal effect at 5 and 10 °C. In addition, during bacterial injection, the oxygen consumption was higher in challenged animals. Our results showed that the immune response in the Antarctic sea urchin may be affected by acute thermal stress and that this immune response has a metabolic cost. En el medio ambiente de la Antártica la temperatura del agua es de entre 2 y - 1.9 °C. Por consecuencia ciertas especies han perdido la capacidad de adaptarse a los cambios repentinos de la temperatura del agua. El estudio de la respuesta inmune del erizo antártico (Sterechinus neumayeri) podría ayudar a comprender los futuros impactos en los animales endémicos del cambio climático en la Península Antártica. En este estudio nosotros hemos evaluado la respuesta inmunitaria de S. neumayeri respecto de estimulaciones con bacterias (Lipopolisacáridos y Vibrio alginolitycus) asi como durante el estrés térmico a 5 y 10 °C. La respuesta del erizo fue evaluada en relación al número de celomocitos circulantes, capacidad fagocítica de estos y por el análisis de la ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Antártica Antártida Portal de revistas académicas de la Universidad de Costa Rica Antarctic Antarctic Peninsula Consecuencia ENVELOPE(-60.783,-60.783,-63.717,-63.717) Erizo ENVELOPE(-64.250,-64.250,-65.317,-65.317) The Antarctic |
institution |
Open Polar |
collection |
Portal de revistas académicas de la Universidad de Costa Rica |
op_collection_id |
ftucostaricaojs |
language |
English |
topic |
Erizo de mar Antártida Sterechinus neumayeri coelomocytes fagocitosis expresión genética Sea urchin Antarctica phagocytosis gene expression |
spellingShingle |
Erizo de mar Antártida Sterechinus neumayeri coelomocytes fagocitosis expresión genética Sea urchin Antarctica phagocytosis gene expression Gonzalez-Aravena, Marcelo Perez-Troncoso, Carolina Urtubia, Rocio Branco, Paola Machado Cunha da Silva, José Roberto Mercado, Luis De Lorgeril, Julien Bethke, Jorn Paschke, Kurt Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. |
topic_facet |
Erizo de mar Antártida Sterechinus neumayeri coelomocytes fagocitosis expresión genética Sea urchin Antarctica phagocytosis gene expression |
description |
In the Antarctic marine environment, the water temperature is usually between 2 and - 1.9 °C. Consequently, some Antarctic species have lost the capacity to adapt to sudden changes in temperature. The study of the immune response in Antarctic sea urchin (Sterechinus neumayeri) could help us understand the future impacts of global warming on endemic animals in the Antarctic Peninsula. In this study, the Antarctic sea urchins were challenged with lipopolysaccharides and Vibrio alginolitycus. The cellular response was evaluated by the number of coelomocytes and phagocytosis. A significant increase was observed in red sphere cells and total coelomocytes in animals exposed to LPS. A significant rise in phagocytosis in animals stimulated by LPS was also evidenced. Moreover, the gene expression of three immune related genes was measured by qPCR, showing a rapid increase in their expression levels. By contrast, these immune genes showed a depression in their expression by a thermal effect at 5 and 10 °C. In addition, during bacterial injection, the oxygen consumption was higher in challenged animals. Our results showed that the immune response in the Antarctic sea urchin may be affected by acute thermal stress and that this immune response has a metabolic cost. En el medio ambiente de la Antártica la temperatura del agua es de entre 2 y - 1.9 °C. Por consecuencia ciertas especies han perdido la capacidad de adaptarse a los cambios repentinos de la temperatura del agua. El estudio de la respuesta inmune del erizo antártico (Sterechinus neumayeri) podría ayudar a comprender los futuros impactos en los animales endémicos del cambio climático en la Península Antártica. En este estudio nosotros hemos evaluado la respuesta inmunitaria de S. neumayeri respecto de estimulaciones con bacterias (Lipopolisacáridos y Vibrio alginolitycus) asi como durante el estrés térmico a 5 y 10 °C. La respuesta del erizo fue evaluada en relación al número de celomocitos circulantes, capacidad fagocítica de estos y por el análisis de la ... |
format |
Article in Journal/Newspaper |
author |
Gonzalez-Aravena, Marcelo Perez-Troncoso, Carolina Urtubia, Rocio Branco, Paola Machado Cunha da Silva, José Roberto Mercado, Luis De Lorgeril, Julien Bethke, Jorn Paschke, Kurt |
author_facet |
Gonzalez-Aravena, Marcelo Perez-Troncoso, Carolina Urtubia, Rocio Branco, Paola Machado Cunha da Silva, José Roberto Mercado, Luis De Lorgeril, Julien Bethke, Jorn Paschke, Kurt |
author_sort |
Gonzalez-Aravena, Marcelo |
title |
Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. |
title_short |
Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. |
title_full |
Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. |
title_fullStr |
Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. |
title_full_unstemmed |
Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach. |
title_sort |
immune response of the antarctic sea urchin sterechinus neumayeri: cellular, molecular and physiological approach. |
publisher |
Universidad de Costa Rica |
publishDate |
2016 |
url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165 https://doi.org/10.15517/rbt.v63i2.23165 |
long_lat |
ENVELOPE(-60.783,-60.783,-63.717,-63.717) ENVELOPE(-64.250,-64.250,-65.317,-65.317) |
geographic |
Antarctic Antarctic Peninsula Consecuencia Erizo The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Consecuencia Erizo The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Antártica Antártida |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Antártica Antártida |
op_source |
Revista de Biología Tropical; Vol. 63 No. S2 (2015): Volume 63 – Supplement 2 – June 2015: Research on equinoderms in Latin America III; 309-320 Revista de Biología Tropical; Vol. 63 Núm. S2 (2015): Volumen 63 – Suplemento 2 – Junio 2015: Estudios latinoamericanos en equinodermos III; 309-320 Revista Biología Tropical; Vol. 63 N.º S2 (2015): Volumen 63 – Suplemento 2 – Junio 2015: Estudios latinoamericanos en equinodermos III; 309-320 2215-2075 0034-7744 10.15517/rbt.v63i2 |
op_relation |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165/23472 https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165/23473 https://revistas.ucr.ac.cr/index.php/rbt/article/view/23165 doi:10.15517/rbt.v63i2.23165 |
op_doi |
https://doi.org/10.15517/rbt.v63i2.2316510.15517/rbt.v63i2 |
_version_ |
1772821116768747520 |