Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos
The effect of different temperatures (4 degreesC and 12 degreesC) on myogenic regulatory factors (MyoD and myogenin) and myosin heavy chain (MyHC) expression was investigated in rainbow trout (Onchrhynchus mykiss) during early development. MyoD is first switched on at stage 14 [about 5 somites are f...
Main Authors: | , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
BLACKWELL VERLAG GMBH
2001
|
Subjects: | |
Online Access: | http://discovery.ucl.ac.uk/27707/ |
id |
ftucl:oai:eprints.ucl.ac.uk.OAI2:27707 |
---|---|
record_format |
openpolar |
spelling |
ftucl:oai:eprints.ucl.ac.uk.OAI2:27707 2023-05-15T18:09:57+02:00 Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos Xie, SQ Mason, PS Wilkes, D Goldspink, G Fauconneau, B Stickland, NC 2001-09 http://discovery.ucl.ac.uk/27707/ unknown BLACKWELL VERLAG GMBH DIFFERENTIATION , 68 (2-3) 106 - 114. (2001) rainbow trout myogenin myosin development differentiation MYOSIN HEAVY-CHAIN HERRING CLUPEA-HARENGUS DICENTRARCHUS-LABRAX L SALMO-SALAR L SKELETAL-MUSCLE LATERAL MUSCLE MYOTOMAL MUSCULATURE ONCORHYNCHUS-MYKISS MOUSE EMBRYOGENESIS HYPERTROPHIC GROWTH Article 2001 ftucl 2013-11-09T21:41:44Z The effect of different temperatures (4 degreesC and 12 degreesC) on myogenic regulatory factors (MyoD and myogenin) and myosin heavy chain (MyHC) expression was investigated in rainbow trout (Onchrhynchus mykiss) during early development. MyoD is first switched on at stage 14 [about 5 somites are formed (1/2 epiboly)] while myogenin mRNA is expressed at stage 15 [around 15 somites are visible (2/3 epiboly)] at both temperatures. Subsequently (up to at least stage 20), the most caudal somites exhibit less myogenin mRNA at 4 degreesC compared to 12 degreesC. At the eyed stage (stage 23 - 24), both myogenin mRNA and protein are present in greater amounts throughout all myotomes at the lower temperature, with mRNA levels in warmer (12'C) embryos at 83% for MyoD and 72% for myogenin of the levels seen in 4 degreesC embryos. Conversely, however, at this same stage, fast-MyHC mRNA and protein are more abundant in 12'C than in 4 degreesC embryos. This indicates relatively advanced muscle differentiation at the warmer temperature. At hatching, myogenin-positive cells are concentrated within the myosepta at both temperatures and they are also sparsely distributed in the myotome at 4 degreesC, but not at 12'C. MyoD, myogenin, and MyHC levels provide an indication of differentiation of muscle cells. These findings suggest that myogenic regulatory factor expression is delayed but prolonged by the lowering of temperature. Article in Journal/Newspaper Salmo salar University College London: UCL Discovery |
institution |
Open Polar |
collection |
University College London: UCL Discovery |
op_collection_id |
ftucl |
language |
unknown |
topic |
rainbow trout myogenin myosin development differentiation MYOSIN HEAVY-CHAIN HERRING CLUPEA-HARENGUS DICENTRARCHUS-LABRAX L SALMO-SALAR L SKELETAL-MUSCLE LATERAL MUSCLE MYOTOMAL MUSCULATURE ONCORHYNCHUS-MYKISS MOUSE EMBRYOGENESIS HYPERTROPHIC GROWTH |
spellingShingle |
rainbow trout myogenin myosin development differentiation MYOSIN HEAVY-CHAIN HERRING CLUPEA-HARENGUS DICENTRARCHUS-LABRAX L SALMO-SALAR L SKELETAL-MUSCLE LATERAL MUSCLE MYOTOMAL MUSCULATURE ONCORHYNCHUS-MYKISS MOUSE EMBRYOGENESIS HYPERTROPHIC GROWTH Xie, SQ Mason, PS Wilkes, D Goldspink, G Fauconneau, B Stickland, NC Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos |
topic_facet |
rainbow trout myogenin myosin development differentiation MYOSIN HEAVY-CHAIN HERRING CLUPEA-HARENGUS DICENTRARCHUS-LABRAX L SALMO-SALAR L SKELETAL-MUSCLE LATERAL MUSCLE MYOTOMAL MUSCULATURE ONCORHYNCHUS-MYKISS MOUSE EMBRYOGENESIS HYPERTROPHIC GROWTH |
description |
The effect of different temperatures (4 degreesC and 12 degreesC) on myogenic regulatory factors (MyoD and myogenin) and myosin heavy chain (MyHC) expression was investigated in rainbow trout (Onchrhynchus mykiss) during early development. MyoD is first switched on at stage 14 [about 5 somites are formed (1/2 epiboly)] while myogenin mRNA is expressed at stage 15 [around 15 somites are visible (2/3 epiboly)] at both temperatures. Subsequently (up to at least stage 20), the most caudal somites exhibit less myogenin mRNA at 4 degreesC compared to 12 degreesC. At the eyed stage (stage 23 - 24), both myogenin mRNA and protein are present in greater amounts throughout all myotomes at the lower temperature, with mRNA levels in warmer (12'C) embryos at 83% for MyoD and 72% for myogenin of the levels seen in 4 degreesC embryos. Conversely, however, at this same stage, fast-MyHC mRNA and protein are more abundant in 12'C than in 4 degreesC embryos. This indicates relatively advanced muscle differentiation at the warmer temperature. At hatching, myogenin-positive cells are concentrated within the myosepta at both temperatures and they are also sparsely distributed in the myotome at 4 degreesC, but not at 12'C. MyoD, myogenin, and MyHC levels provide an indication of differentiation of muscle cells. These findings suggest that myogenic regulatory factor expression is delayed but prolonged by the lowering of temperature. |
format |
Article in Journal/Newspaper |
author |
Xie, SQ Mason, PS Wilkes, D Goldspink, G Fauconneau, B Stickland, NC |
author_facet |
Xie, SQ Mason, PS Wilkes, D Goldspink, G Fauconneau, B Stickland, NC |
author_sort |
Xie, SQ |
title |
Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos |
title_short |
Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos |
title_full |
Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos |
title_fullStr |
Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos |
title_full_unstemmed |
Lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (Onchrhynchus mykiss) embryos |
title_sort |
lower environmental temperature delays and prolongs myogenic regulatory factor expression and muscle differentiation in rainbow trout (onchrhynchus mykiss) embryos |
publisher |
BLACKWELL VERLAG GMBH |
publishDate |
2001 |
url |
http://discovery.ucl.ac.uk/27707/ |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
DIFFERENTIATION , 68 (2-3) 106 - 114. (2001) |
_version_ |
1766182655126142976 |