Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey
BACKGROUND: Population-based research on vitamin D has increased dramatically in recent years. Such studies are typically reliant on assay procedures to measure reliable and comparable levels of 25-hydroxyvitamin D [25(OH)D] concentrations. METHODS: Concentrations of 25(OH)D3 and 25(OH)D2 were measu...
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ftucl:oai:eprints.ucl.ac.uk.OAI2:1514947 2023-12-24T10:23:34+01:00 Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey Berry, DJ Dutton, J Fraser, WD Järvelin, MR Hyppönen, E 2016-09-06 text https://discovery.ucl.ac.uk/id/eprint/1514947/1/Berry_et_al-2016-Journal_of_Clinical_Laboratory_Analysis.pdf https://discovery.ucl.ac.uk/id/eprint/1514947/ eng eng https://discovery.ucl.ac.uk/id/eprint/1514947/1/Berry_et_al-2016-Journal_of_Clinical_Laboratory_Analysis.pdf https://discovery.ucl.ac.uk/id/eprint/1514947/ open Journal of Clinical Laboratory Analysis (2016) (In press). 25(OH)D assay limits of agreement population study reliability replication vitamin D Article 2016 ftucl 2023-11-27T13:07:28Z BACKGROUND: Population-based research on vitamin D has increased dramatically in recent years. Such studies are typically reliant on assay procedures to measure reliable and comparable levels of 25-hydroxyvitamin D [25(OH)D] concentrations. METHODS: Concentrations of 25(OH)D3 and 25(OH)D2 were measured using LC-MS/MS in 5,915 participants (aged 31 years) of Northern Finland Birth Cohort 1966. Blood samples were assayed in batches over a course of 18 months. As anomalies were present in the measurements, 200 samples were reassayed using Diasorin RIA. Agreement between measurements was assessed by Passing-Bablok regression and limits of agreement (LoA). To harmonize LC-MS/MS with Diasorin RIA measurements, formulae were derived from the LoA. RESULTS: Concentrations measured by LC-MS/MS were much higher than those measured by Diasorin RIA, with a mean difference of 12.9 ng/ml. Constant variation was evident between batch measurements after log transformation. Statistical formula was applied separately for each batch of LC-MS/MS measurements, enabling us to remove both the constant and proportional bias that was evident prior to the transformation. CONCLUSION: Despite the introduction of schemes/programs to improve accuracy of assays to measure 25(OH)D, significant differences can still happen. In these instances, methods to harmonize measurements based on a relatively small number of replicates can be successfully applied to establish confidence and to enable between-study comparisons. Article in Journal/Newspaper Northern Finland University College London: UCL Discovery |
institution |
Open Polar |
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University College London: UCL Discovery |
op_collection_id |
ftucl |
language |
English |
topic |
25(OH)D assay limits of agreement population study reliability replication vitamin D |
spellingShingle |
25(OH)D assay limits of agreement population study reliability replication vitamin D Berry, DJ Dutton, J Fraser, WD Järvelin, MR Hyppönen, E Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey |
topic_facet |
25(OH)D assay limits of agreement population study reliability replication vitamin D |
description |
BACKGROUND: Population-based research on vitamin D has increased dramatically in recent years. Such studies are typically reliant on assay procedures to measure reliable and comparable levels of 25-hydroxyvitamin D [25(OH)D] concentrations. METHODS: Concentrations of 25(OH)D3 and 25(OH)D2 were measured using LC-MS/MS in 5,915 participants (aged 31 years) of Northern Finland Birth Cohort 1966. Blood samples were assayed in batches over a course of 18 months. As anomalies were present in the measurements, 200 samples were reassayed using Diasorin RIA. Agreement between measurements was assessed by Passing-Bablok regression and limits of agreement (LoA). To harmonize LC-MS/MS with Diasorin RIA measurements, formulae were derived from the LoA. RESULTS: Concentrations measured by LC-MS/MS were much higher than those measured by Diasorin RIA, with a mean difference of 12.9 ng/ml. Constant variation was evident between batch measurements after log transformation. Statistical formula was applied separately for each batch of LC-MS/MS measurements, enabling us to remove both the constant and proportional bias that was evident prior to the transformation. CONCLUSION: Despite the introduction of schemes/programs to improve accuracy of assays to measure 25(OH)D, significant differences can still happen. In these instances, methods to harmonize measurements based on a relatively small number of replicates can be successfully applied to establish confidence and to enable between-study comparisons. |
format |
Article in Journal/Newspaper |
author |
Berry, DJ Dutton, J Fraser, WD Järvelin, MR Hyppönen, E |
author_facet |
Berry, DJ Dutton, J Fraser, WD Järvelin, MR Hyppönen, E |
author_sort |
Berry, DJ |
title |
Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey |
title_short |
Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey |
title_full |
Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey |
title_fullStr |
Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey |
title_full_unstemmed |
Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey |
title_sort |
harmonization study between lc-ms/ms and diasorin ria for measurement of 25-hydroxyvitamin d concentrations in a large population survey |
publishDate |
2016 |
url |
https://discovery.ucl.ac.uk/id/eprint/1514947/1/Berry_et_al-2016-Journal_of_Clinical_Laboratory_Analysis.pdf https://discovery.ucl.ac.uk/id/eprint/1514947/ |
genre |
Northern Finland |
genre_facet |
Northern Finland |
op_source |
Journal of Clinical Laboratory Analysis (2016) (In press). |
op_relation |
https://discovery.ucl.ac.uk/id/eprint/1514947/1/Berry_et_al-2016-Journal_of_Clinical_Laboratory_Analysis.pdf https://discovery.ucl.ac.uk/id/eprint/1514947/ |
op_rights |
open |
_version_ |
1786197643729305600 |