Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring
Forecasting the impacts of changes in water quality on broadcast spawning aquatic organisms is a key aspect of environmental monitoring. Rapid assays of reproductive potential are central to this monitoring, and there is a need to develop a variety of methods to identify responses. Here, we report a...
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Online Access: | https://discovery.ucl.ac.uk/id/eprint/1473593/8/Falkenberg%20et%20al.,%20Manuscript,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/7/Falkenberg%20et%20al.,%20Highlights,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/4/Falkenberg%20et%20al.,%20Electronic%20Supporting%20Material,%20Sperm%20Accumulated.docx https://discovery.ucl.ac.uk/id/eprint/1473593/ |
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ftucl:oai:eprints.ucl.ac.uk.OAI2:1473593 2023-12-24T10:16:06+01:00 Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring Falkenberg, LJ Havenhand, JN Styan, CS 2016-03 text https://discovery.ucl.ac.uk/id/eprint/1473593/8/Falkenberg%20et%20al.,%20Manuscript,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/7/Falkenberg%20et%20al.,%20Highlights,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/4/Falkenberg%20et%20al.,%20Electronic%20Supporting%20Material,%20Sperm%20Accumulated.docx https://discovery.ucl.ac.uk/id/eprint/1473593/ eng eng https://discovery.ucl.ac.uk/id/eprint/1473593/8/Falkenberg%20et%20al.,%20Manuscript,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/7/Falkenberg%20et%20al.,%20Highlights,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/4/Falkenberg%20et%20al.,%20Electronic%20Supporting%20Material,%20Sperm%20Accumulated.docx https://discovery.ucl.ac.uk/id/eprint/1473593/ open Marine Environmental Research , 114 pp. 51-57. (2016) Bioassay Bioassessment Ecotoxicology Oyster Polychaete Sperm movement Spermatozoa Stickleback Article 2016 ftucl 2023-11-27T13:07:30Z Forecasting the impacts of changes in water quality on broadcast spawning aquatic organisms is a key aspect of environmental monitoring. Rapid assays of reproductive potential are central to this monitoring, and there is a need to develop a variety of methods to identify responses. Here, we report a proof-of-concept study that assesses whether quantification of “Sperm Accumulated Against Surface” (SAAS) of tissue culture well-plates could be a rapid and simple proxy measure of fertilisation success. Our results confirm that motile sperm (but not immotile sperm) actively accumulate at surfaces and that the pattern of accumulation reflects fertilisation success in the model oyster species Crassostrea gigas. Furthermore, we confirm these patterns of SAAS for another marine species, the polychaete Galeolaria caespitosa, as well as for a freshwater species, the fish Gasterosteus aculeatus. For all species considered, SAAS reflected changes in sperm performance caused by experimentally manipulated differences in water quality (here, salinity). These findings indicate that SAAS could be applied easily to a range of species when examining the effects of water quality. Measurement of SAAS could, therefore, form the basis of a rapid and reliable assay for bioassessments of broadcast spawning aquatic organisms. Article in Journal/Newspaper Crassostrea gigas University College London: UCL Discovery |
institution |
Open Polar |
collection |
University College London: UCL Discovery |
op_collection_id |
ftucl |
language |
English |
topic |
Bioassay Bioassessment Ecotoxicology Oyster Polychaete Sperm movement Spermatozoa Stickleback |
spellingShingle |
Bioassay Bioassessment Ecotoxicology Oyster Polychaete Sperm movement Spermatozoa Stickleback Falkenberg, LJ Havenhand, JN Styan, CS Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring |
topic_facet |
Bioassay Bioassessment Ecotoxicology Oyster Polychaete Sperm movement Spermatozoa Stickleback |
description |
Forecasting the impacts of changes in water quality on broadcast spawning aquatic organisms is a key aspect of environmental monitoring. Rapid assays of reproductive potential are central to this monitoring, and there is a need to develop a variety of methods to identify responses. Here, we report a proof-of-concept study that assesses whether quantification of “Sperm Accumulated Against Surface” (SAAS) of tissue culture well-plates could be a rapid and simple proxy measure of fertilisation success. Our results confirm that motile sperm (but not immotile sperm) actively accumulate at surfaces and that the pattern of accumulation reflects fertilisation success in the model oyster species Crassostrea gigas. Furthermore, we confirm these patterns of SAAS for another marine species, the polychaete Galeolaria caespitosa, as well as for a freshwater species, the fish Gasterosteus aculeatus. For all species considered, SAAS reflected changes in sperm performance caused by experimentally manipulated differences in water quality (here, salinity). These findings indicate that SAAS could be applied easily to a range of species when examining the effects of water quality. Measurement of SAAS could, therefore, form the basis of a rapid and reliable assay for bioassessments of broadcast spawning aquatic organisms. |
format |
Article in Journal/Newspaper |
author |
Falkenberg, LJ Havenhand, JN Styan, CS |
author_facet |
Falkenberg, LJ Havenhand, JN Styan, CS |
author_sort |
Falkenberg, LJ |
title |
Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring |
title_short |
Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring |
title_full |
Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring |
title_fullStr |
Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring |
title_full_unstemmed |
Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring |
title_sort |
sperm accumulated against surface: a novel alternative bioassay for environmental monitoring |
publishDate |
2016 |
url |
https://discovery.ucl.ac.uk/id/eprint/1473593/8/Falkenberg%20et%20al.,%20Manuscript,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/7/Falkenberg%20et%20al.,%20Highlights,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/4/Falkenberg%20et%20al.,%20Electronic%20Supporting%20Material,%20Sperm%20Accumulated.docx https://discovery.ucl.ac.uk/id/eprint/1473593/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Environmental Research , 114 pp. 51-57. (2016) |
op_relation |
https://discovery.ucl.ac.uk/id/eprint/1473593/8/Falkenberg%20et%20al.,%20Manuscript,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/7/Falkenberg%20et%20al.,%20Highlights,%20Sperm%20Accumulated%20Against%20Surface.pdf https://discovery.ucl.ac.uk/id/eprint/1473593/4/Falkenberg%20et%20al.,%20Electronic%20Supporting%20Material,%20Sperm%20Accumulated.docx https://discovery.ucl.ac.uk/id/eprint/1473593/ |
op_rights |
open |
_version_ |
1786203409823563776 |