Responses of the Emiliania huxleyi Proteome to Ocean Acidification
Ocean acidification due to rising atmospheric CO2 is expected to affect the physiology of important calcifying marine organisms, but the nature and magnitude of change is yet to be established. In coccolithophores, different species and strains display varying calcification responses to ocean acidif...
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ftucl:oai:eprints.ucl.ac.uk.OAI2:1395631 2023-12-24T10:23:48+01:00 Responses of the Emiliania huxleyi Proteome to Ocean Acidification Jones, BM Iglesias-Rodriguez, MD Skipp, PJ Edwards, RJ Greaves, MJ Young, JR Elderfield, H O'Connor, CD 2013-04-12 application/pdf https://discovery.ucl.ac.uk/id/eprint/1395631/1/journal.pone.0061868.pdf https://discovery.ucl.ac.uk/id/eprint/1395631/ eng eng https://discovery.ucl.ac.uk/id/eprint/1395631/1/journal.pone.0061868.pdf https://discovery.ucl.ac.uk/id/eprint/1395631/ open PLOS ONE , 8 (4) , Article e61868. (2013) Article 2013 ftucl 2023-11-27T13:07:26Z Ocean acidification due to rising atmospheric CO2 is expected to affect the physiology of important calcifying marine organisms, but the nature and magnitude of change is yet to be established. In coccolithophores, different species and strains display varying calcification responses to ocean acidification, but the underlying biochemical properties remain unknown. We employed an approach combining tandem mass-spectrometry with isobaric tagging (iTRAQ) and multiple database searching to identify proteins that were differentially expressed in cells of the marine coccolithophore species Emiliania huxleyi (strain NZEH) between two CO2 conditions: 395 (~current day) and ~1340 p.p.m.v. CO2. Cells exposed to the higher CO2 condition contained more cellular particulate inorganic carbon (CaCO3) and particulate organic nitrogen and carbon than those maintained in present-day conditions. These results are linked with the observation that cells grew slower under elevated CO2, indicating cell cycle disruption. Under high CO2 conditions, coccospheres were larger and cells possessed bigger coccoliths that did not show any signs of malformation compared to those from cells grown under present-day CO2 levels. No differences in calcification rate, particulate organic carbon production or cellular organic carbon: nitrogen ratios were observed. Results were not related to nutrient limitation or acclimation status of cells. At least 46 homologous protein groups from a variety of functional processes were quantified in these experiments, of which four (histones H2A, H3, H4 and a chloroplastic 30S ribosomal protein S7) showed down-regulation in all replicates exposed to high CO2, perhaps reflecting the decrease in growth rate. We present evidence of cellular stress responses but proteins associated with many key metabolic processes remained unaltered. Our results therefore suggest that this E. huxleyi strain possesses some acclimation mechanisms to tolerate future CO2 scenarios, although the observed decline in growth rate may be an ... Article in Journal/Newspaper Ocean acidification University College London: UCL Discovery |
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University College London: UCL Discovery |
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English |
description |
Ocean acidification due to rising atmospheric CO2 is expected to affect the physiology of important calcifying marine organisms, but the nature and magnitude of change is yet to be established. In coccolithophores, different species and strains display varying calcification responses to ocean acidification, but the underlying biochemical properties remain unknown. We employed an approach combining tandem mass-spectrometry with isobaric tagging (iTRAQ) and multiple database searching to identify proteins that were differentially expressed in cells of the marine coccolithophore species Emiliania huxleyi (strain NZEH) between two CO2 conditions: 395 (~current day) and ~1340 p.p.m.v. CO2. Cells exposed to the higher CO2 condition contained more cellular particulate inorganic carbon (CaCO3) and particulate organic nitrogen and carbon than those maintained in present-day conditions. These results are linked with the observation that cells grew slower under elevated CO2, indicating cell cycle disruption. Under high CO2 conditions, coccospheres were larger and cells possessed bigger coccoliths that did not show any signs of malformation compared to those from cells grown under present-day CO2 levels. No differences in calcification rate, particulate organic carbon production or cellular organic carbon: nitrogen ratios were observed. Results were not related to nutrient limitation or acclimation status of cells. At least 46 homologous protein groups from a variety of functional processes were quantified in these experiments, of which four (histones H2A, H3, H4 and a chloroplastic 30S ribosomal protein S7) showed down-regulation in all replicates exposed to high CO2, perhaps reflecting the decrease in growth rate. We present evidence of cellular stress responses but proteins associated with many key metabolic processes remained unaltered. Our results therefore suggest that this E. huxleyi strain possesses some acclimation mechanisms to tolerate future CO2 scenarios, although the observed decline in growth rate may be an ... |
format |
Article in Journal/Newspaper |
author |
Jones, BM Iglesias-Rodriguez, MD Skipp, PJ Edwards, RJ Greaves, MJ Young, JR Elderfield, H O'Connor, CD |
spellingShingle |
Jones, BM Iglesias-Rodriguez, MD Skipp, PJ Edwards, RJ Greaves, MJ Young, JR Elderfield, H O'Connor, CD Responses of the Emiliania huxleyi Proteome to Ocean Acidification |
author_facet |
Jones, BM Iglesias-Rodriguez, MD Skipp, PJ Edwards, RJ Greaves, MJ Young, JR Elderfield, H O'Connor, CD |
author_sort |
Jones, BM |
title |
Responses of the Emiliania huxleyi Proteome to Ocean Acidification |
title_short |
Responses of the Emiliania huxleyi Proteome to Ocean Acidification |
title_full |
Responses of the Emiliania huxleyi Proteome to Ocean Acidification |
title_fullStr |
Responses of the Emiliania huxleyi Proteome to Ocean Acidification |
title_full_unstemmed |
Responses of the Emiliania huxleyi Proteome to Ocean Acidification |
title_sort |
responses of the emiliania huxleyi proteome to ocean acidification |
publishDate |
2013 |
url |
https://discovery.ucl.ac.uk/id/eprint/1395631/1/journal.pone.0061868.pdf https://discovery.ucl.ac.uk/id/eprint/1395631/ |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PLOS ONE , 8 (4) , Article e61868. (2013) |
op_relation |
https://discovery.ucl.ac.uk/id/eprint/1395631/1/journal.pone.0061868.pdf https://discovery.ucl.ac.uk/id/eprint/1395631/ |
op_rights |
open |
_version_ |
1786198046580670464 |